• 中國(guó)醫(yī)科大學(xué)附屬盛京醫(yī)院胃腸外科（沈陽(yáng) 110004）;

目的  探討胃癌組織中第10號(hào)染色體同源缺失性磷酸酶-張力蛋白（PTEN）、Fas/FasL和基質(zhì)金屬蛋白酶-2（MMP-2）的表達(dá)及其與胃癌生物學(xué)行為的關(guān)系。方法  選擇臨床及病理資料齊全的胃癌蠟塊標(biāo)本75例，另取正常胃黏膜組織15例作對(duì)照，采用SP免疫組化方法檢測(cè)PTEN、Fas/FasL和MMP-2在其中的表達(dá)。采用χ2檢驗(yàn)和相關(guān)分析作統(tǒng)計(jì)學(xué)分析。結(jié)果  PTEN、Fas/FasL及MMP-2的表達(dá)與胃癌的浸潤(rùn)深度、淋巴結(jié)轉(zhuǎn)移、TNM分期及腫瘤的組織分化程度均有關(guān)（P lt;0.05）。PTEN和Fas/FasL的表達(dá)呈正相關(guān)（r=0.401，P lt;0.001），MMP-2與Fas/FasL的表達(dá)呈負(fù)相關(guān)（r=－0.720，P lt;0.001），MMP-2與PTEN的表達(dá)呈負(fù)相關(guān)（r=－0.336，P lt;0.001）。結(jié)論  胃癌組織中PTEN、Fas/FasL及MMP-2的表達(dá)陽(yáng)性率可以成為胃癌診斷和預(yù)后的判斷指標(biāo)。

引用本文： 王鵬,郭琳,王強(qiáng). PTEN、Fas/FasL和MMP-2在胃癌組織的表達(dá)及其臨床意義. 中國(guó)普外基礎(chǔ)與臨床雜志, 2009, 16(12): 991-995. doi: 復(fù)制

1. Li J, Yen C, Liaw D, et al. PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer [J]. Science, 1997； 275(5308): 1943-1947.
2. Li DM, Sun H. TEP1, encoded by a candidate tumor suppressor locus, is a novel protein tyrosine phosphatase regulated by transforming growth factor beta [J]. Cancer Res, 1997； 57 (11): 2124-2129.
3. Steck PA, Pershouse MA, Jasser SA, et al. Identification of a candidate tumor suppressor gene MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers [J]. Nat Genet, 1997； 15(4): 356-362.
4. 熊敏, 吳一龍主編. 現(xiàn)代肺癌病理與臨床 [M]. 北京： 北京科學(xué)出版社 ，2003: 97-98.
5. Zhao MD, Hu XM, Sun DJ, et al. Expression of some tumor associated factors in human carcinogenesis and development of gastric carcinoma [J]. World J Gastroenterol, 2005； 11 (21): 3217-3221.
6. Forsyth PA, Wong H, Laing TD, et al. Gelatinase-A (MMP-2), gelatinase-B (MMP-9) and membrane type matrix metalloproteinase-1 (MT1-MMP) are involved in different aspects of the pathophysiology of malignant gliomas [J]. Br J Cancer, 1999； 79 (11-12): 1828-1835.
7. Yoo CH, Noh SH, Kim YI, et al. Comparison of prognostic significance of nodal staging between old (4th edition) and new (5th edition) UICC TNM classification for gastric carcinoma. International Union Against Cancer [J]. World J Surg, 1999; 23(5): 492-497.
8. Jawhari A, Jordan S, Poole S, et al. Abnormal immunoreactivity of the E-cadherin-catenin complex in gastric carcinoma: relationship with patient survival [J]. Gastroenterology， 1997; 112(1): 46-54.
9. 陳昊, 李玉民, 何明彥, 等. 胃不典型增生和癌組織中Ki67和PTEN蛋白的表達(dá)及意義 [J]. 中國(guó)普外基礎(chǔ)與臨床雜志， 2007； 14（3）： 304-307.
10. Tamura M, Gu J, Takino T, et al. Tumor suppressor PTEN inhibition of cell invasion, migration and growth: differential involvement of focal adhesion kinase and pl30Cas [J]. Cancer Res, 1999； 59(2): 442-449.
11. Yang L, Kuang LG, Zheng HC, et al. PTEN encoding product: a marker for tumorigenesis and progression of gastric carcinoma [J]. World J Gastroenterol, 2003； 9(1): 35-39.
12. Fei G, Ebert MP, Mawrin C, et al. Reduced PTEN expression in gastric cancer and in the gastric mucosa of gastric cancer relatives [J]. Eur J Gastroenterol Hepatol, 2002； 14(3): 297-303.
13. 李海民, 竇科峰, 李韌, 等. 肝癌組織中Fas和FasL mRNA表達(dá)水平的變化及其意義 [J]. 中國(guó)普外基礎(chǔ)與臨床雜志， 2005； 12（3）： 284-286.
