目的探討促凋亡基因bad在原發(fā)性肝內(nèi)膽管細(xì)胞癌（ICC）中的表達(dá)及其與腫瘤分化程度的關(guān)系。方法采用免疫組織化學(xué)Dako Envision方法，對(duì)48例原發(fā)性肝內(nèi)膽管細(xì)胞癌（ICC組）和25例肝細(xì)胞肝癌的癌旁肝組織匯管區(qū)膽管上皮組織（對(duì)照組）中bad基因的表達(dá)情況進(jìn)行檢測(cè)。結(jié)果bad基因在ICC中的表達(dá)陽(yáng)性率明顯高于對(duì)照組（P＜0.01）； 在中、低分化的ICC中bad基因的陽(yáng)性表達(dá)水平明顯高于高分化的ICC（P lt;0.05）。結(jié)論在低分化的膽管細(xì)胞癌中bad基因的表達(dá)明顯增強(qiáng),bad基因的表達(dá)與腫瘤的分化程度相關(guān)。
引用本文： 閆慶國(guó),師建國(guó),王文勇,李玉松,黃高昇,王文亮. 促凋亡基因bad的表達(dá)與肝內(nèi)膽管細(xì)胞癌分化的關(guān)系. 中國(guó)普外基礎(chǔ)與臨床雜志, 2003, 10(3): 253-255. doi: 復(fù)制
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- 1. Van Cruchten S, Van Den Broeck W. Morphological and biochemical aspects of apoptosis, oncosis and necrosis [J]. Anat Histol Embryol, 2002; 31(4)∶214.
- 2. 閆慶國(guó)，王文亮，黃高昇. 促細(xì)胞凋亡bax在膽管癌中的表達(dá)與意義 [J]. 第四軍醫(yī)大學(xué)學(xué)報(bào)，1997； 18（2）∶139.
- 3. 閆慶國(guó)，王文亮，黃高昇. 膽管癌中抗凋亡基因bcl2的表達(dá)及意義 [J]. 臨床肝膽病雜志，1997； 13（4）∶194.
- 4. Ottilie S, Diaz JL, Horne W, et al. Dimerization properties of human BAD. Identification of a BH3 domain and analysis of its binding to mutant bcl2 and bclxL proteins [J]. J Biol Chem,1997; 272(49)∶30866.
- 5. Kelekar A, Chang BS, Harlan JE, et al. Bad is a BH3 domaincontaining protein that forms an inactivating dimer with bclxL [J]. Mol Cell Biol, 1997; 17(12)∶7040.
- 6. Hong JR, Wu JL.Induction of apoptotic death in cells via bad gene expression by infectious pancreatic necrosis virus infection [J]. Cell Death Differ, 2002; 9(2)∶113.
- 7. Ichinose M, Liu XH, Hagihara N, et al.Extracellular bad fused to toxin transport domains induces apoptosis [J]. Cancer Res, 2002; 62(5)∶1433.
- 8. Kohler T, Schill C, Deininger MW, et al.High bad and bax mRNA expression correlate with negative outcome in acute myeloid leukemia (AML) [J]. Leukemia, 2002; 16(1)∶22.
- 9. Schimmer AD, Hedley DW, Pham NA, et al. BAD induces apoptosis in cells overexpressing Bcl2 or BclxL without loss of mitochondrial membrane potential [J]. Leuk Lymphoma,2001; 42(3)∶429.
- 10. Hattori T, Ookawa N, Fujita R, et al. Heterodimerization of bcl2 and bclx(L) with bax and bad in colorectal cancer [J]. Acta Oncol, 2000; 39(4)∶495.