• 四川大學華西醫(yī)院眼科中心,四川成都 610041;

目的：研究轉化生長因子β1（TGF-β1）促進Tenon囊成纖維細胞的增殖作用及核心蛋白多糖（decorin）對TGF-β1促Tenon囊成纖維細胞增殖的影響。方法：（1）取青光眼患者的Tenon囊組織，采用組織塊貼壁培養(yǎng)法進行原代培養(yǎng)細胞培養(yǎng)，細胞經(jīng)過冷凍與復蘇后，觀察細胞形態(tài)結構與生物學特性。（2）用不同濃度(0.01、0.1、1、5、10ng/mL)TGF-β1作用于Tenon囊成纖維細胞48 h。（3）終濃度為5 ng/mL的TGF-β1 和不同濃度(0.01、0.1、1、2、5μg/mL)的decorin共同作用于Tenon囊成纖維細胞48h。MTT比色法檢測Tenon囊成纖維細胞增殖。結果：（1）體外成功培養(yǎng)Tenon囊成纖維細胞，細胞生長活躍，形狀穩(wěn)定，細胞經(jīng)過冷凍與復蘇后，仍能保持其原來的細胞形態(tài)結構與穩(wěn)定的生物學特性。（2）1～10ng/mL TGF-β1能促進Tenon囊成纖維細胞的增殖，并與濃度成正比。（3）2～5μg/mL decorin 能抑制TGF-β1促Tenon囊成纖維細胞增殖作用，其抑制作用與濃度成正比。結論： TGF-β1可刺激Tenon囊成纖維細胞增殖，Decorin能抑制TGF-β1促進細胞增殖的作用，其機理可能通過與TGF-β1結合抑制青光眼濾過手術后瘢痕形成。

引用本文： 盤如剛,陳曉明,劉東敬,李茅. 核心蛋白多糖對TGF-β1促Tenon囊成纖維細胞增殖的影響. 華西醫(yī)學, 2008, 23(1): 101-102. doi: 復制

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  1. 1. Brogliatti B,Rigault R,Palanza L,et al.Intraocular pressure and progression of visual field damage[J].Acta Ophthalmol Scand Suppl,2002,236:26-27．.
  2. 2. Francis BA,Du LT,Najafi K,et al.Histopathologic features of conjunctival filtering blebs [J].Arch Ophthalmol,2005,123:166-170．.
  3. 3. Nomu Y.Structural change in decorin with skin aging[Ｊ].Connect Tissue Res,2006,47(5):249-255．.
  4. 4. Nash MA,Loercher AE,Freedman RS.In vitrogrowth inhibition of ovarian cancer cells by decorin:synergism of action between decorin and carboplatin.Cancer Res，1999,59:6192-6196..
  5. 5. Choi WH,Ji KA,Jeon SB,et al.Anti-inflammatory roles of retinoic acid in rat brain astrocytes:Suppression of interferon-γ-induced JAK/STATphosphorylation[Ｊ].Biochem Biophys Res Commun,2005,329(1):125-131..
  6. 6. Corderio MF.Beyond mitomycin:TGF-β and wound healing[J].Prog Reyin Eye Res,2002,21:75-89．.
  7. 7. Tripathi RC,Li J,Chalam KV,et al.Expression of growth factor mRNAs by human Tenon’s capsule fibroblasts[Ｊ].Exp Eye Res,1996,63:339-346.
  8. 8. Moustakas A,Souchelnytskyi S,Heldin CH.Smad regulation in TGF-beta singal transduction[Ｊ].J Cell Sci，2001，114(Pt24):4359-4369..
  9. 9. Kolb M,Margetts PJ,Sime PJ,et al.Proteoglycans decorin and biglycan differentially modulate TGF-β-mediated fibrotic responses in the lung[Ｊ].Am J Physiol Lung Cell Mol Physiol，2001，280:L1327-Ｌ1334.