14. 何貴金, 于發(fā)強(qiáng), 高沁怡, 等. 103鈀放射性支架對(duì)Fas基因表達(dá)影響及與膽管癌細(xì)胞凋亡關(guān)系研究 [J]. 中國(guó)普外基礎(chǔ)與臨床雜志， 2007； 14（3）： 284-286.
15. Kusakabe T, Nashimoto A, Honma K, et al. Fatty acid synthase is highly expressed in carcinoma, adenoma and in regenerative epithelium and intestinal metaplasia of the stomach [J]. Histopathology, 2002； 40(1): 71-79.
16. Morimoto Y, Hizuta A, Ding EX, et al. Funtional expression of Fas and Fas ligand on human intestinal intraepithelial lymphocytes [J]. Clin Exp immuno1, 1999； 116(1): 84-89.
17. 項(xiàng)良光, 林新龍, 偉青, 等. 耐阿霉素肝癌細(xì)胞HepG2表達(dá)Fas的變化 [J]. 中國(guó)普外基礎(chǔ)與臨床雜志， 2000； 7（4）： 229-230.
18. Elnemr A, Ohta T, Yachie A, et al. Human pancreatic cancer cells disable function of Fas receptors at several levels in Fas signal transduction pathway [J]. Int J Oncol, 2001； 18(2): 311-316.
19. Nabeshima K, Inoue T, Shimao Y, et al． Front-cells- specific expression of membrane-type 1 matrix metalloproteinase and gelatinase A during cohort migration of colon carcinoma cells induced by hepatocyte growth factor／scatter factor [J]． Cancer Res, 2000； 60(13): 3364-3369.
20. Grigioni WF, D’Errico A, Fiorentino M, et al. Gelatinase A (MMP-2) and its mRNA detected in both neoplastic and stromal cells of tumors with different invasive and metastatic properties [J]． Diagn Mol Pathol, 1994； 3(3): 163-169.
21. Nomura H, Fujimoto N, Seiki M, et al. Enhanced production of matrix metalloproteinases and activation of matrix metalloproteinase 2 (gelatinase A) in human gastric carcinomas [J]. Int J Cancer, 1996； 69(1): 9-16.
22. Ko BK, Cho HR, Choi DW, et al. Reduced expression of tissue inhibitor of metalloproteinase in nodal metastasis of stomach cancer [J]. J Korean Med Sci, 1998； 13 (3)： 286-290.
23. Otani Y. The collagenase activities, interstitial collagenase and type Ⅳ collagenase, in human stomach cancer: with special reference to local spreading and lymph node metastasis [J]. Keio J Med, 1990； 39(3): l59-167.
24. Ko K, Yazumi S, Yoshikawa K, et al. Activation of fibroblast-derived matrix metalloproteinase-2 by colon-cancer cells in non-contact Co-cultures [J]. Int J Cancer, 2000； 87(2): 165-171.
25. 劉偉, 安杰, 張超. 尼美舒利對(duì)結(jié)腸腺癌細(xì)胞MMP-2表達(dá)的影響 [J]. 中國(guó)普外基礎(chǔ)與臨床雜志， 2005； 12（6）： 585-588.
  1. 1. Li J, Yen C, Liaw D, et al. PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer [J]. Science, 1997； 275(5308): 1943-1947.
  2. 2. Li DM, Sun H. TEP1, encoded by a candidate tumor suppressor locus, is a novel protein tyrosine phosphatase regulated by transforming growth factor beta [J]. Cancer Res, 1997； 57 (11): 2124-2129.
  3. 3. Steck PA, Pershouse MA, Jasser SA, et al. Identification of a candidate tumor suppressor gene MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers [J]. Nat Genet, 1997； 15(4): 356-362.
  4. 4. 熊敏, 吳一龍主編. 現(xiàn)代肺癌病理與臨床 [M]. 北京： 北京科學(xué)出版社 ，2003: 97-98.
  5. 5. Zhao MD, Hu XM, Sun DJ, et al. Expression of some tumor associated factors in human carcinogenesis and development of gastric carcinoma [J]. World J Gastroenterol, 2005； 11 (21): 3217-3221.
  6. 6. Forsyth PA, Wong H, Laing TD, et al. Gelatinase-A (MMP-2), gelatinase-B (MMP-9) and membrane type matrix metalloproteinase-1 (MT1-MMP) are involved in different aspects of the pathophysiology of malignant gliomas [J]. Br J Cancer, 1999； 79 (11-12): 1828-1835.
  7. 7. Yoo CH, Noh SH, Kim YI, et al. Comparison of prognostic significance of nodal staging between old (4th edition) and new (5th edition) UICC TNM classification for gastric carcinoma. International Union Against Cancer [J]. World J Surg, 1999; 23(5): 492-497.
