脱细胞跟腱复合人成纤维细胞共培养的实验研究_《中国修复重建外科杂志》

作者：

王直兵 , 张峡 , 郭新宇 , 秦川

第三军医大学附属新桥医院骨科（重庆，400037）;

关键词：

组织工程韧带脱细胞跟腱人成纤维细胞细胞相容性兔

DOI：

10.7507/1002-1892.20130177

视频：

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摘要 全文 图表 视频 参考文献 施引文献 补充材料

目的探讨脱细胞跟腱的制备及其与人成纤维细胞复合培养效果，为组织工程韧带的构建提供一种支架材料。方法取10只5月龄雄性新西兰大白兔（体重4～5 kg）双后肢跟腱，经胰蛋白酶、Triton X-100、SDS脱细胞处理后，行大体、组织学及扫描电镜观察。将脱细胞跟腱与人成纤维细胞复合培养，于培养1、3、5、7、9 d行细胞相容性检测，并于4周后取材行组织学、扫描电镜观察以及生物力学检测，并与脱细胞前、后跟腱进行比较。结果跟腱经脱细胞处理后腱膜完整，质柔软、韧性好，体积较处理前跟腱显著增大；跟腱腱束间未见疏松结缔组织及腱细胞，胶原纤维排列疏松，呈三维网状分布，腱束间残留较多孔隙；且细胞相容性良好。复合培养4周后，细胞迁移至纤维束间的孔隙内，三维网状结构消失。生物力学测试示，与脱细胞前跟腱组及细胞-支架复合组比较，脱细胞跟腱组拉伸强度及杨氏弹性模量均显著降低（P lt; 0.05），细胞-支架复合组与脱细胞前跟腱组比较差异无统计学意义（P gt; 0.05）；各组间断裂伸长率比较，差异均无统计学意义（P gt; 0.05）。结论兔脱细胞跟腱与人成纤维细胞生物相容性良好，可作为支架材料用于组织工程韧带构建。

引用本文： 王直兵,张峡,郭新宇,秦川. 脱细胞跟腱复合人成纤维细胞共培养的实验研究. 中国修复重建外科杂志, 2013, 27(7): 805-809. doi: 10.7507/1002-1892.20130177 复制

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25.	Garvin J, Qi J, Maloney M, et al. Novel system for engineering bioartificial tendons and application of mechanical load. Tissue Eng, 2003, 9(5): 967-979.

1. Liu W, Chen B, Deng D, et al. Repair of tendon defect with dermal fibroblast engineered tendon in a porcine model. Tissue Eng, 2006, 12(4): 775-788.
2. Cao D, Liu W, Wei X, et al. In vitro tendon engineering with avian tenocytes and polyglycolic acids: a preliminary report. Tissue Eng, 2006, 12(5): 1369-1377.
3. Whitlock PW, Smith TL, Poehling GG, et al. A naturally derived, cytocompatible, and architecturally optimized scaffold for tendon and ligament regeneration. Biomaterials, 2007, 28(29): 4321-4329.
4. Crapo PM, Gilbert TW, Badylak SF. An overview of tissue and whole organ decellularization processes. Biomaterials, 2011, 32(12): 3233-3243.
5. Brown BN, Valentin JE, Stewart-Akers AM, et al. Macrophage phenotype and remodeling outcomes in response to biologic scaffolds with and without a cellular component. Biomaterials, 2009, 30(8): 1482-1491.
6. 解传飚, 林月秋, 阮默, 等. EDC 交联改性的脱细胞异种(猪)肌腱生物学特性研究. 中国临床解剖学杂志, 2010, 28(4): 425-429.
7. 冷元曦, 林月秋, 阮默, 等. 肌腱移植材料的研究进展. 中国矫形外科杂志, 2009, 17(22): 1704-1706.
8. Yang B, Zhang Y, Zhou L, et al. Development of a porcine bladder acellular matrix with well-preserved extracellular bioactive factors for tissue engineering. Tissue Eng Part C Methods, 2010, 16(5): 1201-1211.
9. 胡成栋, 刘曦, 张伯勋, 等. 脱细胞鸡肌腱的制备与体外生物力学测定的实验研究. 河北医药, 2011, 33(18): 2761-2763.
10. 梁黎明, 柴家科, 杨红明, 等. 脱细胞肌腱制备的实验研究. 中国美容医学, 2006, 15(3): 239-240.
11. Meyer SR, Chiu B, Churchill TA, et al. Comparison of aortic valve allograft decellularization techniques in the rat. J Biomed Mater Res A, 2006, 79(2): 254-262.
12. Cebotari S, Tudorache I, Jaekel T, et al. Detergent decellularization of heart valves for tissue engineering: toxicological effects of residual detergents on human endothelial cells. Artif Organs, 2010, 34(3): 206-210.
13. Feil G, Christ-Adler M, Maurer S, et al. Investigations of urothelial cells seeded on commercially available small intestine submucosa. Eur Urol, 2006, 50(6): 1330-1337.
14. Elder BD, Eleswarapu SV, Athanasiou KA. Extraction techniques for the decellularization of tissue engineered articular cartilage constructs. Biomaterials, 2009, 30(22): 3749-3756．.
15. Ott HC, Matthiesen TS, Goh SK, et al. Perfusion-decellularized matrix: using nature’s platform to engineer a bioartificial heart. Nat Med, 2008, 14(2): 213-221.
16. Uygun BE, Soto-Gutierrez A, Yagi H, et al. Organ reengineering through development of a transplantable recellularized liver graft using decellularized liver matrix. Nat Med, 2010, 16(7): 814-820.
17. Petersen TH, Calle EA, Zhao L, et al. Tissue-engineered lungs for in vivo implantation. Science, 2010, 329(5991): 538-541.
18. Nakayama KH, Batchelder CA, Lee CI, et al. Decellularized rhesus monkey kidney as a three-dimensional scaffold for renal tissue engineering. Tissue Eng Part A, 2010, 16(7): 2207-2216.
19. Ni M, Rui YF, Tan Q, et al. Engineered scaffold-free tendon tissue produced by tendon-derived stem cells. Biomaterials, 2013, 34(8): 2024-2037.
20. Zhang J, Li B, Wang JH. The role of engineered tendon matrix in the stemness of tendon stem cells in vitro and the promotion of tendon-like tissue formation in vivo. Biomaterials, 2011, 32(29): 6972-6981.
21. Hashimoto T, Sun YL, An KN, et al. The effect of tendon surface treatment on cell attachment for potential enhancement of tendon graft healing: an ex vivo model. Med Eng Phys, 2012, 34(10): 1387-1393.
22. Kew SJ, Gwynne JH, Enea D, et al. Regeneration and repair of tendon and ligament tissue using collagen fibre biomaterials. Acta Biomater, 2011, 7(9): 3237-3247.
23. Ikeda J, Zhao C, Chen Q, et al. Compressive properties of cd-HA-gelatin modified intrasynovial tendon allograft in canine model in vivo. Biomechanics, 2011, 44(9): 1793-1796.
24. Wang JH, Guo Q, Li B. Tendon biomechanics and mechanobiology—a minireview of basic concepts and recent advancements. J Hand Ther, 2012, 25(2): 133-141.
25. Garvin J, Qi J, Maloney M, et al. Novel system for engineering bioartificial tendons and application of mechanical load. Tissue Eng, 2003, 9(5): 967-979.

《中国修复重建外科杂志》

脱细胞跟腱复合人成纤维细胞共培养的实验研究

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《中国修复重建外科杂志》

脱细胞跟腱复合人成纤维细胞共培养的实验研究

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