• 1.青島大學醫(yī)學院（山東青島 266071）;;
  • 2.青島大學醫(yī)學院附屬煙臺毓璜頂醫(yī)院肝膽胰外科（山東煙臺 264000）;

目的研究hTERT mRNA 及 GGT mRNA-H 在小肝癌患者外周血中的表達,并探討其對肝癌早期診斷的價值。方法采用RT-PCR方法檢測30例小肝癌患者、18例肝炎肝硬變患者以及12例正常受試者外周血hTERT mRNA及GGT mRNAH的表達。結果小肝癌組患者hTERT mRNA及GGT mRNAH表達陽性率分別為80.0%（24/30）和46.7%（14/30），均高于AFP的檢測陽性率(26.7%,8/30)，P lt;0.05。正常對照組均未檢測到hTERT mRNA和GGT mRNAH的表達。肝炎肝硬變組患者hTERT mRNA的表達陽性率為33.3%（6/18），未檢測到GGT mRNAH的表達。 聯(lián)合檢測小肝癌患者hTERT mRNA及GGT mRNA-H表達的陽性率明顯高于AFP的陽性率（86.7%比26.7%），P lt;0.05。結論hTERT mRNA及GGT mRNA-H在小肝癌患者中特異性表達，聯(lián)合檢測外周血hTERT mRNA及GGT mRNAH 可提高小肝癌的早期診斷率。

引用本文： 隋璐璐,劉小方,許政. 聯(lián)合檢測外周血hTERT mRNA及GGT mRNA-H表達對小肝癌早期診斷的價值. 中國普外基礎與臨床雜志, 2011, 18(1): 59-62. doi: 復制

