• 中國醫(yī)學(xué)科學(xué)院中國協(xié)和醫(yī)科大學(xué)北京協(xié)和醫(yī)院外科(北京100730);

目的通過檢測(cè)腫瘤壞死因子相關(guān)細(xì)胞凋亡誘導(dǎo)配體受體-4（TRAIL-R4）在胰腺癌組織中的表達(dá)，探討胰腺癌細(xì)胞抵抗細(xì)胞凋亡的機(jī)理。方法應(yīng)用mRNA印跡法（Northern blotting）、蛋白質(zhì)印跡法（Western blotting）和免疫組織化學(xué)技術(shù)，定性、定位分析TRAIL-R4在正常胰腺組織和胰腺癌組織中的表達(dá)。結(jié)果TRAIL-R4 mRNA和蛋白在正常胰腺組織中呈弱表達(dá)或不表達(dá)，而在胰腺癌組織中呈高表達(dá)； 免疫組織化學(xué)檢測(cè)顯示，在胰腺癌細(xì)胞中TRAIL-R4蛋白呈強(qiáng)染色。結(jié)論TRAIL-R4表達(dá)水平在正常胰腺組織與胰腺癌組織中存在顯著差異，提示胰腺癌細(xì)胞中可能存在對(duì)TRAIL介導(dǎo)的細(xì)胞凋亡抵抗新機(jī)理。

引用本文： 廖泉,郭俊超,趙玉沛. 腫瘤壞死因子相關(guān)細(xì)胞凋亡誘導(dǎo)配體受體-4在人胰腺癌組織中的表達(dá). 中國普外基礎(chǔ)與臨床雜志, 2003, 10(6): 535-538. doi: 復(fù)制

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  2. 2. Ozawa F, Friess H, Kleeff J, et al. Effects and expression of TRAIL and its apoptosispromoting receptors in human pancreatic cancer [Ｊ]. Cancer Lett, 2001; 163(1)∶71.
  3. 3. Walczak H, Miller RE, Ariail K, et al. Tumoricidal activity of tumor necrosis factorrelated apoptosisinducing ligand in vivo [Ｊ]. Nat Med, 1999; 5(2)∶157.
  4. 4. Pan G, Ni J, Yu GL, et al. TRUNDD, a new member of the TRAIL receptor family that antagonizes TRAIL signaling [Ｊ]. FEBS Letter, 1998; 424(1)∶41.
  5. 5. DegliEsposti MA, Dougall WC, Smolak PJ, et al. The novel receptor TRAILR4 induces NFκB and protects against TRAILmediated apoptosis, yet retains an incomplete death domain [Ｊ]. Immunity, 1997; 7(4)∶813.
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