目的 研究P 物質(zhì)( substance P , SP) 對(duì)體外培養(yǎng)的人低分化胃癌細(xì)胞MKN45 內(nèi)游離鈣濃度的影響。方法 在RPMI 1640 培養(yǎng)液中培養(yǎng)人胃癌細(xì)胞系MKN45 ,應(yīng)用鈣特異性熒光指示劑Furu23/ AM 負(fù)載細(xì)胞,用ASN21377642 (N K21 受體拮抗劑) 、尼卡地平和不同濃度的SP 作用于人胃癌細(xì)胞MKN45 ,采用激光掃描共聚焦顯微鏡觀測胃癌細(xì)胞內(nèi)游離鈣濃度的變化。結(jié)果 在Hanks 液(含鈣離子) 中加入10 、50 及100 nmol/ L SP 可以顯著升高胃癌細(xì)胞內(nèi)鈣離子濃度( P lt; 0. 05) ,并且呈劑量依賴性( P lt; 0. 05) ; 在無外鈣時(shí),SP 引起細(xì)胞內(nèi)鈣離子濃度升高的幅度低于有外鈣時(shí)( P lt; 0. 05) ; 在Hanks 液中加入N K21 受體拮抗劑ASN21377642 和鈣通道阻滯劑尼卡地平孵育后,SP 引起胃癌細(xì)胞內(nèi)鈣離子濃度升高的幅度顯著降低,與Hanks 液組比較差異有統(tǒng)計(jì)學(xué)意義( P lt;0. 05) 。結(jié)論 SP 可以顯著升高胃癌細(xì)胞內(nèi)鈣離子濃度,細(xì)胞內(nèi)鈣離子濃度的升高源于內(nèi)鈣釋放和外鈣內(nèi)流。
引用本文： 管臣,張洪偉,王為忠,宋俊峰,豐帆,季剛. P 物質(zhì)對(duì)體外培養(yǎng)的胃癌細(xì)胞內(nèi)游離鈣濃度的影響. 中國普外基礎(chǔ)與臨床雜志, 2008, 15(1): 8-11. doi: 復(fù)制
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- 1. Castagliuolo I,Valenick L,Liu J,et al.Epidermal growth factor receptor transactivation mediates substance P-induced mitogenic responses in U-373 MG cells[J].J Biol Chem,2000; 275(34)∶26545.
- 2. Corbett EF,Michalak M.Calcium,a signaling molecule in the endoplasmic reticulum?[J].Trends Biochem Sci,2000; 25(7)∶307.
- 3. Teruel MN,Chen W,Persechini A,et al.Differential codes for free Ca(2+)-calmodulin signals in nucleus and cytosol[J].Curr Biol,2000; 10(2)∶86.
- 4. 劉君,李寧,宋少柏.胃癌的分子生物學(xué)研究進(jìn)展[J].中國普外基礎(chǔ)與臨床雜志,2000; 7(3)∶197.
- 5. 張多強(qiáng),李玉民,何曉東,等.黏著斑激酶的高表達(dá)與人胃癌侵襲和轉(zhuǎn)移的關(guān)系[J].中國普外基礎(chǔ)與臨床雜志,2006; 13(1)∶43.
- 6. 張洪偉,王為忠,宋俊峰,等.SP免疫陽性神經(jīng)纖維在胃癌組織中分布的實(shí)驗(yàn)研究人[J].中國現(xiàn)代醫(yī)學(xué)雜志,2005; 15(24)∶3692.
- 7. 張洪偉,王為忠,宋俊峰,等.人胃癌組織中神經(jīng)纖維發(fā)生可塑性改變的研究[J].中國癌癥雜志,2007; 17(3)∶261.
- 8. 張洪偉,王為忠,宋俊峰,等.P物質(zhì)對(duì)胃癌細(xì)胞增殖的作用的研究[J].中華實(shí)驗(yàn)外科雜志,2007; 24(6)∶150.
- 9. 薛少白,張鴻卿,王端順,等譯.細(xì)胞增殖的生物學(xué)[M].北京:北京大學(xué)出版社,1985∶99～123.
- 10. Oh EJ,Gover TD,Cordoba-Rodriguez R,et al.Substance P evokes cation currents through TRP channels in HEK293 cells[J].J Neurophysiol,2003; 90(3)∶2069.
- 11. Ito K,Rome C,Bouleau Y,et al.Substance P mobilizes intracellular calcium and activates a nonselective cation conductance in rat spiral ganglion neurons[J].Eur J Neurosci,2002; 16(11)∶2095.
- 12. Sun W,Ding DL,Wang P,et al.Substance P inhibits potassium and calcium currents in inner ear spiral ganglion neurons[J].Brain Res,2004; 1012(1-2)∶82.
- 13. 史娟,李繼碩.TRP離子通道[J].神經(jīng)解剖學(xué)雜志,2004; 20(2)∶197.
- 14. Galiano M,Gasparre G,Lippe C,et al.Calcium response after stimulation by substance P of U373 MG cells:inhibition of store-operated calcium entry by protein kinase C[J].Cell Calcium,2004; 35(2)∶123.