• 中國醫(yī)科大學(xué)第一臨床學(xué)院腫瘤科（沈陽110001）;;
  • 通訊作者： 單吉賢;

目的觀察肝細(xì)胞生長因子/離散因子（HGF/SF）在姜黃素誘導(dǎo)的大腸癌細(xì)胞凋亡中的作用。
方法采用MTT法分析姜黃素對大腸癌細(xì)胞的抑制作用； 流式細(xì)胞術(shù)評價(jià)HGF抗凋亡作用。
結(jié)果不同濃度姜黃素作用Caco-2細(xì)胞后發(fā)現(xiàn),只在64 μg/ml濃度發(fā)揮作用，即抑制細(xì)胞增殖； 而同時(shí)加入不同濃度的HGF后可以拮抗姜黃素引起的Caco-2細(xì)胞生長的抑制作用，但HGF發(fā)揮作用無濃度依賴關(guān)系。流式細(xì)胞術(shù)檢測結(jié)果發(fā)現(xiàn)，姜黃素只在64 μg/ml濃度下誘導(dǎo)Caco-2細(xì)胞凋亡，在加入不同濃度HGF后，凋亡明顯減少，但并無劑量依賴關(guān)系。MAPK途徑在HGF拮抗姜黃素誘導(dǎo)的Caco-2細(xì)胞凋亡中的作用發(fā)現(xiàn)，阻斷p42/p44 MAPK和p38 MAPK后，并不影響HGF對Caco-2細(xì)胞凋亡的保護(hù)作用。
結(jié)論HGF拮抗姜黃素誘導(dǎo)的Caco-2細(xì)胞凋亡，MAPK信號傳導(dǎo)途徑可能不參與此過程。

引用本文： 李宏武,單吉賢. HGF/SF對姜黃素誘導(dǎo)大腸癌細(xì)胞凋亡的作用. 中國普外基礎(chǔ)與臨床雜志, 2006, 13(1): 74-76. doi: 復(fù)制

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11. Chen H, Zhang ZS, Zhang YL, et al. Curcumin inhibits cell proliferation by interfering with the cell cycle and inducing apoptosis in colon carcinoma cells [Ｊ]. Anticancer Res， 1999; 19(5A)∶3675.
12. Moragoda L, Jaszewski R, Majumdar AP. Curcumin induced modulation of cell cycle and apoptosis in gastric and colon cancer cells [Ｊ]. Anticancer Res， 2001; 21(2A)∶873.
13. Ishiki Y, Ohnishi H, Muto Y, et al. Direct evidence that hepatocyte growth factor is a hepatotrophic factor for liver regeneration and has a potent antihepatitis effect in vivo [Ｊ]. Hepatology， 1992; 16(5)∶1227.
14. Xiao GH, Jeffers M, Bellacosa A, et al. Antiapoptotic signaling by hepatocyte growth factor/Met via the phosphatidylinositol 3kinase/Akt and mitogenactivated protein kinase pathways [Ｊ]. Proc Natl Acad Sci USA， 2001; 98(1)∶247.
  1. 1. Montesano R, Matsumoto K, Nakamura T, et al. Identification of a fibroblastderived epithelial morphogen as hepatocyte growth factor [Ｊ]. Cell， 1991; 67(5)∶901.
  2. 2. Nakamura T, Nishizawa T, Hagiya M, et al. Molecular cloning and expression of human hepatocyte growth factor [Ｊ]. Nature， 1989; 342(6248)∶440.
  3. 3. Rubin JS, Bottaro DP, Aaronson SA. Hepatocyte growth factor/scatter factor and its receptor, the cmet protooncogene product [Ｊ]. Biochim Biophys Acta， 1993; 1155(3)∶357.
  4. 4. Stoker M, Gherardi E, Perryman M, et al. Scatter factor is a fibroblastderived modulator of epithelial cell mobility [Ｊ]. Nature， 1987; 327(6119)∶239.
  5. 5. Jeffers M, Rong S, Vande Woude GF. Enhanced tumorigenicity and invasionmetastasis by hepatocyte growth factor/scatter factormet signalling in human cells concomitant with induction of the urokinase proteolysis network [Ｊ]. Mol Cell Biol， 1996; 16(3)∶1115.
  6. 6. Weidner KM, Di Cesare S, Sachs M, et al. Interaction between Gab1 and the cMet receptor tyrosine kinase is responsible for epithelial morphogenesis [Ｊ]. Nature， 1996; 384(6605)∶173.
  7. 7. Kosai K, Matsumoto K, Nagata S, et al. Abrogation of Fasinduced fulminant hepatic failure in mice by hepatocyte growth factor [Ｊ]. Biochem Biophys Res Commun， 1998; 244(3)∶683.
  8. 8. Huang TS, Lee SC, Lin JK. Suppression of cJun/AP1 activation by an inhibitor of tumor promotion in mouse fibroblast cells [Ｊ]. Proc Natl Acad Sci USA， 1991; 88(12)∶5292.
  9. 9. Samaha HS, Kelloff GJ, Steele V, et al. Modulation of apoptosis by sulindac, curcumin, phenylethyl3methylcaffeate, and 6phenylhexyl isothiocyanate: apoptotic index as a biomarker in colon cancer chemoprevention and promotion [Ｊ]. Cancer Res， 1997; 57(7)∶1301.
  10. 10. Huang MT, Lou YR, Xie JG, et al. Effect of dietary curcumin and dibenzoylmethane on formation of 7,12dimethylbenz [a] anthraceneinduced mammary tumors and lymphomas/leukemias in Sencar mice [Ｊ]. Carcinogenesis， 1998; 19(9)∶1697.
  11. 11. Chen H, Zhang ZS, Zhang YL, et al. Curcumin inhibits cell proliferation by interfering with the cell cycle and inducing apoptosis in colon carcinoma cells [Ｊ]. Anticancer Res， 1999; 19(5A)∶3675.
  12. 12. Moragoda L, Jaszewski R, Majumdar AP. Curcumin induced modulation of cell cycle and apoptosis in gastric and colon cancer cells [Ｊ]. Anticancer Res， 2001; 21(2A)∶873.
  13. 13. Ishiki Y, Ohnishi H, Muto Y, et al. Direct evidence that hepatocyte growth factor is a hepatotrophic factor for liver regeneration and has a potent antihepatitis effect in vivo [Ｊ]. Hepatology， 1992; 16(5)∶1227.
  14. 14. Xiao GH, Jeffers M, Bellacosa A, et al. Antiapoptotic signaling by hepatocyte growth factor/Met via the phosphatidylinositol 3kinase/Akt and mitogenactivated protein kinase pathways [Ｊ]. Proc Natl Acad Sci USA， 2001; 98(1)∶247.