目的 探討TLSFJM(JM急性T淋巴細(xì)胞白血病細(xì)胞分泌的抑制因子)作為抑制因子對小腸移植急性排斥反應(yīng)的抑制效應(yīng)及其機(jī)理,并與FK506的抑制效應(yīng)特點(diǎn)和副作用進(jìn)行比較分析.方法 雄性BN和LEW大鼠分別作為供、受體行小腸移植,共分5組: 小腸移植組(SBT組)、大劑量FK506 [0.5 mg/(kg·d)]組、小劑量FK506 [0.25 mg/(kg·d)]組、TLSFJM [10 U/(kg·d)]組和FK506 [0.25 mg/(kg·d)]+TLSFJM [10 U/(kg·d)]組.FK506和TLSFJM分別以肌肉或腹腔注射方式給藥.在不同時(shí)間段分別觀察各組動(dòng)物體重、生存時(shí)間及肝、腎功能,組織病理學(xué)檢查排斥反應(yīng)發(fā)生情況.結(jié)果 TLSFJM應(yīng)用7 d,對移植大鼠肝、腎功能無損害.TLSFJM+小劑量FK506不但避免了大劑量FK506對肝功能的損害,而且顯著延長了受體的生存時(shí)間.但單獨(dú)應(yīng)用TLSFJM仍有一定程度排斥反應(yīng)出現(xiàn),不能明顯延長受體的生存時(shí)間.結(jié)論 TLSFJM作為一種有效的移植免疫抑制因子,聯(lián)合小劑量FK506應(yīng)用能延長宿主和移植腸的生存時(shí)間,延緩排斥反應(yīng)的發(fā)生,減輕排斥反應(yīng)的強(qiáng)度,避免大劑量FK506治療帶來的并發(fā)癥.TLSFJM有望成為一種新型、高效、低毒的免疫抑制劑.
引用本文： 董光龍,李開宗,王為忠,張章,陳麗華,金伯泉. TLSFJM聯(lián)合小劑量FK506 促進(jìn)大鼠小腸移植耐受. 中國普外基礎(chǔ)與臨床雜志, 2008, 15(1): 41-45. doi: 復(fù)制
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| 2. | Fung JJ,Starzl TE.FK506 in solid organ transplantation[J].Transplant Proc,1994; 26(5)∶3017. |
| 3. | Guo WH,Tian L,Dallmann MJ,et al.Comparative study of allograft survival of heterotopic and orthotopic small bowel transplantation in rat[J].Transplantation,2003; 75(11)∶1895. |
| 4. | Higuchi K,Kimura O,Furukawa T,et al.FK506 induces the atrophy of enteric ganglia in small bowel transplantation,which can be prevented by the neuropeptide bombesin[J].Transplant Proc,2006; 38(6)∶1823. |
| 5. | 董光龍,李開宗,王為忠.雙Cuff雙造口大鼠異位小腸移植模型的建立[J].第四軍醫(yī)大學(xué)學(xué)報(bào),2003; 24(2)∶189. |
| 6. | Grant D.Current results of intestinal transplantation.The International Intestinal Transplant Registry[J].Lancet,1996; 347(9018)∶1801. |
| 7. | 魏曉平,李立.他克莫司與環(huán)孢素A在器官移植中的應(yīng)用比較[J].中國普外基礎(chǔ)與臨床雜志,2003; 10(4)∶415. |
| 8. | Jiang H,Kobayashi M.Differences between cyclosporin A and tacrolimus in organ transplantation[J].Transplant Proc,1999; 31(5)∶1978. |
| 9. | Miyauchi T,Ishikawa M,Tashiro S,et al.Effect of donor-specific splenocytes via portal vein and FK506 in rat small bowel transplantation[J].Transplantation,1998; 65(1)∶27. |
| 10. | 金伯泉.一株人急性淋巴細(xì)胞白血病細(xì)胞所分泌抑制因子的免疫調(diào)節(jié)作用[J].中華血液學(xué)雜志,1990; 11(5)∶247. |
| 11. | 申立中,金伯泉,夏海濱,等.TLSFJM對DNFB誘導(dǎo)的小鼠接觸性皮炎和同種異體皮片移植排斥反應(yīng)的抑制作用[J].細(xì)胞與分子免疫學(xué)雜志,1996; 12(4)∶27. |
| 12. | 韓衛(wèi)寧,李德敏,蘇麗,等.TLSFJM對同種異體抗原誘導(dǎo)的小鼠殺傷性T細(xì)胞分化及效應(yīng)功能的影響[J].細(xì)胞與分子免疫學(xué)雜志,1999; 15(2)∶118. |
| 13. | Guo WH,Tian L,Chan KL,et al.Role of CD4+ and CD8+ T cells in early and late acute rejection of small bowel allograft[J].J Pediatr Surg,2001; 36(2)∶352. |
| 14. | Ma H,Wang J,Wang J,et al.Features of chronic allograft rejection on rat small intestine transplantation[J].Pediatr Transplant,2007; 11(2)∶165. |
| 15. | Song J,Ito T,Matsuda C,et al.Regulation of donor T cells in the tolerant rats to graft-versus-host disease by FTY720 following small bowel transplantation[J].Transplant Proc,2006; 38(10)∶3181. |
| 16. | Gasser M,Otto C,Timmermann W,et al.Putative regulatory function of alloreactive Th2 cells in tolerant recipients after small bowel transplantation[J].Transplant Proc,2001; 33(1-2)∶161. |
| 17. | 智星,嚴(yán)律南,楊培,等.CTLA4-Ig抗大鼠肝移植排斥反應(yīng)及誘導(dǎo)免疫耐受的實(shí)驗(yàn)研究[J].中國普外基礎(chǔ)與臨床雜志,2005; 12(6)∶600. |
| 18. | 董光龍,李開宗,王為忠.TLSFJM對小腸移植大鼠外周血及移植腸浸潤淋巴細(xì)胞的影響[J].免疫學(xué)雜志,2003; 19(1)∶36. |
- 1. <>[1]Tzakis AG,Nery JR,Thompson J,et al.New immunosuppressive regimens in clinical intestinal transplantation[J].Transplant Proc,1997; 29(1-2)∶683.