  8. 8. Jawhari A, Jordan S, Poole S, et al. Abnormal immunoreactivity of the E-cadherin-catenin complex in gastric carcinoma: relationship with patient survival [J]. Gastroenterology， 1997; 112(1): 46-54.
  9. 9. 陳昊, 李玉民, 何明彥, 等. 胃不典型增生和癌組織中Ki67和PTEN蛋白的表達(dá)及意義 [J]. 中國(guó)普外基礎(chǔ)與臨床雜志， 2007； 14（3）： 304-307.
  10. 10. Tamura M, Gu J, Takino T, et al. Tumor suppressor PTEN inhibition of cell invasion, migration and growth: differential involvement of focal adhesion kinase and pl30Cas [J]. Cancer Res, 1999； 59(2): 442-449.
  11. 11. Yang L, Kuang LG, Zheng HC, et al. PTEN encoding product: a marker for tumorigenesis and progression of gastric carcinoma [J]. World J Gastroenterol, 2003； 9(1): 35-39.
  12. 12. Fei G, Ebert MP, Mawrin C, et al. Reduced PTEN expression in gastric cancer and in the gastric mucosa of gastric cancer relatives [J]. Eur J Gastroenterol Hepatol, 2002； 14(3): 297-303.
  13. 13. 李海民, 竇科峰, 李韌, 等. 肝癌組織中Fas和FasL mRNA表達(dá)水平的變化及其意義 [J]. 中國(guó)普外基礎(chǔ)與臨床雜志， 2005； 12（3）： 284-286.
  14. 14. 何貴金, 于發(fā)強(qiáng), 高沁怡, 等. 103鈀放射性支架對(duì)Fas基因表達(dá)影響及與膽管癌細(xì)胞凋亡關(guān)系研究 [J]. 中國(guó)普外基礎(chǔ)與臨床雜志， 2007； 14（3）： 284-286.
  15. 15. Kusakabe T, Nashimoto A, Honma K, et al. Fatty acid synthase is highly expressed in carcinoma, adenoma and in regenerative epithelium and intestinal metaplasia of the stomach [J]. Histopathology, 2002； 40(1): 71-79.
  16. 16. Morimoto Y, Hizuta A, Ding EX, et al. Funtional expression of Fas and Fas ligand on human intestinal intraepithelial lymphocytes [J]. Clin Exp immuno1, 1999； 116(1): 84-89.
  17. 17. 項(xiàng)良光, 林新龍, 偉青, 等. 耐阿霉素肝癌細(xì)胞HepG2表達(dá)Fas的變化 [J]. 中國(guó)普外基礎(chǔ)與臨床雜志， 2000； 7（4）： 229-230.
  18. 18. Elnemr A, Ohta T, Yachie A, et al. Human pancreatic cancer cells disable function of Fas receptors at several levels in Fas signal transduction pathway [J]. Int J Oncol, 2001； 18(2): 311-316.
  19. 19. Nabeshima K, Inoue T, Shimao Y, et al． Front-cells- specific expression of membrane-type 1 matrix metalloproteinase and gelatinase A during cohort migration of colon carcinoma cells induced by hepatocyte growth factor／scatter factor [J]． Cancer Res, 2000； 60(13): 3364-3369.
  20. 20. Grigioni WF, D’Errico A, Fiorentino M, et al. Gelatinase A (MMP-2) and its mRNA detected in both neoplastic and stromal cells of tumors with different invasive and metastatic properties [J]． Diagn Mol Pathol, 1994； 3(3): 163-169.
  21. 21. Nomura H, Fujimoto N, Seiki M, et al. Enhanced production of matrix metalloproteinases and activation of matrix metalloproteinase 2 (gelatinase A) in human gastric carcinomas [J]. Int J Cancer, 1996； 69(1): 9-16.
  22. 22. Ko BK, Cho HR, Choi DW, et al. Reduced expression of tissue inhibitor of metalloproteinase in nodal metastasis of stomach cancer [J]. J Korean Med Sci, 1998； 13 (3)： 286-290.
  23. 23. Otani Y. The collagenase activities, interstitial collagenase and type Ⅳ collagenase, in human stomach cancer: with special reference to local spreading and lymph node metastasis [J]. Keio J Med, 1990； 39(3): l59-167.
  24. 24. Ko K, Yazumi S, Yoshikawa K, et al. Activation of fibroblast-derived matrix metalloproteinase-2 by colon-cancer cells in non-contact Co-cultures [J]. Int J Cancer, 2000； 87(2): 165-171.
  25. 25. 劉偉, 安杰, 張超. 尼美舒利對(duì)結(jié)腸腺癌細(xì)胞MMP-2表達(dá)的影響 [J]. 中國(guó)普外基礎(chǔ)與臨床雜志， 2005； 12（6）： 585-588.