HIV/AIDS实验室检测及其研究进展_《中国循证医学杂志》

作者：

 白浪 , 雷秉钧

四川大学华西医院感染性疾病中心（成都 610041）;

关键词：

获得性免疫缺陷综合征人类免疫缺陷病毒实验室检测

DOI：

10.7507/1672-2531.20080045

视频：

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摘要 全文 图表 视频 参考文献 施引文献 补充材料

获得性免疫缺陷综合征（AIDS）是由人类免疫缺陷病毒（HIV）引起的一种严重危胁人类健康的传染病。实验室检测是诊断HIV/AIDS的主要依据。HIV实验室检测一般包括病毒抗体和抗原检测、病毒核酸检测、免疫细胞检测及病毒耐药检测。20多年来，HIV的实验室诊断方法取得很大进展，特别是近年来免疫学方法和分子生物学方法的应用为临床诊治和流行病学调查提供了有力的帮助。对HIV的检测，灵敏度及特异度高的分析方法将有助于HIV的早期诊断、避免漏检，也有助于对抗艾滋病药物的疗效评价、预测和监测疾病进程，提高检测的灵敏性，缩短检测的窗口期，是HIV诊断方法和诊断试剂持续发展的主要方向。随着对HIV/AIDS研究的不断深入及分子生物学技术的飞速发展，各种检测HIV的新技术层出不穷，HIV的实验室检测正朝着简便、快速、敏感、准确及自动化方向发展。

引用本文： 白浪,雷秉钧. HIV/AIDS实验室检测及其研究进展. 中国循证医学杂志, 2008, 08(3): 206-209. doi: 10.7507/1672-2531.20080045 复制

