• 1.西安交通大學(xué)第二醫(yī)院普外科（西安710004）;;
  • 2.新疆醫(yī)科大學(xué)第一附屬醫(yī)院普外科（烏魯木齊830000）;

目的 探討慢性胰腺炎動物模型的制作方法及各類模型的特點(diǎn)。 方法 通過對近年來慢性胰腺炎動物模型的相關(guān)文獻(xiàn)的回顧，總結(jié)各模型的制作方法、特點(diǎn)及適合研究各種慢性胰腺炎的最佳模型。 結(jié)果 飲食、胰管結(jié)扎、蛙皮素、二丁基二氯化物（DBTC）、動脈注射微球、動脈結(jié)扎及胰管注射等方法可誘導(dǎo)不同的慢性胰腺炎動物模型。飲食法誘發(fā)自發(fā)性慢性胰腺炎，胰管結(jié)扎法和胰管注射法誘導(dǎo)慢性阻塞性胰腺炎，蛙皮素誘導(dǎo)復(fù)發(fā)性慢性胰腺炎，動脈注射微球和動脈結(jié)扎誘導(dǎo)慢性活動性胰腺炎。結(jié)論 不同的方法均可誘導(dǎo)慢性胰腺炎模型，但各具特點(diǎn)。

引用本文： 陳啟龍,溫浩,李國威. 慢性胰腺炎動物模型研究進(jìn)展. 中國普外基礎(chǔ)與臨床雜志, 2003, 10(6): 560-563. doi: 復(fù)制

1. Etemad B, Whitcomb DC. Chronic pancreatitis: diagnosis, classification, and new genetic developments [J]. Gastroenterology, 2001; 120(3)∶682.
2. 佟衛(wèi)剛，盧實(shí)春，胡驥，等. 慢性胰腺炎的外科治療及遠(yuǎn)期療效 [J]. 中國普外基礎(chǔ)與臨床雜志, 1999; 6(6)∶347.
3. Ohashi K, Kim JH, Hara H, et al. WBN/Kob rats. A new spontaneously occurring model of chronic pancreatitis [J]. Int J pancreatol, 1990; 6(4)∶231.
4. Su SB, Motoo Y, Xie MJ, et al. Expression of transforming growth factorbeta in spontaneous chronic pancreatitis in the WBN/Kob rat [J]. Dig Dis Sci, 2000; 45(1)∶151.
5. 耿智敏，向國安，韓慶，等. 轉(zhuǎn)化生長因子β1在膽管愈合過程中的表達(dá)及意義 [J]. 中國普外基礎(chǔ)與臨床雜志, 2000; 7(6)∶362.
6. Su SB, Motoo Y, Iovanna JL, et al. Effect of camostat mesilate on the expression of pancreatitisassociated protein (PAP), p8, and cytokines in rat spontaneous chronic pancreatitis [J]. Pancreas, 2001; 23(2)∶134.
7. Su SB, Motoo Y, Xie MJ, et al. Apoptosis in rat spontaneous chronic pancreatis: role of the Fas and Fas ligand system [J]. Dig Dis Sci, 2001; 46(1)∶166.
8. Widdison AL, Alvarez C, Schwarz M, et al. The influence of ethanol on pancreatic blood flow in cats with chronic pancreatitis [J]. Surgery, 1992; 112(2)∶202.
9. Karanjia ND, Widdison AL, Leung F, et al. Compartment syndrome in experimental chronic obstructive pancreatitis: effect of decompressing the main pancreatic duct [J]. Br J Surg, 1994; 81(2)∶ 259.
10. Reber PU, Patel AG, Toyama MT, et al. Feline model of chronic obstructive pancreatitis: effects of acute pancreatic duct decompression on blood flow and interstitial pH [J]. Scand J Gastroenterol, 1999; 34(4)∶439.
11. Toyama MT, Patel AG, Nguyen T, et al. Effect of ethanol on pancreatic interstitial pH and blood flow in cats with chronic pancreatitis [J]. Ann Surg, 1997; 225(2)∶223.
12. Patel AG, Reber PU, Toyama MT, et al. Effect of pancreaticojejunostomy on fibrosis, pancreatic blood flow, and interstitial pH in chronic pancreatitis: a feline model [J]. Ann Surg, 1999; 230(5)∶672.
13. Reber PU, Patel AG, Lewis MP, et al. Stenting does not decompress the pancreatic duct as effective as surgery in experimental chronic pancreatitis [J]. Surgery, 1998; 124(3)∶561.
14. 李春艷，崔美芝，成曉松，等. 急性胰腺炎動物模型概況 [J]. 哈爾濱醫(yī)科大學(xué)學(xué)報, 1998; 32(4)∶308.
15. 姜永鋒，張延齡. 急性胰腺炎的實(shí)驗(yàn)研究進(jìn)展 [J]. 國外醫(yī)學(xué)外科學(xué)分冊, 1996; 23(1)∶13.
