目的 探討缺血預處理（ischemic preconditioning,IP）對大鼠移植肝臟缺血再灌注損傷的保護作用。方法采用SD大鼠原位肝移植動物模型，供肝冷保存時間100 min，無肝期25 min。64只SD大鼠隨機均分成兩組： 對照組，獲取供肝前僅以肝素生理鹽水經(jīng)門靜脈灌注； IP組，獲取供肝前阻斷肝門血供10 min，再灌注10 min，然后再以肝素生理鹽水經(jīng)門靜脈灌注。每組受體的一半（n=8）用于觀察存活率，另一半(n=8)用于移植肝臟再灌注2 h后取血及肝臟檢測。結果IP組的1 w存活率、膽汁分泌量、抗氧化酶活力、血清NO水平均明顯高于對照組(P＜0.05)，血清ALT、AST、LDH、TNF及肝組織中的過氧化產(chǎn)物含量均明顯低于對照組(P＜0.05)，組織的病理改變也輕于對照組。結論IP能夠提高血清NO水平，降低血清TNF含量，對大鼠移植肝臟的缺血再灌注損傷具有保護作用。
引用本文： 涂兵,嚴律南,劉智敏. 缺血預處理對大鼠移植肝臟缺血再灌注損傷的保護作用. 中國普外基礎與臨床雜志, 2002, 9(2): 89-92. doi: 復制
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- 4. Ploeg RJ,D’Alessandro AM,Knechtle SJ,et al.Risk factors for primary dysfunction after liver transplantation:a multivariate analysis \[J\].Transplantation,1993; 55(4)∶807.
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- 6. Murry CE,Jennings RB,Reimer KA.Preconditioning with ischemia: a delay of lethal cell injury in ischemic myocardium \[J\].Circulation,1986; 74(5)∶1124.
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- 9. Nishida T,Gatmaitan Z,Che MX,et al.Rat liver canalicular membrane vesicles contain an ATPdependent bile acid transport system \[J\].Proc Natl Acad Sci USA,1991; 88(15)∶6590.
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