- 2. Fung JJ,Starzl TE.FK506 in solid organ transplantation[J].Transplant Proc,1994; 26(5)∶3017.
- 3. Guo WH,Tian L,Dallmann MJ,et al.Comparative study of allograft survival of heterotopic and orthotopic small bowel transplantation in rat[J].Transplantation,2003; 75(11)∶1895.
- 4. Higuchi K,Kimura O,Furukawa T,et al.FK506 induces the atrophy of enteric ganglia in small bowel transplantation,which can be prevented by the neuropeptide bombesin[J].Transplant Proc,2006; 38(6)∶1823.
- 5. 董光龍,李開宗,王為忠.雙Cuff雙造口大鼠異位小腸移植模型的建立[J].第四軍醫(yī)大學(xué)學(xué)報(bào),2003; 24(2)∶189.
- 6. Grant D.Current results of intestinal transplantation.The International Intestinal Transplant Registry[J].Lancet,1996; 347(9018)∶1801.
- 7. 魏曉平,李立.他克莫司與環(huán)孢素A在器官移植中的應(yīng)用比較[J].中國普外基礎(chǔ)與臨床雜志,2003; 10(4)∶415.
- 8. Jiang H,Kobayashi M.Differences between cyclosporin A and tacrolimus in organ transplantation[J].Transplant Proc,1999; 31(5)∶1978.
- 9. Miyauchi T,Ishikawa M,Tashiro S,et al.Effect of donor-specific splenocytes via portal vein and FK506 in rat small bowel transplantation[J].Transplantation,1998; 65(1)∶27.
- 10. 金伯泉.一株人急性淋巴細(xì)胞白血病細(xì)胞所分泌抑制因子的免疫調(diào)節(jié)作用[J].中華血液學(xué)雜志,1990; 11(5)∶247.
- 11. 申立中,金伯泉,夏海濱,等.TLSFJM對DNFB誘導(dǎo)的小鼠接觸性皮炎和同種異體皮片移植排斥反應(yīng)的抑制作用[J].細(xì)胞與分子免疫學(xué)雜志,1996; 12(4)∶27.
- 12. 韓衛(wèi)寧,李德敏,蘇麗,等.TLSFJM對同種異體抗原誘導(dǎo)的小鼠殺傷性T細(xì)胞分化及效應(yīng)功能的影響[J].細(xì)胞與分子免疫學(xué)雜志,1999; 15(2)∶118.
- 13. Guo WH,Tian L,Chan KL,et al.Role of CD4+ and CD8+ T cells in early and late acute rejection of small bowel allograft[J].J Pediatr Surg,2001; 36(2)∶352.
- 14. Ma H,Wang J,Wang J,et al.Features of chronic allograft rejection on rat small intestine transplantation[J].Pediatr Transplant,2007; 11(2)∶165.
- 15. Song J,Ito T,Matsuda C,et al.Regulation of donor T cells in the tolerant rats to graft-versus-host disease by FTY720 following small bowel transplantation[J].Transplant Proc,2006; 38(10)∶3181.
- 16. Gasser M,Otto C,Timmermann W,et al.Putative regulatory function of alloreactive Th2 cells in tolerant recipients after small bowel transplantation[J].Transplant Proc,2001; 33(1-2)∶161.
- 17. 智星,嚴(yán)律南,楊培,等.CTLA4-Ig抗大鼠肝移植排斥反應(yīng)及誘導(dǎo)免疫耐受的實(shí)驗(yàn)研究[J].中國普外基礎(chǔ)與臨床雜志,2005; 12(6)∶600.
- 18. 董光龍,李開宗,王為忠.TLSFJM對小腸移植大鼠外周血及移植腸浸潤淋巴細(xì)胞的影響[J].免疫學(xué)雜志,2003; 19(1)∶36.