• 1.浙江省衢州市人民醫(yī)院（衢州324000）;;
  • 2.浙江大學(xué)醫(yī)學(xué)院附屬一院（杭州310003）;

目的研究兔股靜脈延遲移植血漿內(nèi)皮素(ET)和一氧化氮(NO)含量的變化及其與血管痙攣、靜脈移植的關(guān)系。方法用放射免疫方法測(cè)定局部血漿ET的含量，用硝酸還原酶法及紫外可見(jiàn)分光光度計(jì)測(cè)定局部血漿中NO-2/NO-3含量，間接反映NO水平。結(jié)果游離股靜脈(FV)局部血漿ET含量術(shù)后2 h和4 h明顯增高(P＜0.01)，術(shù)后8 h也增高(P＜0.05)； 局部血漿NO含量術(shù)后2 h和4 h下降非常顯著(P＜0.01)，術(shù)后24 h則又顯著增高(P＜0.05)。結(jié)論游離(未切斷)FV術(shù)后16～24 h，延遲切斷準(zhǔn)備移植的FV血漿中ET含量恢復(fù)正常，NO含量明顯增高，是移植靜脈血管危象發(fā)生率下降的原因，此時(shí)為切斷靜脈進(jìn)行移植的最佳時(shí)間。

引用本文： 王李華,鄭樹(shù)森,余耀生,潘松齡,申桂娟,張鴻坤. 兔股靜脈延遲移植內(nèi)皮素和一氧化氮變化的意義. 中國(guó)普外基礎(chǔ)與臨床雜志, 2002, 9(4): 235-237. doi: 復(fù)制

1. Hirata Y, Itoh K, Ando K, et al. Plasma endothelin levels during surgery [J]. N Engl J Med, 1989; 321(24)∶1686.
2. 段志泉, 張強(qiáng)著. 實(shí)用血管外科 [M]. 第 1版. 沈陽(yáng)： 遼寧科學(xué)技術(shù)出版社，1999∶1～55.
3. Stoyanovsky D, Murphy T, Anno PR, et al. Nitric oxide activates skeletal and cardiac ryanodine receptors [J]. Cell Calcium, 1997; 21(1)∶19.
4. Wagner OF, Christ G, Wojta J, et al. Polar secretion of endothelin1 by cultured endothelial cells [J]. J Biol Chem, 1992; 267(23)∶16066.
5. Kourembanas S, Marsden PA, McQuillan LP, et al. Hypoxia induces endothelin gene expression and secretion in culture human endothelium [J]. J Clin Invest, 1991; 88(3)∶1054.
6. Morganti A, Giussani M, Ghio F, et al. Endothelinreleasing stimuli and calcium antagonists in normal and pathological conditions [J]. J Hypertention Suppl, 1994; 12 (1)∶S27.
7. Poderoso JJ, Peralta JG, Lisdero CL, et al. Nitric oxide regulates oxygen uptake and hydrogen peroxide release by the isolated beating rat heart [J]. Am J Physiol, 1998; 274(1 Pt 1)∶C112.
8. Li Z, Chapleau MW, Bates JN, et al. Nitric oxide as an autocrine regulator of sodium currents in baroreceptor neurons [J]. Neuron, 1998; 20(5)∶1039.
  1. 1. Hirata Y, Itoh K, Ando K, et al. Plasma endothelin levels during surgery [J]. N Engl J Med, 1989; 321(24)∶1686.
  2. 2. 段志泉, 張強(qiáng)著. 實(shí)用血管外科 [M]. 第 1版. 沈陽(yáng)： 遼寧科學(xué)技術(shù)出版社，1999∶1～55.
  3. 3. Stoyanovsky D, Murphy T, Anno PR, et al. Nitric oxide activates skeletal and cardiac ryanodine receptors [J]. Cell Calcium, 1997; 21(1)∶19.
  4. 4. Wagner OF, Christ G, Wojta J, et al. Polar secretion of endothelin1 by cultured endothelial cells [J]. J Biol Chem, 1992; 267(23)∶16066.
  5. 5. Kourembanas S, Marsden PA, McQuillan LP, et al. Hypoxia induces endothelin gene expression and secretion in culture human endothelium [J]. J Clin Invest, 1991; 88(3)∶1054.
  6. 6. Morganti A, Giussani M, Ghio F, et al. Endothelinreleasing stimuli and calcium antagonists in normal and pathological conditions [J]. J Hypertention Suppl, 1994; 12 (1)∶S27.
  7. 7. Poderoso JJ, Peralta JG, Lisdero CL, et al. Nitric oxide regulates oxygen uptake and hydrogen peroxide release by the isolated beating rat heart [J]. Am J Physiol, 1998; 274(1 Pt 1)∶C112.
  8. 8. Li Z, Chapleau MW, Bates JN, et al. Nitric oxide as an autocrine regulator of sodium currents in baroreceptor neurons [J]. Neuron, 1998; 20(5)∶1039.