目的探討自主神經(jīng)控制對人肝細胞增殖的影響及其機理，并對增殖相關(guān)受體定位。方法用去甲腎上腺素（NE）及其拮抗劑及激動劑和乙酰膽堿（Ach）及其阻滯劑作用于人傳代正常肝L02細胞及肝癌Bel7402細胞，分別于4 h、24 h、48 h及72 h用改良MTT法檢測上述藥物對細胞增殖的影響； 免疫組織化學染色法對肝L02細胞的腎上腺素受體（α1BAR及β2AR）和表皮生長因子受體（EGFR）定位。結(jié)果NE對人正常肝細胞及肝癌細胞具有促增殖作用，且隨濃度增加其促增殖作用也增加，4 h的增殖率較其它時間點顯著增高（P＜0.05）； NE+心得安組的作用與NE組間差異無顯著性意義； 二羥苯基異丙氨基乙醇的作用不明顯; Ach對人正常肝細胞的增殖具有抑制作用，且隨濃度增加其抑制作用也增加，加用阿托品在24 h及48 h與Ach比抑制率顯著降低（P＜0.05）； 琥珀膽堿組在24 h、48 h及72 h對細胞的增殖也具有抑制作用（P＜0.05，P＜0.01）。所有人肝L02細胞都呈α1BAR、β2AR及EGFR免疫反應陽性，主要定位于胞膜及胞漿。結(jié)論血清存在下，交感神經(jīng)遞質(zhì)NE早期對人正常肝細胞L02及人肝癌細胞Bel7402具有促增殖作用，其作用是通過激動αAR實現(xiàn)的, βAR對人肝細胞增殖無影響。副交感神經(jīng)遞質(zhì)Ach對人正常肝細胞L02的增殖具有抑制作用，其作用是通過激動M及N型膽堿受體實現(xiàn)的。
引用本文： 李立,陳剛,郭永章. 神經(jīng)遞質(zhì)對肝細胞增殖的影響△. 中國普外基礎與臨床雜志, 2002, 9(4): 238-241. doi: 復制
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