目的建立一種穩(wěn)定的急性壞死性胰腺炎模型。方法36只雄性ICR小鼠用查隨機數(shù)字表的方法隨機分為對照組（n=6）和實驗組（n=30）。實驗組予以雨蛙素腹腔注射（50 μg/kg），每小時注射1次，共7次，并分別于首次注射后9 h、18 h、24 h、48 h和72 h各處死6只； 對照組予以腹腔注射等量的生理鹽水，于注射后18 h處死。比較兩組動物的血淀粉酶值、胰腺重量及胰腺組織學(xué)評分分值。結(jié)果腹腔注射雨蛙素后9 h，小鼠血淀粉酶值升高，胰腺重量增加，18 h達(dá)峰值，24 h后逐漸下降。二者與胰腺病理損害程度相一致。18 h胰腺間質(zhì)炎性細(xì)胞浸潤和腺泡細(xì)胞壞死明顯，胰腺組織學(xué)評分值達(dá)到最大值（P＜0.05）。結(jié)論大劑量雨蛙素腹腔注射能誘導(dǎo)ICR小鼠產(chǎn)生急性壞死性胰腺炎。該模型制備方法簡單，無創(chuàng)傷性，模型穩(wěn)定，重復(fù)性好。
引用本文： 李全生,陳曉理,周總光,劉續(xù)寶,張肇達(dá). 雨蛙素腹腔注射法建立小鼠急性壞死性胰腺炎模型的實驗研究. 中國普外基礎(chǔ)與臨床雜志, 2004, 11(4): 335-337. doi: 復(fù)制
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- 1. Niederau C, Ferrell LD, Grendell JH. Caeruleininduced acute necrotizing pancreatitis in mice: protective effects of proglumide, benzotript, and secretin [Ｊ]. Gastroenterology， 1985; 88(5 Pt 1)∶1192.
- 2. van Laethem JL, Eskinazi R, Louis H, et al. Multisystemic production of interleukin 10 limits the severity of acute pancreatitis in mice [Ｊ]. Gut, 1998; 43(3)∶408.
- 3. 劉興，陳懷仁，楊得同，等. 蛙皮素致小鼠急性壞死性胰腺炎模型 [Ｊ]. 南京鐵道醫(yī)學(xué)院學(xué)報，1996； 15（4）∶286.
- 4. 薛建國，王宇，許元弟. 建立大鼠急性出血壞死性胰腺炎模型方法的改進 [Ｊ]. 中華實驗外科雜志， 1994； 11（5）∶313.
- 5. 趙慧業(yè)，肖國衡，熊啟達(dá)，等. 胰實質(zhì)內(nèi)注射牛黃膽酸鈉引起實驗性大鼠急性出血性胰腺炎模型及其早期損害的研究 [Ｊ]. 中國病理生理雜志， 1986； 2（2）∶112.
- 6. Lombardi B, Estes LW, Longnecker DS. Acute hemorrhagic pancreatitis (massive necrosis) with fat necrosis induced in mice by DLethionine fed with a cholinedeficient diet [Ｊ]. Am J Pathol, 1975; 79(3)∶465.
- 7. Tani S, Itoh H, Okabayashi Y, et al. New model of acute necrotizing pancreatitis induced by excessive doses of arginine in rats[Ｊ]. Dig Dis Sci, 1990; 35(3)∶367.
- 8. Vasilescu C, Herlea V, Buttenschoen K, et al. Endotoxin translocation in two models of experimental acute pancreatitis [Ｊ]. J Cell Mol Med, 2003; 7(4)∶417.
- 9. Watanabe O, Baccino FM, Steer ML, et al. Supramaximal caerulein stimulation and ultrastructure of rat pancreatic acinar cell: early morphological changes during development of experimental pancreatitis [Ｊ]. Am J Physiol, 1984; 246(4 Pt 1)∶G457.
- 10. Tani S, Otsuki M, Itoh H,et al. Histologic and biochemical alterations in experimental acute pancreatitis induced by supramaximal caerulein stimulation [Ｊ]. Int J Pancreatol, 1987; 2(5-6)∶337.