【摘要】目的 探討雌激素水平對(duì)三苯氧胺（tamoxifen, TAM）抑制乳腺癌細(xì)胞MCF7（ER陽(yáng)性）生長(zhǎng)的影響。 方法 應(yīng)用不同濃度的雌二醇（estradiol, E2）和10 μmol/L濃度的TAM作用于MCF7細(xì)胞，應(yīng)用四甲基偶氮唑藍(lán)（MTT）比色法分析其對(duì)細(xì)胞生長(zhǎng)的抑制作用。 結(jié)果 用1×10-12 mol/L的 E2開(kāi)始刺激MCF7細(xì)胞生長(zhǎng)，到1×10-9 mol/L時(shí)細(xì)胞生長(zhǎng)達(dá)到平臺(tái)期。10 μmol/L的TAM可以明顯抑制細(xì)胞生長(zhǎng)，不同濃度的E2可以影響TAM對(duì)MCF7細(xì)胞生長(zhǎng)的抑制作用。當(dāng)E2濃度達(dá)到1×10-8 mol/L 時(shí)，TAM對(duì)細(xì)胞生長(zhǎng)的抑制作用最弱。 結(jié)論 E2是乳腺癌的危險(xiǎn)因素，腫瘤細(xì)胞周?chē)鶨2的濃度可以影響TAM的療效。
引用本文： 韓明強(qiáng),黃韜,田元. 雌激素水平對(duì)三苯氧胺抑制乳腺癌細(xì)胞的體外研究. 中國(guó)普外基礎(chǔ)與臨床雜志, 2005, 12(6): 589-590下轉(zhuǎn)594. doi: 復(fù)制
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