目的:研究TRAIL對卵巢癌COC1/DDP細(xì)胞生長的影響，以及化療藥物DDP等對TRAIL受體（DR4、DR5）表達(dá)的影響，揭示TRAIL與COC1/DDP細(xì)胞順鉑耐藥性的關(guān)系。方法:用MTT法檢測不同濃度TRAIL蛋白和TRAIL與DDP聯(lián)合用藥對COC1/DDP細(xì)胞生長的影響，用RTPCR方法檢測DDP對TRAIL受體（DR4、DR5）表達(dá)的影響。結(jié)果:①TRAIL蛋白對COC1/DDP細(xì)胞生長有抑制作用，且隨著TRAIL蛋白濃度升高，細(xì)胞抑制率逐漸上升。②DDP（2.5μg/mL）對COC1/DDP細(xì)胞生長抑制作用較弱（抑制率為3.31%），DDP在加入TRAIL蛋白后對細(xì)胞生長抑制率顯著升高（P lt;0.05）。③DDP使COC1/DDP細(xì)胞的DR5表達(dá)水平顯著增強為正常對照組的3.54倍（P lt;0.001）。結(jié)論:TRAIL蛋白對COC1/DDP細(xì)胞生長有抑制作用，DDP與TRAIL聯(lián)合使用COC1/DDP細(xì)胞生長抑制更明顯，TRAIL可逆轉(zhuǎn)COC1/DDP細(xì)胞對DDP的耐藥性，耐藥性的逆轉(zhuǎn)可能與DDP導(dǎo)致TRAIL受體DR5水平增高促進(jìn)了腫瘤細(xì)胞的凋亡有關(guān)。
引用本文： 李春梅,彭芝蘭,尹如鐵. TRAIL逆轉(zhuǎn)人卵巢癌COC1/DDP細(xì)胞對DDP耐藥性的研究. 華西醫(yī)學(xué), 2009, 24(1): 105-107. doi: 復(fù)制
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- 2. Wiley SR，Schooley K，Smolak PJ，et al.Indentification and characterization of a new member of the TNF family that induces apoptosis[J].Immunity，1995，3（6）:673-682..
- 3. Sheridan JP，Marsters SA，Pitti RM，et al.Control of TRAILinduced apoptosis by a family of signaling and decoy receptors[J].Science，1997，277（5327）:818-821..
- 4. Sprick MR，Weigand MA，Rieser E，et al.FADD/MORT1 and caspase8 are recruited to TRAIL receptors 1 and 2 and are essential for apoptosis mediated by TRAIL receptor 2[J].Immunity，2000，12（6）:599-609..
- 5. Thomas LR，Henson A，Reed JC，et al.Direct binding of Fasassociated death domain （FADD） to the tumor necrosis factorrelated apoptosisinducing ligand receptor DR5 is regulated by the death effector domain of FADD[J].J Biol Chem，2004，279（31）:32780-32785..
- 6. 李春梅，彭芝蘭，尹如鐵.TRAIL蛋白對卵巢癌COC1和COC1/DDP細(xì)胞生長及促凋亡作用研究[J].四川腫瘤防治，2006，19（3）:161-166..
- 7. Cuello M，Ettenberg SA，Nau MM，et al.Synergistic induction of apoptosis by the combination of TRAIL and chemotherapy in chemoresistant ovarian cancer cells[J].Gynecol Oncol，2001，81（3）:380-390..
- 8. Arts HJ，de Jong S，Hollema H，et al.Chemotherapy induces death receptor 5 in epithelial ovarian carcinoma[J].Gynecol Oncol，2004，92（3）:794-800..
- 9. Tomek S，Horak P，Pribill I，et al.Resistance to TRAILinduced apoptosis in ovarian cancer cell lines is overcome by cotreatment with cytotoxic drugs[J].Gynecol Oncol，2004，94（1）:107-114..