• 1.中山大學附屬第二醫(yī)院肝膽外科，廣東廣州 510120;2.中山大學附屬江門中心醫(yī)院博士后科研基地，廣東江門 529030;

目的:探討Livin基因在人膽管癌組織及膽管癌細胞系中的表達情況及其與膽管癌發(fā) 生發(fā)展之間的關系。方法:采用免疫組織化學技術（SP法）檢測Livin基因蛋白在45例人膽管 癌標本及及40例癌旁膽管組織、20例正常膽管組織標本中的表達；同時采用RTPCR法及SP 法檢測了Livin基因mRNA和蛋白在人膽管癌細胞系QBC939及非腫瘤細胞系HT1080中表達。 結果:Livin在膽管癌組織中表達陽性率為57.8%，而癌旁膽管組織、非癌膽管組織中未能檢 測到Livin表達。Livin表達與性別、年齡、腫瘤大小及腫瘤分化程度無關。在有淋巴結轉(zhuǎn)組 中，Livin陽性表達率（70.4%）明顯高于無淋巴結轉(zhuǎn)移組（38.9%）。在人膽管癌細胞QBC939 中，Livin mRNA及蛋白均特異性表達，而非腫瘤細胞系HT1080未見Livin表達。結論:Livin 基因在人類膽管癌組織和細胞系中選擇性高表達，其可能與膽管癌發(fā)生、發(fā)展及預后密切相 關。

引用本文： 湯志華,陳積圣,葉林,余杰雄. Livin基因在人膽管癌組織及膽管癌細胞系中的表達和意義. 華西醫(yī)學, 2009, 24(1): 67-69. doi: 復制

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6. Vucic D，Deshayes K，Ackerly H，et al .SMAC negatively regulates the antiapoptotic activity of melanoma inhibitor of apoptosis （MLIAP）[J].J Biol Chem，2002，277 （14）:12275-12279..
7. Kim D K，Alvarado C S，Abramowsky C R，et al.Expression of inhibitorofapoptosis protein （IAP） Livin by neuroblastoma cells:correlation with prognostic factors and outcome[J].Pediatr Dev Pathol，2005，8（6）:621-629.
  1. 1. Kasof GM，Gomes BC.Livin，a novel inhibitor of apoptosis protein family member[J].J Biol Chem，2001，276（5）:3238-3246..
  2. 2. Gazzaniga P，Gradilone A，Giuliani L，et al.Expression and prognostic significance of livin，survivin and other apoptosis related genes in the progression of superficial bladder cancer[J].Ann Oncol，2003，14（1）:85-90..
  3. 3. Yagihashi A，Ohmura T，Asanuma K，et al.Detection of autoantibodies to survivin and livin in sera frompatients with breast cancer[J].Clin Chim Acta，2005，362（12）:125-130..
  4. 4. Burke EC，Jamagin WR，Hoehwald SN，et al.Hilar cholangiocarcinoma:patterns of spread，the importance of hepatic resection for curative operation，and a presurgical clinical staging system[J].Ann Surg，1998，228:385一394..
  5. 5. Isa T，Tomita S，Nakaehi A，et al.Analysis of microsatellite instability，Kras gene mutation and P53 protein over expression intrahepatic cholangiocarcinoma[J]. Hepatogastroenterology，2002，49:604-608..
  6. 6. Vucic D，Deshayes K，Ackerly H，et al .SMAC negatively regulates the antiapoptotic activity of melanoma inhibitor of apoptosis （MLIAP）[J].J Biol Chem，2002，277 （14）:12275-12279..
  7. 7. Kim D K，Alvarado C S，Abramowsky C R，et al.Expression of inhibitorofapoptosis protein （IAP） Livin by neuroblastoma cells:correlation with prognostic factors and outcome[J].Pediatr Dev Pathol，2005，8（6）:621-629.