目的探討甘氨酸對大鼠肝臟熱缺血再灌注后肝竇內(nèi)皮細胞損傷的保護作用及其機理。方法取健康雄性SD大鼠72只，制備成鼠肝熱缺血再灌注模型,而后隨機分成正常對照組、缺血再灌注組、士的寧＋甘氨酸治療組和甘氨酸治療組, 每組各18只。分別于再灌注后1、3、24 h檢測血漿中內(nèi)皮素（ET）、透明質(zhì)酸（HA）、腫瘤壞死因子α （TNFα）、谷丙轉(zhuǎn)氨酶（ALT）及肝組織中超氧化物歧化酶（SOD）含量的變化，并在光鏡下觀察肝竇內(nèi)皮細胞的病理改變。結(jié)果在再灌注后的不同時點，甘氨酸治療組中ET、HA、TNFα及ALT含量較缺血再灌注組顯著降低(P＜0.01或P＜0.05），SOD值相應升高，同時光鏡下肝竇內(nèi)皮細胞的病理變化明顯改善，士的寧可部分拮抗甘氨酸的作用。結(jié)論甘氨酸對鼠肝熱缺血再灌注后肝竇內(nèi)皮細胞的損傷具有保護作用。推測這種作用可能與肝竇內(nèi)皮細胞及枯否細胞膜上的甘氨酸受體密切相關。
引用本文： 舒明,熊本京,傅華群,蔣筱強,鄒書兵. 甘氨酸對鼠肝熱缺血再灌注后肝竇內(nèi)皮細胞損傷的保護作用. 中國普外基礎與臨床雜志, 2003, 10(4): 351-354. doi: 復制
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