目的 觀察氯化釓(GdCl3)對(duì)內(nèi)毒素刺激后的小鼠巨噬細(xì)胞來(lái)源的細(xì)胞系RAW264.7細(xì)胞Toll樣受體(TLRs)表達(dá)的影響。方法 RAW264.7細(xì)胞分為空白組、內(nèi)毒素處理組（LPS組）及GdCl3處理組（GdCl3組），采用流式細(xì)胞儀檢測(cè)TLR2/4蛋白表達(dá)情況； 用逆轉(zhuǎn)錄PCR（RT-PCR）法分析細(xì)胞中TLR2/4 mRNA表達(dá)的變化； 用ELISA檢測(cè)細(xì)胞培養(yǎng)上清液中TNF-α的水平。結(jié)果 與LPS組相比，不同濃度的GdCl3作用于RAW264.7細(xì)胞后，其TLR2/4蛋白和基因的表達(dá)以及TNF-α的表達(dá)水平均明顯下降，在觀察濃度范圍內(nèi)以2 000 μmol/L時(shí)最明顯，TLR2/4蛋白： 200 μmol/L時(shí)為（70.2±1.28）％/（66.7±2.59）％，400 μmol/L時(shí)為（64.9±1.43）％/（60.4±1.25）％，2 000 μmol/L時(shí)為（47.4±0.98）％/（32.1±0.74）％，其與 LPS組的（94.4±1.76）％/（95.7±0.87）％比較，P＜0.01； TLR2/4 mRNA （A值）： 200 μmol/L時(shí)為（76.42±2.76）/（101.72±3.14）， 400 μmol/L時(shí)為（75.60±3.76）/（89.65±5.17），2 000 μmol/L時(shí)為（64.22±4.67）/（78.44±4.88），其與 LPS組的（127.64±3.25）/（119.82±5.59）比較，P＜0.05, P＜0.01； TNF-α： 200 μmol/L時(shí)為 （2 540±77） pg/ml， 400 μmol/L時(shí)為 （2 041±106） pg/ml，2 000 μmol/L時(shí)為（1 020±220） pg/ml ，其與 LPS組的（4 688±127） pg/ml比較，P＜0.01。結(jié)論 GdCl3能明顯抑制內(nèi)毒素引起的RAW264.7細(xì)胞Toll樣受體的表達(dá)及相應(yīng)炎癥因子的生成。
引用本文： 徐建波,吳河水,張進(jìn)祥,王琳,金欽文,田元,張景輝,張磊. 氯化釓對(duì)內(nèi)毒素刺激后小鼠巨噬細(xì)胞Toll樣受體表達(dá)的影響. 中國(guó)普外基礎(chǔ)與臨床雜志, 2006, 13(6): 681-684. doi: 復(fù)制
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- 2. Wu HS, Zhang JX, Wang L, et al. Toll-like receptor 4 inv-olvement in hepatic ischemia/reperfusion injury in mice [Ｊ]. Hepatobiliary Pancreat Dis Int， 2004; 3(2)∶250.
- 3. Medzhitov R. Toll-like receptors and innate immunity [Ｊ]. Nat Rev Immunol， 2001; 1(2)∶135.
- 4. Schauer RJ, Bilzer M, Kalmuk S, et al. Microcirculatory failure after rat liver transplantation is related to Kupffer cell-derived oxidant stress but not involved in early graft dysfunction [Ｊ]. Transplantation， 2001; 72(10)∶1692.
- 5. Shibuya H, Ohkohchi N, Seya K, et al. Kupffer cells generate superoxide anions and modulate reperfusion injury in rat livers after cold preservation [Ｊ]. Hepatology， 1997； 25(2)∶35.
- 6. Dekai Zhang, Guolong Zhang, Matthew S, et al. A toll-like receptor that prevents infection by uropathogenic bacteria[Ｊ]. Science， 2004; 303（5633）∶1522.
- 7. Aliprantis AO, Yang RB, Mark MR, et al. Cell activation and apoptosis by bacterial lipoproteins through toll-like receptor-2 [Ｊ]. Science， 1999； 285 (5428)∶736.
- 8. 吳河水， 王 峰， 王 琳， 等. 小鼠肝缺血/再灌注損傷時(shí)肝臟Kupffer細(xì)胞中TLR2的表達(dá) [Ｊ]. 中國(guó)普通外科雜志, 2004； 13(8)∶591.
- 9. Musikacharoen T, Matsuguchi T, Kikuchi T, et al. NF-kappa B and STAT5 play important roles in the regulation of mouse Toll-like receptor 2 gene expression [Ｊ]. J Immunol, 2001； 166（7）∶4516.
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- 11. Lee CM, Yeoh GC, Olynyk JK. Differential effects of gadolinium chloride on Kupffer cells in vivo and in vitro [Ｊ]. Int J Biochem Cell Biol， 2004; 36(3)∶481.