• 1.四川大學(xué)華西醫(yī)院普外科（成都610041）;;
  • 2.廣西醫(yī)科大學(xué)附一院普外科（南寧530021）;;
  • 3.日本產(chǎn)業(yè)醫(yī)科大學(xué)生態(tài)科學(xué)研究所腫瘤部（Kitakyushu 807-8555，Japan）;;
  • 4.廣西醫(yī)科大學(xué)附一院檢驗(yàn)科;;
  • 5.廣西扶綏縣腫瘤防治研究所（扶綏532100）;

的探討一套高效液相色譜電化學(xué)系統(tǒng)檢測(cè)尿8羥基脫氧鳥(niǎo)苷(8OHdG)水平的方法學(xué)。方法采用ESA 5600 Coularray系統(tǒng)檢測(cè)廣西扶綏縣肝癌戶人群21例及南寧市對(duì)照人群63例尿8OHdG水平。結(jié)果8OHdG峰與雜質(zhì)峰分離良好； 8OHdG濃度在0.01～15 ng/ml間呈良好線性； 保留時(shí)間、標(biāo)樣和內(nèi)對(duì)照的重復(fù)性試驗(yàn)的批內(nèi)差異(CV)均小于8%； 標(biāo)樣(F=0.002, P=1.000)和內(nèi)對(duì)照(F=2.965, P=0.082)的重復(fù)性試驗(yàn)的批間差異經(jīng)方差分析均無(wú)顯著性意義。采用上述方法測(cè)得84例總的尿8OHdG水平為（5.26±0.41）ng/mg肌酐，高于同類(lèi)研究的檢測(cè)值〔（4.62±0.091）ng/mg肌酐, n=32〕，但兩地人群間尿8OHdG水平差異無(wú)顯著性意義。結(jié)論這是對(duì)肝癌高發(fā)區(qū)高危人群氧化性DNA損傷的首次報(bào)告。通過(guò)謹(jǐn)慎設(shè)計(jì)試驗(yàn)和優(yōu)化試驗(yàn)參數(shù)，該系統(tǒng)對(duì)8OHdG峰的分離優(yōu)于以往報(bào)告，適用于尿8OHdG的高通量定量分析，可應(yīng)用于人群氧化性ＤＮＡ損傷的研究。

引用本文： 彭濤,嚴(yán)律南,劉志明,葛西宏,彭民浩,黎樂(lè)群. 高效液相色譜電化學(xué)法檢測(cè)尿液8羥基脫氧鳥(niǎo)苷水平. 中國(guó)普外基礎(chǔ)與臨床雜志, 2003, 10(3): 281-283. doi: 復(fù)制

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  1. 1. Halliwell B. Free radical, antioxidants, and human disease: curiosity, cause, or consequence? [J]. Lancet, 1994； 344(13)∶721.
  2. 2. Grollman AP, Moriya M. Mutagenesis by 8oxoguanine: an enemy within [J]. Trends Genet, 1993； 9(7)∶246.
  3. 3. Kasai H. Analysis of a form of oxidative DNA damage, 8hydroxy2’deoxyguanosine, as a marker of cellular oxidative stress during carcinogenesis [J]. Mutat Res, 1997； 387(3)∶147.
  4. 4. Kasai H, IwamotoTanaka N, Miyamoto T, et al. Life style and urinary 8hydroxydeoxyguanosine, a marker of oxidative dna damage: effects of exercise, working conditions, meat intake, body mass index, and smoking [J]. Jpn J Cancer Res, 2001； 92(1)∶9.
  5. 5. Bogdanov MB, Beal MF, McCabe DR, et al. A carbon columnbased liquid chromatography electrochemical approach to routine 8hydroxy2’deoxyguanosine measurements in urine and other biologic matrices: a oneyear evaluation of methods [J]. Free Radic Biol Med, 1999； 27(5-6)∶647.
  6. 6. Tagesson C, Chabiuk D, Axelson O,et al. Increased urinary excretion of the oxidative DNA adduct, 8hydroxydeoxyguanosine, as a possible early indicator of occupational cancer hazards in the asbestos, rubber, and azodye industies [J]. Pol J Occup Med Environ Health, 1993； 6(4)∶357.
  7. 7. Loft S, Vistisen K, Ewertz M, et al. Oxidative DNA damage estimated by 8hydroxydeoxyguanosine excretion in humans: influence of smoking, gender and body mass index [J]. Carcinogenesis, 1992； 13(12)∶2241.
  8. 8. Acworth IN, McCabe DR, Maher TJ. The analysis of free radical, their reaction products, and antioxidants. In: Baskin SI, Salem H. Eds. Oxidants, antioxidants, and free radicals [M]. Washington: Taylor & Francis， 1997∶23-77.