16. NeuschwanderTetri BA, Burton FR, Presti ME, et al. Repetitive selflimited acute pancreatitis induces pancreatic fibrogenesis in the mouse [J]. Dig Dis Sci, 2000; 45(4)∶665.
17. Murayama KM, Barent BL, Gruber M, et al. Characterization of a novel model of pancreatic fibrosis and acinar atrophy [J]. J Gastrointest Surg, 1999; 3(4)∶418.
18. Miyahara T, Kawabuchi M, Goto M, et al. Morphological study of pancreatic endocrine in an experimental chronic pancreatitis with diabetes induced by stress and cerulein [J]. Ultrastruct Pathol, 1999; 23(3)∶171.
19. Merkord J, Weber H, Sparmann G, et al. The course of pancreatic fibrosis induced by dibutyltin dichloride (DBTC) [J]. Ann N Y Acad Sci, 1999; 880∶231.
20. Merkord J, Weber H, Jonas L, et al. The influence of ethanol on longterm effects of dibutyltin dichloride (DBTC) in pancreas and liver of rats [J]. Hum Exp Toxicol, 1998; 17(3)∶144.
21. Sparmann G, Merkord J, Jaschke A, et al. Pancreatic fibrosis in experimental pancreatitis induced by dibutyltin dichloride [J]. Gasteroenterology, 1997; 112(5)∶1664.
22. Sparmann G, Behrend S, Merkord J, et al. Cytokine mRNA levels and lymphocyte infiltration in pancreatic tissue during experimental chronic pancreatitis induced by dibutyltin dichloride [J]. Dig Dis Sci, 2001; 46(8)∶1647.
23. Freiburghaus AU, Redha F, Ammann RW. Does actue pancreatitis progress to chronic pancreatitis? A microvascular pancreatitis model in the rat [J]. Pancreas, 1995; 11(4)∶374.
24. Tanaka T, Ichiba Y, Miura Y, et al. Canine model of chronic pancreatitis due to chronic ischemia [J]. Digestion, 1994; 55(2)∶86.
25. Kataoka K, Sasaki T, Yorizumi H, et al. Pathophsiologic studies of experimental chronic pancreatitis in rats induced by injection of zeinoleic acidlinoleic acid solution into the pancreatic duct [J]. Pancreas, 1998; 16(3)∶289.
26. Haber PS, Keogh GW, Apte MV, et al. Activation of pancreatic stellate cells in human and experimental pancreatic fibrosis [J]. Am J Pathol, 1999; 155(4)∶ 1087.
27. 梁林，馮凱琳，呂維富. 胰腺分裂在慢性胰腺炎病因?qū)W中的作用及其發(fā)病機(jī)制 [J]. 實(shí)用放射學(xué)雜志, 2000; 16(9)∶522.
28. Vogelmann R, Ruf D, Wagner M, et al. Effects of fibrogenic mediators on the development of pancreatic fibrosis in a TGFbeta1 transgenic mouse model [J]. Am J Physiol Gastrointest Liver Physiol, 2001; 280(1)∶ G164.
  1. 1. Etemad B, Whitcomb DC. Chronic pancreatitis: diagnosis, classification, and new genetic developments [J]. Gastroenterology, 2001; 120(3)∶682.
  2. 2. 佟衛(wèi)剛，盧實(shí)春，胡驥，等. 慢性胰腺炎的外科治療及遠(yuǎn)期療效 [J]. 中國普外基礎(chǔ)與臨床雜志, 1999; 6(6)∶347.
  3. 3. Ohashi K, Kim JH, Hara H, et al. WBN/Kob rats. A new spontaneously occurring model of chronic pancreatitis [J]. Int J pancreatol, 1990; 6(4)∶231.
  4. 4. Su SB, Motoo Y, Xie MJ, et al. Expression of transforming growth factorbeta in spontaneous chronic pancreatitis in the WBN/Kob rat [J]. Dig Dis Sci, 2000; 45(1)∶151.
  5. 5. 耿智敏，向國安，韓慶，等. 轉(zhuǎn)化生長因子β1在膽管愈合過程中的表達(dá)及意義 [J]. 中國普外基礎(chǔ)與臨床雜志, 2000; 7(6)∶362.
  6. 6. Su SB, Motoo Y, Iovanna JL, et al. Effect of camostat mesilate on the expression of pancreatitisassociated protein (PAP), p8, and cytokines in rat spontaneous chronic pancreatitis [J]. Pancreas, 2001; 23(2)∶134.
  7. 7. Su SB, Motoo Y, Xie MJ, et al. Apoptosis in rat spontaneous chronic pancreatis: role of the Fas and Fas ligand system [J]. Dig Dis Sci, 2001; 46(1)∶166.
  8. 8. Widdison AL, Alvarez C, Schwarz M, et al. The influence of ethanol on pancreatic blood flow in cats with chronic pancreatitis [J]. Surgery, 1992; 112(2)∶202.
