目的探討自主神經(jīng)控制對人肝細胞增殖的影響及其機理，并對增殖相關(guān)受體定位。方法用去甲腎上腺素（NE）及其拮抗劑及激動劑和乙酰膽堿（Ach）及其阻滯劑作用于人傳代正常肝L02細胞及肝癌Bel7402細胞，分別于4 h、24 h、48 h及72 h用改良MTT法檢測上述藥物對細胞增殖的影響； 免疫組織化學(xué)染色法對肝L02細胞的腎上腺素受體（α1BAR及β2AR）和表皮生長因子受體（EGFR）定位。結(jié)果NE對人正常肝細胞及肝癌細胞具有促增殖作用，且隨濃度增加其促增殖作用也增加，4 h的增殖率較其它時間點顯著增高（P＜0.05）； NE+心得安組的作用與NE組間差異無顯著性意義； 二羥苯基異丙氨基乙醇的作用不明顯; Ach對人正常肝細胞的增殖具有抑制作用，且隨濃度增加其抑制作用也增加，加用阿托品在24 h及48 h與Ach比抑制率顯著降低（P＜0.05）； 琥珀膽堿組在24 h、48 h及72 h對細胞的增殖也具有抑制作用（P＜0.05，P＜0.01）。所有人肝L02細胞都呈α1BAR、β2AR及EGFR免疫反應(yīng)陽性，主要定位于胞膜及胞漿。結(jié)論血清存在下，交感神經(jīng)遞質(zhì)NE早期對人正常肝細胞L02及人肝癌細胞Bel7402具有促增殖作用，其作用是通過激動αAR實現(xiàn)的, βAR對人肝細胞增殖無影響。副交感神經(jīng)遞質(zhì)Ach對人正常肝細胞L02的增殖具有抑制作用，其作用是通過激動M及N型膽堿受體實現(xiàn)的。
引用本文： 李立,陳剛,郭永章. 神經(jīng)遞質(zhì)對肝細胞增殖的影響△. 中國普外基礎(chǔ)與臨床雜志, 2002, 9(4): 238-241. doi: 復(fù)制
版權(quán)信息： ?四川大學(xué)華西醫(yī)院華西期刊社《中國普外基礎(chǔ)與臨床雜志》版權(quán)所有，未經(jīng)授權(quán)不得轉(zhuǎn)載、改編
| 1. | Cruise JL, Knechtle SJ, Bollinger RR, et al. Alpha 1adrenergic effects and liver regeneration [Ｊ]. Hepatology，1987； 7(6)∶1189. |
| 2. | Refsnes M, Thoresen GH,Sandnes D,et al. Stimulatory and inhibitory effects of catecholamines on DNA synthesis in primary rat hepatocyte cultures: role of alpha 1and betaadrenergic mechanisms [Ｊ]. J Cell Physiol,1992; 151(1)∶164. |
| 3. | Kajiyama Y, Ui M. Differential mitogenic actions of alpha 1 and betaadrenergic agonists on rat hepatocyte [Ｊ]. Cell Signal,1998; 10(4)∶241. |
| 4. | Kimura M, Ogihara M. Proliferation of adult rat hepatocytes by hepatocyte growth factor is potentiated by both phenylephrine and metaproterenol [Ｊ]. J Pharmacol Exp Ther,1997; 282(3)∶ 1146. |
| 5. | Elaeva NL, Stroikov IuN. Mechanism of action of eserine on DNA synthesis in rat liver [Ｊ]. Biull Eksp Biol Med, 1979; 87(1)∶24. |
| 6. | Kiba T, Saito S, Numata K,et al. Expresson of apoptosis on rat liver by hepatic vagus hyperactivity after ventromedial hypothalamic lesioning [Ｊ]. Am J Physiol Gastrointest Liver Physiol，2001; 280(5)∶G958. |
| 7. | 呂寶璋, 田英. 受體學(xué)概論 [M]. 北京: 科學(xué)出版社, 1991∶224~232. |
| 8. | 黃曉峰, 張遠強, 樸永安,等. 表皮生長因子及其受體在大鼠肝癌細胞的免疫細胞化學(xué)定位 [Ｊ]. 科學(xué)通報,1996; 41(16)∶1513. |
- 1. Cruise JL, Knechtle SJ, Bollinger RR, et al. Alpha 1adrenergic effects and liver regeneration [Ｊ]. Hepatology，1987； 7(6)∶1189.
- 2. Refsnes M, Thoresen GH,Sandnes D,et al. Stimulatory and inhibitory effects of catecholamines on DNA synthesis in primary rat hepatocyte cultures: role of alpha 1and betaadrenergic mechanisms [Ｊ]. J Cell Physiol,1992; 151(1)∶164.
- 3. Kajiyama Y, Ui M. Differential mitogenic actions of alpha 1 and betaadrenergic agonists on rat hepatocyte [Ｊ]. Cell Signal,1998; 10(4)∶241.
- 4. Kimura M, Ogihara M. Proliferation of adult rat hepatocytes by hepatocyte growth factor is potentiated by both phenylephrine and metaproterenol [Ｊ]. J Pharmacol Exp Ther,1997; 282(3)∶ 1146.
- 5. Elaeva NL, Stroikov IuN. Mechanism of action of eserine on DNA synthesis in rat liver [Ｊ]. Biull Eksp Biol Med, 1979; 87(1)∶24.
- 6. Kiba T, Saito S, Numata K,et al. Expresson of apoptosis on rat liver by hepatic vagus hyperactivity after ventromedial hypothalamic lesioning [Ｊ]. Am J Physiol Gastrointest Liver Physiol，2001; 280(5)∶G958.
- 7. 呂寶璋, 田英. 受體學(xué)概論 [M]. 北京: 科學(xué)出版社, 1991∶224~232.
- 8. 黃曉峰, 張遠強, 樸永安,等. 表皮生長因子及其受體在大鼠肝癌細胞的免疫細胞化學(xué)定位 [Ｊ]. 科學(xué)通報,1996; 41(16)∶1513.