早期兔骨关节炎软骨CD105+/CD166+细胞及其软骨分化潜能的实验研究_《中国修复重建外科杂志》

作者：

王敏 ¹ , Peter Chen ² , 刘超 ¹ , 郝勇 ¹ , 张瑗 ¹ , 张峡 ¹

1 第三军医大学新桥医院骨科（重庆，400037）;;
2 美国加利福利亚大学圣迭哥分校生物工程系及美国圣迭哥 Scripps骨科研究所;

关键词：

软骨干细胞骨关节炎 CD105 CD166 兔

DOI：

10.7507/1002-1892.20130175

视频：

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摘要 全文 图表 视频 参考文献 施引文献 补充材料

目的探讨早期骨关节炎（osteoarthritis，OA）软骨组织中CD105+/CD166+细胞的变化规律及其软骨分化潜能，为软骨自身修复机制的研究奠定实验基础。方法 8～12月龄成年新西兰大白兔30只，建立右膝OA模型。将实验分为5组，分别获取左膝关节正常软骨细胞（A组），造模后2、4、8周右膝OA软骨细胞（分别为B、C、D组）以及兔BMSCs（E组）。将各组培养细胞标记CD105和CD166，流式细胞仪分析CD105+/CD166+细胞百分比；并采用免疫磁珠分选法将CD105+/CD166+细胞分选出来。激光共聚焦显微镜观察CD105和CD166在各组细胞中的表达分布情况；成软骨诱导培养后分别行阿尔新蓝细胞化学染色和Ⅱ型胶原免疫组织化学染色；成骨诱导培养后检测各组钙含量；成脂诱导培养后行油红O染色。结果 A、B、C、D组CD105+/CD166+细胞百分比显著低于E组（P lt; 0.05）；B、C、D组高于A组（P lt; 0.05），D组显著高于B、C组（P lt; 0.05），但B、C组间差异无统计学意义（P gt; 0.05）。共聚焦显微镜观察示，A、B、C、D组软骨细胞及E组BMSCs中均可见CD105+、CD166+细胞，各组间无明显差异。各组细胞微球成软骨培养1周后，CD105+/CD166+细胞均可见蛋白多糖和Ⅱ型胶原阳性表达，各组间无明显差异。成骨诱导培养2周时，E组钙含量显著高于A、B、C、D组（P lt; 0.05），A、B、C、D组间比较差异无统计学意义（P gt; 0.05）。成脂诱导培养4周，E组可见较多红色脂滴；A、B、C、D组红色脂滴数量较E组少，成片状，大小不一，A、B、C、D组间无差别。结论早期兔OA软骨组织中CD105+/CD166+细胞增多，这些细胞有软骨细胞分化潜能。

引用本文： 王敏,Peter Chen,刘超,郝勇,张瑗,张峡. 早期兔骨关节炎软骨CD105+/CD166+细胞及其软骨分化潜能的实验研究. 中国修复重建外科杂志, 2013, 27(7): 793-799. doi: 10.7507/1002-1892.20130175 复制

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14.	Dowthwaite GP, Bishop JC, Redman SN, et al. The surface of articular cartilage contains a progenitor cell population. J Cell Sci, 2004, 117(Pt 6): 889-897.
15.	Ridley AJ, Schwartz MA, Burridge K, et al. Cell migration: integrating signals from front to back. Science, 2003, 302(5651): 1704-1709.
16.	Kruegel J, Sadowski B, Miosge N. Nidogen-1 and nidogen-2 in healthy human cartilage and in late-stage osteoarthritis cartilage. Arthritis Rheum, 2008, 58(5): 1422-1432.
17.	Saito M, Sasho T, Yamaguchi S, et al. Angiogenic activity of subchondral bone during the progression of osteoarthritis in a rabbit anterior cruciate ligament transection model. Osteoarthritis Cartilage, 2012, 20(12): 1574-1582.
18.	Heida NM, Muller JP, Cheng IF, et al. Effects of obesity and weight loss on the functional properties of early outgrowth endothelial progenitor cells. J Am Coll Cardiol, 2010, 55(4): 357-367.
19.	Sandell LJ, Aigner T. Articular cartilage and changes in arthritis. An introduction: cell biology of osteoarthritis. Artritis Res, 2001, 3(2): 107-113.
20.	Kim HA, Blanco FJ. Cell death and apoptosis in ostearthritic cartilage. Curr Drug Targets, 2007, 8(2): 333-345.
21.	Karlsson C, Stenhamre H, Sandstedt J, et al. Neither Notch1 expression nor cellular size correlate with mesenchymal stem cell properties of adult articular chondrocytes. Cells Tissues Organs, 2008, 187(4): 275-285.
22.	Williams R, Khan IM, Richardson K, et al. Identification and clonal characterisation of a progenitor cell sub-population in normal human articular cartilage. PLoS One, 2010, 5(10): e13246.
23.	Majumdar MK, Thiede MA, Mosca JD, et al. Phenotypic and functional comparison of cultures of marrow derived mesenchymal stem cells (MSCs) and stromal cells. J Cell Physiol, 1998, 176(1): 57-66.

