• 1成都醫(yī)學(xué)院病理教研室，四川成都 610083;2綿陽(yáng)市中心醫(yī)院生殖中心，四川綿陽(yáng) 621000;3四川大學(xué)華西醫(yī)學(xué)中心生理學(xué);

目的：探討雌激素影響人精子頂體反應(yīng)的可能機(jī)制。方法：應(yīng)用異硫氰酸熒光素標(biāo)記豌豆凝集素?zé)晒馊旧ǎ‵ITC-PSA）分析精子頂體反應(yīng)（AR）、以分光光度比色法測(cè)定頂體酶（ACE）活性。結(jié)果：17β-雌二醇（17β-E2）可促進(jìn)精子發(fā)生AR，并增強(qiáng)精子頂體酶的活性；去除培養(yǎng)液中的Ca2+后，17β-E2不能誘導(dǎo)精子發(fā)生AR；PKC抑制劑能明顯降低17β-E2所誘導(dǎo)的AR；E2-BSA亦能夠促進(jìn)精子發(fā)生AR，其作用與17β-E2無(wú)顯著差異。結(jié)論：雌激素對(duì)人精子頂體反應(yīng)有一定的促進(jìn)作用，增強(qiáng)精子頂體酶活性可能是其作用途徑之一，此過程涉及了胞外Ca2+、PKC及精子膜上的ER或雌激素結(jié)合位點(diǎn)的參與。

引用本文： 何彥芳,鄭潔,岳利民,何亞平,張金虎,王 嶸. 17β-雌二醇影響人精子頂體反應(yīng)機(jī)制的初步研究. 華西醫(yī)學(xué), 2008, 23(1): 34-35. doi: 復(fù)制

1. Serge Carreau,Sonia Bourguiba,Sophie Lambard,et al.Reproductive system:aromatase and estrogens[J].Molecular and Cellular Endocrinology,2002,193(1-2):137-143.
2. World Health Organization.WHO laboratory manual for the exanlination of human sperm and sperm-cervical mucus interaction[M].4th ed.New York:Cambridge University Press,2001.
3. Revelli A,Costamagna C,Mofa F,et a1.Signaling pathway of nitric oxide-induced acrosome reaction in human spermatozoa[J].Biol Reprod,2001,64(6):1708-1712.
4. 肖春花，王忠山，左文靜，等．精子頂體酶活性檢測(cè)新方法與臨床應(yīng)用[J]．男性學(xué)雜志，1994，8(4)：98-200.
5. Aquila S,Sisci D,Gentile M,et al.Towards a physiological role for cytochrome P450 aromatase in ejaculated human sperm[J].Hum Reprod,2003,18(8):1650-1659.
6. Cui YH,Zhao RL,Wang Q,et a1.Determination of sperm acrosin activity for evaluation of male fertility[J].Asian J Andm1,2000,2(3):229-232.
7. Breitbart H.Intracellular calcium regulation in sperm capacitation and acrosomal reaction[J].Mol Cell Endocrinol,2002,187(1-2):139-144.
8. Patrat C,Serresc C,Jounnet P.The aerosome reaction in human spermatozoa.Biol Cell,2000,92(3-4):255-266.
9. Luconi M,Francavilla F,Porazzi I,et a1.Human spermatozoa as a model for studying membrane receptors mediating rapid nongenomic effects of progesterone and estrogens[J].Steroids,2004,69(8-9):553-559.
  1. 1. Serge Carreau,Sonia Bourguiba,Sophie Lambard,et al.Reproductive system:aromatase and estrogens[J].Molecular and Cellular Endocrinology,2002,193(1-2):137-143.
  2. 2. World Health Organization.WHO laboratory manual for the exanlination of human sperm and sperm-cervical mucus interaction[M].4th ed.New York:Cambridge University Press,2001.
  3. 3. Revelli A,Costamagna C,Mofa F,et a1.Signaling pathway of nitric oxide-induced acrosome reaction in human spermatozoa[J].Biol Reprod,2001,64(6):1708-1712.
  4. 4. 肖春花，王忠山，左文靜，等．精子頂體酶活性檢測(cè)新方法與臨床應(yīng)用[J]．男性學(xué)雜志，1994，8(4)：98-200.
  5. 5. Aquila S,Sisci D,Gentile M,et al.Towards a physiological role for cytochrome P450 aromatase in ejaculated human sperm[J].Hum Reprod,2003,18(8):1650-1659.
  6. 6. Cui YH,Zhao RL,Wang Q,et a1.Determination of sperm acrosin activity for evaluation of male fertility[J].Asian J Andm1,2000,2(3):229-232.
  7. 7. Breitbart H.Intracellular calcium regulation in sperm capacitation and acrosomal reaction[J].Mol Cell Endocrinol,2002,187(1-2):139-144.
  8. 8. Patrat C,Serresc C,Jounnet P.The aerosome reaction in human spermatozoa.Biol Cell,2000,92(3-4):255-266.
  9. 9. Luconi M,Francavilla F,Porazzi I,et a1.Human spermatozoa as a model for studying membrane receptors mediating rapid nongenomic effects of progesterone and estrogens[J].Steroids,2004,69(8-9):553-559.