  9. 9. Karanjia ND, Widdison AL, Leung F, et al. Compartment syndrome in experimental chronic obstructive pancreatitis: effect of decompressing the main pancreatic duct [J]. Br J Surg, 1994; 81(2)∶ 259.
  10. 10. Reber PU, Patel AG, Toyama MT, et al. Feline model of chronic obstructive pancreatitis: effects of acute pancreatic duct decompression on blood flow and interstitial pH [J]. Scand J Gastroenterol, 1999; 34(4)∶439.
  11. 11. Toyama MT, Patel AG, Nguyen T, et al. Effect of ethanol on pancreatic interstitial pH and blood flow in cats with chronic pancreatitis [J]. Ann Surg, 1997; 225(2)∶223.
  12. 12. Patel AG, Reber PU, Toyama MT, et al. Effect of pancreaticojejunostomy on fibrosis, pancreatic blood flow, and interstitial pH in chronic pancreatitis: a feline model [J]. Ann Surg, 1999; 230(5)∶672.
  13. 13. Reber PU, Patel AG, Lewis MP, et al. Stenting does not decompress the pancreatic duct as effective as surgery in experimental chronic pancreatitis [J]. Surgery, 1998; 124(3)∶561.
  14. 14. 李春艷，崔美芝，成曉松，等. 急性胰腺炎動物模型概況 [J]. 哈爾濱醫(yī)科大學(xué)學(xué)報, 1998; 32(4)∶308.
  15. 15. 姜永鋒，張延齡. 急性胰腺炎的實(shí)驗(yàn)研究進(jìn)展 [J]. 國外醫(yī)學(xué)外科學(xué)分冊, 1996; 23(1)∶13.
  16. 16. NeuschwanderTetri BA, Burton FR, Presti ME, et al. Repetitive selflimited acute pancreatitis induces pancreatic fibrogenesis in the mouse [J]. Dig Dis Sci, 2000; 45(4)∶665.
  17. 17. Murayama KM, Barent BL, Gruber M, et al. Characterization of a novel model of pancreatic fibrosis and acinar atrophy [J]. J Gastrointest Surg, 1999; 3(4)∶418.
  18. 18. Miyahara T, Kawabuchi M, Goto M, et al. Morphological study of pancreatic endocrine in an experimental chronic pancreatitis with diabetes induced by stress and cerulein [J]. Ultrastruct Pathol, 1999; 23(3)∶171.
  19. 19. Merkord J, Weber H, Sparmann G, et al. The course of pancreatic fibrosis induced by dibutyltin dichloride (DBTC) [J]. Ann N Y Acad Sci, 1999; 880∶231.
  20. 20. Merkord J, Weber H, Jonas L, et al. The influence of ethanol on longterm effects of dibutyltin dichloride (DBTC) in pancreas and liver of rats [J]. Hum Exp Toxicol, 1998; 17(3)∶144.
  21. 21. Sparmann G, Merkord J, Jaschke A, et al. Pancreatic fibrosis in experimental pancreatitis induced by dibutyltin dichloride [J]. Gasteroenterology, 1997; 112(5)∶1664.
  22. 22. Sparmann G, Behrend S, Merkord J, et al. Cytokine mRNA levels and lymphocyte infiltration in pancreatic tissue during experimental chronic pancreatitis induced by dibutyltin dichloride [J]. Dig Dis Sci, 2001; 46(8)∶1647.
  23. 23. Freiburghaus AU, Redha F, Ammann RW. Does actue pancreatitis progress to chronic pancreatitis? A microvascular pancreatitis model in the rat [J]. Pancreas, 1995; 11(4)∶374.
  24. 24. Tanaka T, Ichiba Y, Miura Y, et al. Canine model of chronic pancreatitis due to chronic ischemia [J]. Digestion, 1994; 55(2)∶86.
  25. 25. Kataoka K, Sasaki T, Yorizumi H, et al. Pathophsiologic studies of experimental chronic pancreatitis in rats induced by injection of zeinoleic acidlinoleic acid solution into the pancreatic duct [J]. Pancreas, 1998; 16(3)∶289.
  26. 26. Haber PS, Keogh GW, Apte MV, et al. Activation of pancreatic stellate cells in human and experimental pancreatic fibrosis [J]. Am J Pathol, 1999; 155(4)∶ 1087.
  27. 27. 梁林，馮凱琳，呂維富. 胰腺分裂在慢性胰腺炎病因?qū)W中的作用及其發(fā)病機(jī)制 [J]. 實(shí)用放射學(xué)雜志, 2000; 16(9)∶522.
  28. 28. Vogelmann R, Ruf D, Wagner M, et al. Effects of fibrogenic mediators on the development of pancreatic fibrosis in a TGFbeta1 transgenic mouse model [J]. Am J Physiol Gastrointest Liver Physiol, 2001; 280(1)∶ G164.