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2. Zhang Y, Li Q, Liu N, et al. Detection of MAGE1, MAGE3 and AFP mRNA as multimarker by realtime quantitative PCR assay: a possible predictor of hematogenous micrometastasis of hepatocellular carcinoma [J]. Hepatogastroenterology, 2008, 55(88): 22002206.
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6. Miura N, Osaki Y, Nagashima M, et al. A novel biomarker TERTmRNA is applicable for early detection of hepatoma [J]. BMC Gastroenterol, 2010, 10: 4657.
7. Wang NY, Zhang D, Zhao W, et al. Clinical application of an enzymelinked immunosorbent assay detecting hepatomaspecific γglutamyltransferase [J]. Hepatol Res, 2009, 39(10): 979987.
8. 中華醫(yī)學會傳染病與寄生蟲病學分會、肝病學分會. 病毒性肝炎防治方案 [J]. 中華肝臟病雜志, 2000, 8(6): 324329.
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11. Terrin L, Rampazzo E, Pucciarelli S. et al. Relationship between tumor and plasma levels of hTERT mRNA in patients with colorectal cancer: implications for monitoring of neoplastic disease [J]. Clin Cancer Res, 2008, 14(22): 74447451.
12. Gigek CO, Leal MF, Silva PN, et al. hTERT methylation and expression in gastric cancer [J]. Biomarkers, 2009, 14(8): 630636.
13. Udomchaiprasertkul W, Narong S, Kongsema M, et al. Detection of hTERT mRNA in gastrointestinal tract cancer specimens [J]. Southeast Asian J Trop Med Public Health, 2008, 39(2): 324327.
14. Pasrija T, Srinivasan R, Behera D, et al. Telomerase activity in sputum and telomerase and its components in biopsies of advanced lung cancer [J]. Eur J Cancer, 2007, 43(9): 14761482.
15. Capezzone M, Marchisotta S, Cantara S, et al. Telomeres and thyroid cancer [J]. Curr Genomics, 2009, 10(8): 526533.
16. Perona R, MachadoPinilla R, Manguan C, et al. Telomerase deficiency and cancer susceptibility syndromes [J]. Clin Transl Oncol, 2009, 11(11): 711714.
17. Stewart SA, Bertuch AA. The role of telomeres and telomerase in cancer research [J]. Cancer Res, 2010, 70(19): 73657371.
18. Shay JW, Wright WE. Telomeres and telomerase in normal and cancer stem cells [J]. FEBS Lett, 2010, 584(17): 38193825.
19. Hashimoto Y, Murakami Y, Uemura K, et al. Detection of human telomerase reverse transcriptase (hTERT) expression in tissue and pancreatic juice from pancreatic cancer [J]. Surgery, 2008, 143(1): 113125.
20. Bravaccini S, Casadio V, Amadori D, et al. The current role of telomerase in the diagnosis of bladder cancer [J]. Indian J Urol, 2009, 25(1): 4046.
21. Varela E, Blasco MA. 2009 nobel prize in physiology or medicine: telomeres and telomerase [J]. Oncogene, 2010, 29(11): 15611565.
22. Theimer CA, Feigon J. Structure and function of telomerase RNA [J]. Curr Opin Struct Biol, 2006, 16(3): 307318.
23. Han GQ, Qin CY, Shu RH. The analysis of γglutamyl transpeptidase gene in different type liver tissues [J]. World J Gastroenterol, 2003, 9(2): 276280.
24. Tsutsumi M, Sakamuro D, Takada A, et al. Detection of a unique γglutamyl transpeptidase messenger RNA species closely related to the development of hepatocellular carcinoma in humans: a new candidate for early diagnosis of hepatocellular carcinoma [J]. Hepatology, 1996, 23(5): 10931097.
25. Daubeuf S, Accaoui MJ, Pettersen I, et al. Differential regulation of γglutamyltransferase mRNAs in four human tumour cell lines [J]. Biochim Biophys Acta, 2001, 1568(1): 6773.
26. 徐守偉, 劉曉華, 韓國慶, 等. 原發(fā)性肝癌患者外周血、肝組織中GGT mRNAH 表達及意義 [J]. 山東醫(yī)藥, 2009, 49(7): 6061.
  1. 1. Llovet JM, Bruix J. Novel advancements in the management of hepatocellular carcinoma in 2008 [J]. J Hepatol, 2008, 48 Suppl 1: S20S37.
  2. 2. Zhang Y, Li Q, Liu N, et al. Detection of MAGE1, MAGE3 and AFP mRNA as multimarker by realtime quantitative PCR assay: a possible predictor of hematogenous micrometastasis of hepatocellular carcinoma [J]. Hepatogastroenterology, 2008, 55(88): 22002206.
  3. 3. 錢林學, 劉玉江, 丁惠國. 小肝癌的影像學診斷進展 [J]. 世界華人消化雜志, 2010, 18(5): 479486.
  4. 4. 陳孝平, 張萬廣. 原發(fā)性肝癌面臨的難點與對策 [J]. 中國普外基礎與臨床雜志， 2008, 15(4): 231233.
  5. 5. 沈嘯洪, 李慧鍇, 張倜. 肝癌外科規(guī)范化治療若干問題探討 [J]. 中國普外基礎與臨床雜志, 2009, 16(4): 261264.
  6. 6. Miura N, Osaki Y, Nagashima M, et al. A novel biomarker TERTmRNA is applicable for early detection of hepatoma [J]. BMC Gastroenterol, 2010, 10: 4657.
  7. 7. Wang NY, Zhang D, Zhao W, et al. Clinical application of an enzymelinked immunosorbent assay detecting hepatomaspecific γglutamyltransferase [J]. Hepatol Res, 2009, 39(10): 979987.
  8. 8. 中華醫(yī)學會傳染病與寄生蟲病學分會、肝病學分會. 病毒性肝炎防治方案 [J]. 中華肝臟病雜志, 2000, 8(6): 324329.
  9. 9. ElSerag HB, Rudolph KL. Hepatocellular carcinoma: epidemiology and molecular carcinogenesis [J]. Gastroenterology, 2007, 132(7): 25572576.
  10. 10. Liu H, Li P, Zhai Y, et al. Diagnostic value of glypican3 in serum and liver for primary hepatocellular carcinoma [J]. World J Gastroenterol, 2010, 16(35): 44104415.
  11. 11. Terrin L, Rampazzo E, Pucciarelli S. et al. Relationship between tumor and plasma levels of hTERT mRNA in patients with colorectal cancer: implications for monitoring of neoplastic disease [J]. Clin Cancer Res, 2008, 14(22): 74447451.
  12. 12. Gigek CO, Leal MF, Silva PN, et al. hTERT methylation and expression in gastric cancer [J]. Biomarkers, 2009, 14(8): 630636.
  13. 13. Udomchaiprasertkul W, Narong S, Kongsema M, et al. Detection of hTERT mRNA in gastrointestinal tract cancer specimens [J]. Southeast Asian J Trop Med Public Health, 2008, 39(2): 324327.
  14. 14. Pasrija T, Srinivasan R, Behera D, et al. Telomerase activity in sputum and telomerase and its components in biopsies of advanced lung cancer [J]. Eur J Cancer, 2007, 43(9): 14761482.
  15. 15. Capezzone M, Marchisotta S, Cantara S, et al. Telomeres and thyroid cancer [J]. Curr Genomics, 2009, 10(8): 526533.
  16. 16. Perona R, MachadoPinilla R, Manguan C, et al. Telomerase deficiency and cancer susceptibility syndromes [J]. Clin Transl Oncol, 2009, 11(11): 711714.
  17. 17. Stewart SA, Bertuch AA. The role of telomeres and telomerase in cancer research [J]. Cancer Res, 2010, 70(19): 73657371.
  18. 18. Shay JW, Wright WE. Telomeres and telomerase in normal and cancer stem cells [J]. FEBS Lett, 2010, 584(17): 38193825.
  19. 19. Hashimoto Y, Murakami Y, Uemura K, et al. Detection of human telomerase reverse transcriptase (hTERT) expression in tissue and pancreatic juice from pancreatic cancer [J]. Surgery, 2008, 143(1): 113125.
  20. 20. Bravaccini S, Casadio V, Amadori D, et al. The current role of telomerase in the diagnosis of bladder cancer [J]. Indian J Urol, 2009, 25(1): 4046.
  21. 21. Varela E, Blasco MA. 2009 nobel prize in physiology or medicine: telomeres and telomerase [J]. Oncogene, 2010, 29(11): 15611565.
  22. 22. Theimer CA, Feigon J. Structure and function of telomerase RNA [J]. Curr Opin Struct Biol, 2006, 16(3): 307318.
  23. 23. Han GQ, Qin CY, Shu RH. The analysis of γglutamyl transpeptidase gene in different type liver tissues [J]. World J Gastroenterol, 2003, 9(2): 276280.
  24. 24. Tsutsumi M, Sakamuro D, Takada A, et al. Detection of a unique γglutamyl transpeptidase messenger RNA species closely related to the development of hepatocellular carcinoma in humans: a new candidate for early diagnosis of hepatocellular carcinoma [J]. Hepatology, 1996, 23(5): 10931097.
  25. 25. Daubeuf S, Accaoui MJ, Pettersen I, et al. Differential regulation of γglutamyltransferase mRNAs in four human tumour cell lines [J]. Biochim Biophys Acta, 2001, 1568(1): 6773.
  26. 26. 徐守偉, 劉曉華, 韓國慶, 等. 原發(fā)性肝癌患者外周血、肝組織中GGT mRNAH 表達及意義 [J]. 山東醫(yī)藥, 2009, 49(7): 6061.

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