• 1. 四川省樂山市人民醫(yī)院外二科（樂山614000）;;
  • 2. 重慶醫(yī)科大學(xué)附屬一院血管外科（重慶400016）;

【摘要】目的 探討低強度微波（LIM）輻射對血管再狹窄病理過程的影響，以評價LIM輻射防治再狹窄的可能性。 方法 健康雄性新西蘭大白兔48只，隨機分為2組。應(yīng)用Fogarty導(dǎo)管損傷兔髂外動脈（EIA）內(nèi)膜，建立再狹窄動物模型。輻射組采用2 450 MHz微波，功率分別為2、5和10 mW/cm2，局部輻射EIA（1 h/d）。分別于術(shù)后3、7、14及28 d 取材。采用HE和EF染色，觀察EIA形態(tài)學(xué)改變及內(nèi)膜面積、中膜面積及管腔狹窄率； TUNEL標記法檢測細胞凋亡； TEM觀察血管平滑肌細胞（VSMC）表型及超微結(jié)構(gòu)。 結(jié)果 LIM微波輻射后，再狹窄早期炎癥反應(yīng)受到抑制，附壁血栓減少，各時間點VSMC的增殖、遷移被抑制，內(nèi)膜面積和管腔狹窄率顯著低于對照組(P＜0.01)。 細胞凋亡率在術(shù)后3 d各組間差異無統(tǒng)計學(xué)意義（P gt;0.05），其余時間點，輻射組顯著高于對照組(P＜0.01)，其中5 mW/cm2者細胞凋亡率明顯高于其他各組(P＜0.01)。TEM觀察到對照組大量合成型VSMC術(shù)后14 d達（93.50+3.45）%，輻射組VSMC以收縮型為主，大量成纖維細胞和VSMC呈凋亡的形態(tài)學(xué)改變。

結(jié)論 LIM輻射能顯著抑制再狹窄病理過程中的早期炎癥反應(yīng)和VSMC的增殖、遷移，促進細胞凋亡，有效抑制再狹窄的發(fā)生、發(fā)展。LIM輻射存在量效關(guān)系和功率窗效應(yīng)，功率強度為5 mW/cm2時對再狹窄的效應(yīng)最強。

引用本文： 王志剛,雷澤華,時德,喬正榮. 低強度微波輻射對兔髂外動脈損傷后再狹窄的影響. 中國普外基礎(chǔ)與臨床雜志, 2005, 12(6): 595-599. doi: 復(fù)制

1. 1 Ferns GA, Avades TY. The mechanisms of coronary restenosis: insights from experimental models [Ｊ]. Int J Exp Pathol， 2000; 81(2)632 Kolosova LI, Akoev GN, Avelev VD, et al. Effect of lowintensity millimeter wave electromagnetic radiation on regeneration of the sciatic nerve in rats [Ｊ]. Bioelectromagnetics， 1996; 17(1)443 杜忠民，郭鷂. 毫米波輻射對小鼠EMT6乳腺癌細胞增殖活性的影響 [Ｊ]. 中國病理生理雜志， 1994; 10（1）404 王保義，王長廣，王登本,等. 低強度微波輻射對人精子非熱生物效應(yīng)的研究 [Ｊ]. 生物物理學(xué)報，1996; 12（1）1395 Lentz RR, Stensaas LJ, Partlow LM. Effects of millimeterwave radiation on monolayer cell cultures [Ｊ]. J microwave Power, 1990; 15(4)1136 Nossol B, Buse G, Silny J. Influence of weak static and 50 Hz magnetic fields on the redox activity of cytochromeC oxidase [Ｊ]. Bioelectromagnetics, 1993; 14(4)3617 Wen JK, Shi Y. Progress in the study of mechanisms regulating vascular smooth muscle cell proliferation [Ｊ]. Sheng Li Ke Xue Jin Zhan, 1996; 27(2)1498 Peinnequin A, Piriou A, Mathieu J, et al. Nonthermal effects of continuous 2.45 GHz microwaves on Fasinduced apoptosis in human Jurkat Tcell line [Ｊ]. Bioelectrochemistry, 2000; 51(2)1579 Lai H, Singh NP. Acute lowintensity microwave exposure increases DNA singlestrand breaks in rat brain cells [Ｊ]. Bioelectromagnetics, 1995; 16(3)20710 Singh N, Rudra N, Bansal P, et al. Poly ADP ribosylation as a possible mechanism of microwave——biointeraction [Ｊ]. Indian J Physiol Pharmacol， 1994; 38(3)18111 Lai H, Singh NP. Melatonin and a spintrap compound block radiofrequency electromagnetic radiationinduced DNA strand breaks in rat brain cells [Ｊ]. Bioelectromagnetics， 1997; 18(6)44612 Harvey C, French PW. Effects on protein kinase C and gene expression in a human mast cell line, HMC1, following microwave exposure [Ｊ]. Cell Biol Int， 2000; 23(11)739.
  1. 1. 1 Ferns GA, Avades TY. The mechanisms of coronary restenosis: insights from experimental models [Ｊ]. Int J Exp Pathol， 2000; 81(2)632 Kolosova LI, Akoev GN, Avelev VD, et al. Effect of lowintensity millimeter wave electromagnetic radiation on regeneration of the sciatic nerve in rats [Ｊ]. Bioelectromagnetics， 1996; 17(1)443 杜忠民，郭鷂. 毫米波輻射對小鼠EMT6乳腺癌細胞增殖活性的影響 [Ｊ]. 中國病理生理雜志， 1994; 10（1）404 王保義，王長廣，王登本,等. 低強度微波輻射對人精子非熱生物效應(yīng)的研究 [Ｊ]. 生物物理學(xué)報，1996; 12（1）1395 Lentz RR, Stensaas LJ, Partlow LM. Effects of millimeterwave radiation on monolayer cell cultures [Ｊ]. J microwave Power, 1990; 15(4)1136 Nossol B, Buse G, Silny J. Influence of weak static and 50 Hz magnetic fields on the redox activity of cytochromeC oxidase [Ｊ]. Bioelectromagnetics, 1993; 14(4)3617 Wen JK, Shi Y. Progress in the study of mechanisms regulating vascular smooth muscle cell proliferation [Ｊ]. Sheng Li Ke Xue Jin Zhan, 1996; 27(2)1498 Peinnequin A, Piriou A, Mathieu J, et al. Nonthermal effects of continuous 2.45 GHz microwaves on Fasinduced apoptosis in human Jurkat Tcell line [Ｊ]. Bioelectrochemistry, 2000; 51(2)1579 Lai H, Singh NP. Acute lowintensity microwave exposure increases DNA singlestrand breaks in rat brain cells [Ｊ]. Bioelectromagnetics, 1995; 16(3)20710 Singh N, Rudra N, Bansal P, et al. Poly ADP ribosylation as a possible mechanism of microwave——biointeraction [Ｊ]. Indian J Physiol Pharmacol， 1994; 38(3)18111 Lai H, Singh NP. Melatonin and a spintrap compound block radiofrequency electromagnetic radiationinduced DNA strand breaks in rat brain cells [Ｊ]. Bioelectromagnetics， 1997; 18(6)44612 Harvey C, French PW. Effects on protein kinase C and gene expression in a human mast cell line, HMC1, following microwave exposure [Ｊ]. Cell Biol Int， 2000; 23(11)739.