• 1.解放軍第四六一醫(yī)院普外科(長春130021);;
  • 2.吉林大學基礎醫(yī)學部第三綜合實驗室(長春130021);;
  • 3.吉林大學公共衛(wèi)生學院衛(wèi)生統(tǒng)計學教研室;;
  • 4.吉林大學中日聯(lián)誼醫(yī)院基本外科;

目的  研究碘與自身免疫性甲狀腺炎(EAT)的關系。
方法  用碘和甲狀腺球蛋白(TG)誘導SD大鼠建立甲狀腺炎模型,隨機分為5組,每組10只。正常對照組(NC組); 少量碘組(LI組): 飲500 μg/L碘化鈉水; 大量碘組(HI組): 飲500 mg/L碘化鈉水; TG組: 于實驗第15天大鼠接受皮內多點注射完全福氏佐劑乳化的TG各100 μg作為初次免疫,2周后以不完全福氏佐劑乳化的TG加強免疫,以后每周加強免疫1次至第6周末實驗結束; TG+HI組: TG及HI給藥方法同上。至第6周末實驗結束處死所有動物,用放免法檢測血清TG抗體(TGAb)及甲狀腺過氧化物酶抗體(TPOAb)效價,并于光鏡下觀察甲狀腺組織淋巴細胞浸潤程度。
結果  TG+HI組、HI組及TG組甲狀腺濾泡破壞及淋巴細胞浸潤均較明顯,TG+HI組及HI組與NC組比較,P<0.05,TG組與NC組比較有增高趨勢,但無統(tǒng)計學意義。3組TGAb、TPOAb均明顯高于NC組及LI組(P<0.01; 僅HI組TPOAb P<0.05)。各組TGAb及TPOAb水平與甲狀腺病理分級呈正相關關系(r=0.9,P<0.05)。
結論  高碘可誘發(fā)SD鼠EAT發(fā)生,TG與高碘結合誘導SD大鼠EAT的作用更明顯。

引用本文: 高福智,耿志輝,陳小梅,嚴京哲,張德恒,鄭澤霖. 碘和甲狀腺球蛋白誘導大鼠自身免疫性甲狀腺炎的研究. 中國普外基礎與臨床雜志, 2004, 11(6): 489-492. doi: 復制

