目的 研究大鼠甲狀旁腺細(xì)胞經(jīng)培養(yǎng)后再移植對(duì)其存活的影響。
方法 將經(jīng)膠原酶和胰蛋白酶消化的甲狀旁腺細(xì)胞培養(yǎng)后再行移植，觀察移植物的存活情況，并對(duì)移植物做透射電鏡觀察。
結(jié)果 新鮮甲狀旁腺移植組平均存活期為(9.25±3.45) d； 甲狀旁腺細(xì)胞培養(yǎng)后移植，移植物的存活時(shí)間為(46.25±7.44) d，明顯延長(zhǎng) (P＜0.01)，在50 d觀察期內(nèi)，8只鼠中有6只的血清鈣及PTH值持續(xù)在正常范圍內(nèi)。移植物內(nèi)可見完整的甲狀旁腺細(xì)胞，其內(nèi)見豐富的粗面內(nèi)質(zhì)網(wǎng)、桿狀的線粒體及分泌顆粒。
結(jié)論 大鼠甲狀旁腺細(xì)胞經(jīng)培養(yǎng)后再移植可以延長(zhǎng)移植物的存活時(shí)間，是治療甲狀旁腺功能低下癥的一條有效途徑。
引用本文： 段秀慶,宋純,王江濱,石于波,許評(píng),宋春芳. 大鼠甲狀旁腺細(xì)胞經(jīng)培養(yǎng)后再移植的實(shí)驗(yàn)研究. 中國普外基礎(chǔ)與臨床雜志, 2004, 11(6): 486-488. doi: 復(fù)制
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