目的 研究蛋白基因產(chǎn)物9.5（protein gene product 9.5,PGP9.5）在人胃癌組織中的表達(dá)，并探討其與胃腫瘤的發(fā)生、發(fā)展和侵襲、轉(zhuǎn)移的關(guān)系。
方法 采用免疫組織化學(xué)SP法和Western blot法檢測(cè)80例胃癌及8例正常胃組織中PGP9.5的表達(dá)。
結(jié)果 在80例胃癌組織中有56例（70.0%）表達(dá)PGP9.5，而正常胃上皮細(xì)胞中無(wú)PGP9.5表達(dá)； Western blot檢測(cè)結(jié)果與免疫組化結(jié)果一致。SP法顯示PGP9.5的表達(dá)和胃癌的浸潤(rùn)深度、分化程度及有無(wú)淋巴結(jié)轉(zhuǎn)移有關(guān)（P＜0.05），與患者年齡、性別、胃癌的組織學(xué)類型及TNM分期無(wú)關(guān)（P gt;0.05）。
結(jié)論 PGP9.5在胃癌侵襲及轉(zhuǎn)移過程中可能發(fā)揮重要作用，檢測(cè)PGP9.5表達(dá)可作為腫瘤組織侵襲胃的一個(gè)分子標(biāo)志物。
引用本文： 張弦,張小兵,陶凱雄,王國(guó)斌. 人胃癌PGP9.5的表達(dá)及其臨床意義. 中國(guó)普外基礎(chǔ)與臨床雜志, 2007, 14(1): 76-79. doi: 復(fù)制
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| 5. | Takase T, Hibi K, Yamazaki T, et al. PGP9.5 overexpression in esophageal squamous cell carcinoma [Ｊ]. Hepatogastroenterology， 2003; 50(53)∶1278. |
| 6. | Lee YM, Lee JY, Kim MJ, et al. Hypomethylation of the protein gene product 9.5 promoter region in gallbladder cancer and its relationship with clinicopathological features [Ｊ]. Cancer Sci， 2006; 97(11)∶1205. |
| 7. | Yamazaki T, Hibi K, Takase T, et al. PGP9.5 as a marker for invasive colorectal cancer [Ｊ]. Clin Cancer Res， 2002; 8(1)∶192. |
| 8. | Tezel E, Hibi K, Nagasaka T, et al. PGP9.5 as a prognostic factor in pancreatic cancer [Ｊ]. Clin Cancer Res， 2000; 6(12)∶4764. |
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- 1. Otsuki T, Yata K, Takata-Tomokuni A, et al. Expression of protein gene product 9.5 (PGP9.5)/ubiquitin-C-terminal hydrolase 1 (UCHL-1) in human myeloma cells [Ｊ]. Br J Haematol， 2004; 127(3)∶292.
- 2. Mandelker DL, Yamashita K, Tokumaru Y, et al. PGP9.5 promoter methylation is an independent prognostic factor for esophageal squamous cell carcinoma [Ｊ]. Cancer Res， 2005; 65(11)∶4963.
- 3. Hayashi H, Ochiai T, Suzuki T, et al. Superiority of a new AJCC-TNM staging system for gastric carcinoma [Ｊ]. Surgery, 2003; 127(5)∶129.
- 4. Takano T, Miyauchi A, Matsuzuka F, et al. PGP9.5 mRNA could contribute to the molecular-based diagnosis of medullary thyroid carcinoma [Ｊ]. Eur J Cancer， 2004; 40(4)∶614.
- 5. Takase T, Hibi K, Yamazaki T, et al. PGP9.5 overexpression in esophageal squamous cell carcinoma [Ｊ]. Hepatogastroenterology， 2003; 50(53)∶1278.
- 6. Lee YM, Lee JY, Kim MJ, et al. Hypomethylation of the protein gene product 9.5 promoter region in gallbladder cancer and its relationship with clinicopathological features [Ｊ]. Cancer Sci， 2006; 97(11)∶1205.
- 7. Yamazaki T, Hibi K, Takase T, et al. PGP9.5 as a marker for invasive colorectal cancer [Ｊ]. Clin Cancer Res， 2002; 8(1)∶192.
- 8. Tezel E, Hibi K, Nagasaka T, et al. PGP9.5 as a prognostic factor in pancreatic cancer [Ｊ]. Clin Cancer Res， 2000; 6(12)∶4764.
- 9. Yamashita K, Park HL, Kim MS, et al. PGP9.5 methylation in diffuse-type gastric cancer [Ｊ]. Cancer Res， 2006; 66(7)∶3921.