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2.	Gurtler L, Muhlbacher A, Michl U, et al. Reduction of the diagnostic window with a new combined p24 antigen and human immunodeficiency virus antibody screening assay. J Virol Methods, 1998, 75(1): 27-38.
3.	Weber B, Berger A, Rabenau H, et al. Evaluation of a New Combined Antigen and Antibody Human Immunodeficiency Virus Screening Assay ,VIDAS HIV DUO Ultra. J Clin Microbiol, 2002, 40(4): 1420-1426.
4.	Weber B, Gürtler L, Thorstensson R, et al. Multicenter Evaluation of a New Automated Fourth-generation Human Immunodeficiency Virus Screening Assay with a sensitive Antigen Detection Module and High Specificity. J Clin Microbiol, 2002, 40(6): 1938-1946.
5.	Gurtler LG, Eberle J, Lorbeer B, et al. Sensitivity and specificity of commercial ELISA kits for screening anti-LAV/HTLV III. J Virol Methods, 1987, 15(1): 11-23.
6.	Petricciani JC, Epstein JS. The effects of the AIDS epidemic on the safety of the nation’s blood supply. Public Health Rep, 1988, 103(3): 236-241.
7.	Barbe F, Klein M, Badonnel Y. Early detection of antibodies to human immunodeficiency virus 1 by a third-generation enzyme immunoassay.A comparative study with the results of second-generation immunoassays and western blot. Ann Biol Clin(Paris), 1994, 52(5): 341-345.
8.	Suligoi B, Galli C, Massi M, et al. Precision and Accuracy of a Procedure for Detecting Recent Human Immunodeficiency Virus Infections by Calculating the Antibody Avidity Index by an Automated Immunoassay-Based Method. J Clin. Microbiol, 2002, 40(11): 4015-4020.
9.	Ly TD, Laperche S, Brennan C, et al. Evaluation of the sensitivity and specificity of six HIV combined p24 antigen and antibody assays. J Virol Methods, 2004, 122(2): 185-194.
10.	Bernard M Branson, MD. Rapid Test for HIV Antiboby. AIDS Reviews, 2000, 2: 76-83.
11.	魏经新. 免疫层析试验检测抗HIV1+2的临床应用价值. 中国艾滋病性病, 2003, 9(5): 280-281.
12.	郑晓丽. HIV检测研究进展与发展趋势. 现代检验医学杂志, 2004, 19(6): 64-67.
13.	冯晓英, 徐晓琴. 吸毒人群尿液血液标本HIV-1抗体ELISA检测对比分析. 中国性病艾滋病, 2003, 9(3): 132-133.
14.	赵立庆, 雷素萍, 李连学, 等. ELISA法检测尿液中HIV-1抗体的研究分析. 中国艾滋病性病, 2003, 9(5): 276-277.
15.	Nguyen KA, Busch MP. Evolving strategies for diagnosing human immunodeficiency virus infection. Am J Med, 2000, 109(7): 595-597.
16.	Celum CL, Coombs RW, Jones M, et al. Risk factors for repeatedly reactive HIV-1 EIA and indeterminate western blots. A population-based case-control study. Arch Intern Med, 1994, 154(5): 1129-1137.
17.	Riddler S, Mellors JW. Clinical use of Viral Load Measures. MMWR, April,1998.
18.	肖建春, 郑启唐, 赵之伟. HIV p24抗原检测技术的研究进展. 国外医学病毒学分册, 2003, 10(4): 111-114.
19.	魏民. HIV实验室检测研究进展和发展趋势. 国外医学病毒学分册, 2003, 10(3): 65-69.
20.	Mellors JW, Rinaldo CR, Gupta P, et al. Prognosis in HIV-1 Infection Predicted by the Quantity of virus in plasma. Science, 1996, 272(5): 1167.
21.	Lindback S, Karlsson AC, Mittler J, et al. Viral dynamics in primary HIV-1 infection. AIDS, 2000, 14(15): 2283-2291.
22.	Mellors JW, Munoz A, Giorgi JV, et al. Plasma Viral Load and CD4+ Lymphocytes as Prognostic Markers of HIV-1 Infection. Ann Intern Med, 1997, 126: 946-954.
23.	Abravaya K, Esping C, Hoenle R, et al. Performance of a Multiplex Qualitative PCR LCx Assay for Detection of Human Immunodeficiency Virus Type 1 (HIV-1) Group M Subtypes, Group O, and HIV-2. J Clin Microbiol, 2000, 38(2): 716-723.
24.	Zhao YQ, Min Y, Miller JW, et al. Quantification of Human Immundeficiency virus type 1 Proviral DNA by Using TaqMan Technology. J Chin Microbiol, 2002, 40(2): 675-678.
25.	Leroy V, Montcho C, Manigart O, et al. Maternal plasma viral load, zidovudine and mother-to-child transmission of HIV-1 in Africa:DITRAME ANRS 049a trial. AIDS, 2001, 15(4): 517-522.
26.	Deeks S, Beatty G, Cohen PT, et al. Viral load and CD4+T cells changes in patients failing potent protease inhibitor therapy. Presented at the 5th Conference on Retroviruses and Opportunistic Infections; February 1-5, 1998; Chicago. Abstract 419.
27.	肖瑶, 张可, 裴丽健, 等. 中国HIV/ AIDS 患者CD4+ CD8+T淋巴细胞与外周血各组份间关系的研究. 中国艾滋病性病, 2004, 10(2): 83-88.
28.	Edelstein RE, Nickerson DA, Tobe VO, et al. Oligonucleotide Ligation Assay for Detecting Mutations in the Human Immunodeficiency Virus Type 1 pol Gene That Are Associated with Resistance to Zidovudine, Didanosine, and Lamivudine. J Clin Microbiol, 1998, 36(7): 569-572.

