盐酸万古霉素阳离子多囊脂质体的制备及质量考察_《中国修复重建外科杂志》

作者：

杨杜明 ^1,2 , 徐永清 ¹ , 李福兵 ¹ , 刘华 ¹ , 何晓清 ¹

1 成都军区昆明总医院骨科全军创伤骨科研究所（昆明，650032）;;
2昆明医科大学研究生部;

关键词：

盐酸万古霉素阳离子多囊脂质体复乳法正交设计

DOI：

10.7507/1002-1892.20130106

视频：

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目的制备盐酸万古霉素阳离子多囊脂质体（multivesicular liposome，MVL）并对其质量进行考察。方法以复乳法制备盐酸万古霉素阳离子MVL；制备万古霉素储备液，建立万古霉素的体外分析方法，并对方法的专属性、精密度及回吸收率进行评估；采用反相高效液相色谱分析法测定万古霉素药物含量、包封率和体外释放特性；以包封率为主要考察指标，单因素筛选和正交设计优化工艺和处方；光镜和透射电镜下观察脂质体形态；激光散射法测定脂质体的粒径和Zeta电位；动态分析法考察脂质体体外稳定性。结果建立了反相高效液相色谱分析法测定盐酸万古霉素阳离子MVL中万古霉素含量的方法，该方法简便可行，各项方法学符合要求；确定了最优的制备工艺及处方。以最优工艺和处方制备的盐酸万古霉素阳离子MVL外观呈圆形，粒径均一，平均粒径为 3.3 μm，平均包封率为24.9%，平均Zeta电位为24.53 mV；37℃温度下以生理盐水为释放介质，264 h释放药物约为90.5%。将新鲜制备的盐酸万古霉素阳离子MVL放置于4℃冰箱中保存1个月，脂质体稳定性良好。结论以复乳法制备的盐酸万古霉素阳离子MVL外观良好，包封率高，稳定性好，具有良好的缓释效应。

引用本文： 杨杜明,徐永清,李福兵,刘华,何晓清. 盐酸万古霉素阳离子多囊脂质体的制备及质量考察. 中国修复重建外科杂志, 2013, 27(4): 443-448. doi: 10.7507/1002-1892.20130106 复制

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8.	Taylor G, Kellaway IW, Steven J. Drug entrapment and release from multilamellar and reverse phase evaporation liposomes. Int J Pharm, 1990, 58(1): 49-55.
9.	New RRC. Influence of liposome characteristics on their properties and fate//Philippot JR, Schuber F. Liposomes as tools in basic research and industry. Boca Raton: CRC Press, 1995: 3-20.
10.	张淼, 于叶玲, 唐星. 阿糖胞苷多囊脂质体的制备及体外释放度考察. 沈阳药科大学学报, 2009, 26(1): 1-5.
11.	肖超菊, 齐宪荣, 艾尼瓦尔, 等. 顺铂缓释多囊脂质体的制备和体外释放性能研究. 药学学报, 2003, 38(2): 133-137.
12.	马涛, 尚北城, 徐贵丽, 等. 盐酸万古霉素阳离子脂质体的制备及其性质研究. 广东药学院报, 2010, 26(6): 551-555.

1. Sutherland IW. The biofilm matrix: an immobilized but dynamic microbial environment. Trends Microbiol, 2001, 9(5): 222-227.
2. Costerton JW, Stewart PS, Greenberg EP. Bacterial biofilms: a common cause of persistent infections. Science, 1999, 284(5418): 1318-1322.
3. Kim S, Turker MS, Chie Y, et al. Preparation of multivesicular liposomes. Biochim Biophys Acta, 1983, 728(3): 339-348.
4. Mantripragada S. A lipid based depot (DepoFoaml technology) for sustained release drug delivery. Prog Lipid Res, 2002, 41(5): 392-406.
5. Tamilvanan S, Venkateshan N, Ludwig A. The potential of lipid—and polymer-based drug delivery carriers for eradicating biofilm consortia on device-related nosoeomial infections. J Control Release, 2008, 128(1): 20-22.
6. Kim HJ, Gias M, Jones MN. The adsorption of cationic liposomes to Staphylococcus aureus biofilms. Colloids Surf A Physicochem Eng Asp, 1999, 149(1-3): 561-570.
7. Ye Q, Asheman J, Stevenson M, et al. DepoFoam technology a vehicle for controlled delivery of protein and peptide drugs. J Control Release, 2000, 64(1-3): 155-156.
8. Taylor G, Kellaway IW, Steven J. Drug entrapment and release from multilamellar and reverse phase evaporation liposomes. Int J Pharm, 1990, 58(1): 49-55.
9. New RRC. Influence of liposome characteristics on their properties and fate//Philippot JR, Schuber F. Liposomes as tools in basic research and industry. Boca Raton: CRC Press, 1995: 3-20.
10. 张淼, 于叶玲, 唐星. 阿糖胞苷多囊脂质体的制备及体外释放度考察. 沈阳药科大学学报, 2009, 26(1): 1-5.
11. 肖超菊, 齐宪荣, 艾尼瓦尔, 等. 顺铂缓释多囊脂质体的制备和体外释放性能研究. 药学学报, 2003, 38(2): 133-137.
12. 马涛, 尚北城, 徐贵丽, 等. 盐酸万古霉素阳离子脂质体的制备及其性质研究. 广东药学院报, 2010, 26(6): 551-555.

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盐酸万古霉素阳离子多囊脂质体的制备及质量考察

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《中国修复重建外科杂志》

盐酸万古霉素阳离子多囊脂质体的制备及质量考察

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