1. Caplan AI. Adult mesenchymal stem cells for tissue engineering versus regenerative medicine. J Cell Physiol, 2007, 213(2): 341-347.
2. Fickert S, Fiedler J, Brenner RE. Identification of subpopulations with characteristics of mesenchymal progenitor cells from human osteoarthritic cartilage using triple staining for cell surface markers. Arthritis Res Ther, 2004, 6(5): R422-432.
3. Alsalameh S, Amin R, Gemba T, et al. Identification of mesenchymal progenitor cells in normal and osteoarthritic human articular cartilage. Arthritis Rheum, 2004, 50(5): 1522-1532.
4. Pretzel D, Linss S, Rochler S, et al. Relative percentage and zonal distribution of mesenchymal progenitor cells in human osteoarthritic and normal cartilage. Arthritis Res Ther, 2011, 13(2): R64.
5. Koelling S, Miosge N. Stem cell therapy for cartilage regeneration in osteoarthritis. Expert Opin Biol Ther, 2009, 9(11): 1399-1405.
6. Koelling S, Miosge N. Sex differences of chondrogenic progenitor cells in late stages of osteoarthritis. Arthritis Rheum, 2010, 62(4): 1077-1087.
7. Koelling S, Kruegel J, Irmer M, et al. Migratory chondrogenic progenitor cells from repair tissue during the later stages of human osteoarthritis. Cell Stem Cell, 2009, 4(4): 324-335.
8. Pickarski M, Hayami T, Zhuo Y, et al. Molecular changes in articular cartilage and subchondral bone in the rat anterior cruciate ligament transection and meniscectomized models of osteoarthritis. BMC Musculoskelet Disord, 2011, 12: 197-212.
9. Johnstone B, Hering TM, Caplan AI, et al. In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells. Exp Cell Res, 1998, 238(1): 265-272.
10. Henrotin Y, Pesesse L, Sanchez C. Subchondral bone and osteoarthritis: biological and cellular aspects. Osteoporos Int, 2012, 23, Suppl 8: S847-851.
11. Bernstein P, Sperling I, Corbeil D, et al. Progenitor cells from cartilageno osteoarthritis-grade-specific differences in stem cell marker expression. Biotechnol Prog, 2013, 29(1): 206-212.
12. Jones BA, Pei M. Synovium-derived stem cells: a tissue-specific stem cell for cartilage engineering and regeneration. Tissue Eng Part B Rev, 2012, 18(4): 301-311.
13. Grogan SP, Miyaki S, Asahara H, et al. Mesenchymal progenitor cell markers in human articular cartilage: normal distribution and changes in osteoarthritis. Arthritis Res Ther, 2009, 11(3): R85.
14. Dowthwaite GP, Bishop JC, Redman SN, et al. The surface of articular cartilage contains a progenitor cell population. J Cell Sci, 2004, 117(Pt 6): 889-897.
15. Ridley AJ, Schwartz MA, Burridge K, et al. Cell migration: integrating signals from front to back. Science, 2003, 302(5651): 1704-1709.
16. Kruegel J, Sadowski B, Miosge N. Nidogen-1 and nidogen-2 in healthy human cartilage and in late-stage osteoarthritis cartilage. Arthritis Rheum, 2008, 58(5): 1422-1432.
17. Saito M, Sasho T, Yamaguchi S, et al. Angiogenic activity of subchondral bone during the progression of osteoarthritis in a rabbit anterior cruciate ligament transection model. Osteoarthritis Cartilage, 2012, 20(12): 1574-1582.
18. Heida NM, Muller JP, Cheng IF, et al. Effects of obesity and weight loss on the functional properties of early outgrowth endothelial progenitor cells. J Am Coll Cardiol, 2010, 55(4): 357-367.
19. Sandell LJ, Aigner T. Articular cartilage and changes in arthritis. An introduction: cell biology of osteoarthritis. Artritis Res, 2001, 3(2): 107-113.
20. Kim HA, Blanco FJ. Cell death and apoptosis in ostearthritic cartilage. Curr Drug Targets, 2007, 8(2): 333-345.
21. Karlsson C, Stenhamre H, Sandstedt J, et al. Neither Notch1 expression nor cellular size correlate with mesenchymal stem cell properties of adult articular chondrocytes. Cells Tissues Organs, 2008, 187(4): 275-285.
22. Williams R, Khan IM, Richardson K, et al. Identification and clonal characterisation of a progenitor cell sub-population in normal human articular cartilage. PLoS One, 2010, 5(10): e13246.
23. Majumdar MK, Thiede MA, Mosca JD, et al. Phenotypic and functional comparison of cultures of marrow derived mesenchymal stem cells (MSCs) and stromal cells. J Cell Physiol, 1998, 176(1): 57-66.

《中国修复重建外科杂志》

早期兔骨关节炎软骨CD105+/CD166+细胞及其软骨分化潜能的实验研究

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《中国修复重建外科杂志》

早期兔骨关节炎软骨CD105+/CD166+细胞及其软骨分化潜能的实验研究

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