1. Mylonakis E, Paliou M, Lally M, et al. Laboratory testing for infection with the human immunodeficiency virus:established and novel approaches. Am J Med, 2000, 109(7): 568-576.
2. Gurtler L, Muhlbacher A, Michl U, et al. Reduction of the diagnostic window with a new combined p24 antigen and human immunodeficiency virus antibody screening assay. J Virol Methods, 1998, 75(1): 27-38.
3. Weber B, Berger A, Rabenau H, et al. Evaluation of a New Combined Antigen and Antibody Human Immunodeficiency Virus Screening Assay ,VIDAS HIV DUO Ultra. J Clin Microbiol, 2002, 40(4): 1420-1426.
4. Weber B, Gürtler L, Thorstensson R, et al. Multicenter Evaluation of a New Automated Fourth-generation Human Immunodeficiency Virus Screening Assay with a sensitive Antigen Detection Module and High Specificity. J Clin Microbiol, 2002, 40(6): 1938-1946.
5. Gurtler LG, Eberle J, Lorbeer B, et al. Sensitivity and specificity of commercial ELISA kits for screening anti-LAV/HTLV III. J Virol Methods, 1987, 15(1): 11-23.
6. Petricciani JC, Epstein JS. The effects of the AIDS epidemic on the safety of the nation’s blood supply. Public Health Rep, 1988, 103(3): 236-241.
7. Barbe F, Klein M, Badonnel Y. Early detection of antibodies to human immunodeficiency virus 1 by a third-generation enzyme immunoassay.A comparative study with the results of second-generation immunoassays and western blot. Ann Biol Clin(Paris), 1994, 52(5): 341-345.
8. Suligoi B, Galli C, Massi M, et al. Precision and Accuracy of a Procedure for Detecting Recent Human Immunodeficiency Virus Infections by Calculating the Antibody Avidity Index by an Automated Immunoassay-Based Method. J Clin. Microbiol, 2002, 40(11): 4015-4020.
9. Ly TD, Laperche S, Brennan C, et al. Evaluation of the sensitivity and specificity of six HIV combined p24 antigen and antibody assays. J Virol Methods, 2004, 122(2): 185-194.
10. Bernard M Branson, MD. Rapid Test for HIV Antiboby. AIDS Reviews, 2000, 2: 76-83.
11. 魏经新. 免疫层析试验检测抗HIV1+2的临床应用价值. 中国艾滋病性病, 2003, 9(5): 280-281.
12. 郑晓丽. HIV检测研究进展与发展趋势. 现代检验医学杂志, 2004, 19(6): 64-67.
13. 冯晓英, 徐晓琴. 吸毒人群尿液血液标本HIV-1抗体ELISA检测对比分析. 中国性病艾滋病, 2003, 9(3): 132-133.
14. 赵立庆, 雷素萍, 李连学, 等. ELISA法检测尿液中HIV-1抗体的研究分析. 中国艾滋病性病, 2003, 9(5): 276-277.
15. Nguyen KA, Busch MP. Evolving strategies for diagnosing human immunodeficiency virus infection. Am J Med, 2000, 109(7): 595-597.
16. Celum CL, Coombs RW, Jones M, et al. Risk factors for repeatedly reactive HIV-1 EIA and indeterminate western blots. A population-based case-control study. Arch Intern Med, 1994, 154(5): 1129-1137.
17. Riddler S, Mellors JW. Clinical use of Viral Load Measures. MMWR, April,1998.
18. 肖建春, 郑启唐, 赵之伟. HIV p24抗原检测技术的研究进展. 国外医学病毒学分册, 2003, 10(4): 111-114.
19. 魏民. HIV实验室检测研究进展和发展趋势. 国外医学病毒学分册, 2003, 10(3): 65-69.
20. Mellors JW, Rinaldo CR, Gupta P, et al. Prognosis in HIV-1 Infection Predicted by the Quantity of virus in plasma. Science, 1996, 272(5): 1167.
21. Lindback S, Karlsson AC, Mittler J, et al. Viral dynamics in primary HIV-1 infection. AIDS, 2000, 14(15): 2283-2291.
22. Mellors JW, Munoz A, Giorgi JV, et al. Plasma Viral Load and CD4+ Lymphocytes as Prognostic Markers of HIV-1 Infection. Ann Intern Med, 1997, 126: 946-954.
23. Abravaya K, Esping C, Hoenle R, et al. Performance of a Multiplex Qualitative PCR LCx Assay for Detection of Human Immunodeficiency Virus Type 1 (HIV-1) Group M Subtypes, Group O, and HIV-2. J Clin Microbiol, 2000, 38(2): 716-723.
24. Zhao YQ, Min Y, Miller JW, et al. Quantification of Human Immundeficiency virus type 1 Proviral DNA by Using TaqMan Technology. J Chin Microbiol, 2002, 40(2): 675-678.
25. Leroy V, Montcho C, Manigart O, et al. Maternal plasma viral load, zidovudine and mother-to-child transmission of HIV-1 in Africa:DITRAME ANRS 049a trial. AIDS, 2001, 15(4): 517-522.
26. Deeks S, Beatty G, Cohen PT, et al. Viral load and CD4+T cells changes in patients failing potent protease inhibitor therapy. Presented at the 5th Conference on Retroviruses and Opportunistic Infections; February 1-5, 1998; Chicago. Abstract 419.
27. 肖瑶, 张可, 裴丽健, 等. 中国HIV/ AIDS 患者CD4+ CD8+T淋巴细胞与外周血各组份间关系的研究. 中国艾滋病性病, 2004, 10(2): 83-88.
28. Edelstein RE, Nickerson DA, Tobe VO, et al. Oligonucleotide Ligation Assay for Detecting Mutations in the Human Immunodeficiency Virus Type 1 pol Gene That Are Associated with Resistance to Zidovudine, Didanosine, and Lamivudine. J Clin Microbiol, 1998, 36(7): 569-572.

《中国循证医学杂志》

HIV/AIDS实验室检测及其研究进展

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《中国循证医学杂志》

HIV/AIDS实验室检测及其研究进展

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