• 四川大學(xué)華西醫(yī)院 1呼吸內(nèi)科;2實驗醫(yī)學(xué)科，四川成都 610041;

研究白介素6(IL-6)在過氧化氫誘導(dǎo)的肺泡上皮細(xì)胞凋亡中的作用及其機制。方法：以人Ⅱ型肺泡上皮細(xì)胞A549細(xì)胞株為實驗對象，MTT法檢測IL-6對A549細(xì)胞增殖活性的影響，TUNEL檢測法檢測IL-6對A549細(xì)胞凋亡率的影響，Western blot觀察caspase-3和caspase-9蛋白量的變化。結(jié)果：IL-6能夠增加A549細(xì)胞的增殖活性，減少其凋亡。與模型組相比，IL-6干預(yù)組的caspase-3和caspase-9的蛋白表達(dá)量明顯降低,有統(tǒng)計學(xué)意義。結(jié)論：IL-6能夠抑制過氧化氫誘導(dǎo)的肺泡上皮細(xì)胞凋亡，并且與caspase-3和caspase-9蛋白的表達(dá)量下降有關(guān)。提示IL-6對肺泡上皮細(xì)胞的保護作用可能是通過抑制細(xì)胞凋亡相關(guān)蛋白來實現(xiàn)的。

引用本文： 楊冬梅,丁宇,應(yīng)斌武,蔣飛,美朗曲措,范紅. 白介素6對過氧化氫誘導(dǎo)的肺泡上皮細(xì)胞凋亡的影響. 華西醫(yī)學(xué), 2008, 23(1): 98-100. doi: 復(fù)制

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