版權信息: ?四川大學華西醫(yī)院華西期刊社《中國普外基礎與臨床雜志》版權所有,未經授權不得轉載、改編

1. Li M, Eastman CJ, Boyages SC. Iodine induced lymphocytic thyroiditis in the BB/W rat: early and late immune phenomena [J]. Autoimmunity, 1993; 14(3)∶181.
2. Allen EM, Appel MC, Braverman LE. The effect of iodide ingestion on the development of spontaneous lymphocytic thyroiditis in the diabetesprone BB/W rat [J]. Endocrinology, 1986; 118(5)∶1977.
3. Tomer Y. Antithyroglobulin autoantibodies in autoimmune thyroid diseases: crossreactive or pathogenic? [J]. Clin Immunol Immunopathol, 1997; 82(1)∶3.
4. 朱惠民,王風榮,尹桂山,等.高碘地方性甲狀腺腫發(fā)病機理的實驗研究 [J]. 中國地方病學雜志, 1989; 8(4)∶220.
5. Rasooly L, Burek CL, Rose NR. Iodineinduced autoimmune thyroiditis in NODH2h4 mice [J]. Clin Immunol Immunopathol, 1996; 81(3)∶287.
6. 孫崴,宋光華,賀斌. 碘和甲狀腺球蛋白誘導大鼠實驗性自身免疫性甲狀腺炎的研究 [J]. 中華內科雜志, 2000; 39(12)∶841.
7. 戴藹善,馬冰彬,陳恒,等. 自體免疫甲狀腺炎在上海地區(qū)的人群患病情況及其診斷標準探討 [J]. 中華內分泌代謝雜志, 1989; 5(2)∶67.
8. 王聆,鄭五飛. ELISA測定TMAb和TGAb的臨床意義 [J]. 中華內分泌代謝雜志, 1989; 5(2)∶70.
9. 田恩江,張佳麗,趙樹君,等. 碘對誘發(fā)易感和非易感小鼠自體免疫甲狀腺炎的實驗研究 [J]. 中國地方病學雜志, 2003; 1(1)∶5.
10. Rodien P, Madec AM, Ruf J, et al. Antibodydependent cellmediated cytotoxicity in autoimmune thyroid disease: relationship to antithyroperoxidase antibodies [J]. J Clin Endocrinol Metab, 1996; 81(7)∶2595.
11. Harach HR, Escalante DA, Onativia A, et al. Thyroid carcinoma and thyroiditis in an endemic goitre region before and after iodine prophylaxis [J]. Acta Endocrinol (Copenh), 1985; 108(1)∶55.
12. 房維堂,王金彪,李平春,等. 引高碘水居民甲狀腺功能和甲狀腺的自身免疫狀況 [J]. 中國地方病雜志, 1994; 3(1)∶9.
13. Park YK, Harland BF, Vanderveen JE, et al. Estimation of dietary iodine intake of Americans in recent years [J]. J Am Diet Assoc, 1981; 79(1)∶17.
14. Safran M, Paul TL, Roti E, et al. Environmental factors affecting autoimmune thyroid disease [J]. Endocrinol Metab Clin North Am, 1987; 16(2)∶327.
15. Ahmann AJ, Burman KD. The role of T lymphocytes in autoimmune thyroid disease [J]. Endocrinol Metab Clin North Am, 1987; 16(2)∶287.
16. 龍振洲. 醫(yī)學免疫學 [M]. 北京: 人民衛(wèi)生出版社,1995∶53~63.
17. Many MC, Papadopoulos J, Martin C, et al. Iodine induced damage in mouse hyperlastic thyroid is associated to lipid peroxidation. In: Gordon Gross & Hennemann. eds. Progress in Thyroid Research [M]. Rotterdam: Balkema 1991∶635-637.
18. Bagchi N, Brown TR, Urdanivia E, et al. Induction of autoimmune thyroiditis in chickens by dietary iodine [J]. Science, 1985; 230(4723)∶325.
  1. 1. Li M, Eastman CJ, Boyages SC. Iodine induced lymphocytic thyroiditis in the BB/W rat: early and late immune phenomena [J]. Autoimmunity, 1993; 14(3)∶181.
  2. 2. Allen EM, Appel MC, Braverman LE. The effect of iodide ingestion on the development of spontaneous lymphocytic thyroiditis in the diabetesprone BB/W rat [J]. Endocrinology, 1986; 118(5)∶1977.
  3. 3. Tomer Y. Antithyroglobulin autoantibodies in autoimmune thyroid diseases: crossreactive or pathogenic? [J]. Clin Immunol Immunopathol, 1997; 82(1)∶3.
  4. 4. 朱惠民,王風榮,尹桂山,等.高碘地方性甲狀腺腫發(fā)病機理的實驗研究 [J]. 中國地方病學雜志, 1989; 8(4)∶220.
  5. 5. Rasooly L, Burek CL, Rose NR. Iodineinduced autoimmune thyroiditis in NODH2h4 mice [J]. Clin Immunol Immunopathol, 1996; 81(3)∶287.
  6. 6. 孫崴,宋光華,賀斌. 碘和甲狀腺球蛋白誘導大鼠實驗性自身免疫性甲狀腺炎的研究 [J]. 中華內科雜志, 2000; 39(12)∶841.
  7. 7. 戴藹善,馬冰彬,陳恒,等. 自體免疫甲狀腺炎在上海地區(qū)的人群患病情況及其診斷標準探討 [J]. 中華內分泌代謝雜志, 1989; 5(2)∶67.
  8. 8. 王聆,鄭五飛. ELISA測定TMAb和TGAb的臨床意義 [J]. 中華內分泌代謝雜志, 1989; 5(2)∶70.
  9. 9. 田恩江,張佳麗,趙樹君,等. 碘對誘發(fā)易感和非易感小鼠自體免疫甲狀腺炎的實驗研究 [J]. 中國地方病學雜志, 2003; 1(1)∶5.
  10. 10. Rodien P, Madec AM, Ruf J, et al. Antibodydependent cellmediated cytotoxicity in autoimmune thyroid disease: relationship to antithyroperoxidase antibodies [J]. J Clin Endocrinol Metab, 1996; 81(7)∶2595.
  11. 11. Harach HR, Escalante DA, Onativia A, et al. Thyroid carcinoma and thyroiditis in an endemic goitre region before and after iodine prophylaxis [J]. Acta Endocrinol (Copenh), 1985; 108(1)∶55.
  12. 12. 房維堂,王金彪,李平春,等. 引高碘水居民甲狀腺功能和甲狀腺的自身免疫狀況 [J]. 中國地方病雜志, 1994; 3(1)∶9.
  13. 13. Park YK, Harland BF, Vanderveen JE, et al. Estimation of dietary iodine intake of Americans in recent years [J]. J Am Diet Assoc, 1981; 79(1)∶17.
  14. 14. Safran M, Paul TL, Roti E, et al. Environmental factors affecting autoimmune thyroid disease [J]. Endocrinol Metab Clin North Am, 1987; 16(2)∶327.
  15. 15. Ahmann AJ, Burman KD. The role of T lymphocytes in autoimmune thyroid disease [J]. Endocrinol Metab Clin North Am, 1987; 16(2)∶287.
  16. 16. 龍振洲. 醫(yī)學免疫學 [M]. 北京: 人民衛(wèi)生出版社,1995∶53~63.
  17. 17. Many MC, Papadopoulos J, Martin C, et al. Iodine induced damage in mouse hyperlastic thyroid is associated to lipid peroxidation. In: Gordon Gross & Hennemann. eds. Progress in Thyroid Research [M]. Rotterdam: Balkema 1991∶635-637.
  18. 18. Bagchi N, Brown TR, Urdanivia E, et al. Induction of autoimmune thyroiditis in chickens by dietary iodine [J]. Science, 1985; 230(4723)∶325.