• 四川大學(xué)華西醫(yī)院普外科（成都610041）;;
  • △研究生，現(xiàn)在四川省人民醫(yī)院普外科（成都610072）;

目的探討Ⅳ型膠原蛋白在乳腺癌中的表達(dá)情況及其與臨床病理間的關(guān)系。方法應(yīng)用Ⅳ型膠原蛋白單克隆抗體，采用鏈親和素（LSAB）法檢測(cè)93例乳腺惡性腫瘤及5例良性腫瘤組織中Ⅳ型膠原蛋白的表達(dá)。結(jié)果Ⅳ型膠原蛋白表達(dá)與乳腺癌患者腋窩淋巴結(jié)受累、生存時(shí)間及腫瘤大小有關(guān)。Ⅳ型膠原蛋白表達(dá)缺乏組5年生存率為29.69%，明顯低于表達(dá)完整組的5年生存率（81.43%），P＜0.05； 隨著腫瘤的長(zhǎng)大，Ⅳ型膠原表達(dá)完整率由53.8%降至10.3%，兩者間呈正相關(guān)（r=0.219）。結(jié)論檢測(cè)Ⅳ型膠原在乳腺癌基底膜中的表達(dá)狀況，將有助于乳腺癌預(yù)后的判斷及治療方案的制訂。

引用本文： 王曉東,楊金巧. Ⅳ型膠原在乳腺癌中的表達(dá)及其意義. 中國(guó)普外基礎(chǔ)與臨床雜志, 2002, 9(5): 303-305. doi: 復(fù)制

1. Fridman R, Alon Y, Doljanski F, et al. Cell interaction with the extracellular matrices produced by endothelial and fibroblasts [J]. Exp Cell Res, 1985; 158(2)∶461.
2. Aznavoorian S, Murphy AM, StellerStevenson WG, et al. Molecular aspects of tumor cell invasion and metastasis [J]. Cancer, 1993; 71(4)∶1368.
3. Sobin LH, Wittekind C. TNM classification of malignant tumors [M]. 5th ed. New York: Gohn Wiley and Sons Inc, 1997∶123-167.
4. Campo E, Merino MJ, Tavassoli FA, et al. Evaluation of basement membrane components and the 72 kDa type Ⅳ Collagenase in serous tumors of the ovary [J]. Am J Surg Pathol, 1992; 16(5)∶500.
5. Birembaut P, Caron Y, Adnet JJ, et al. Usefulness of basement membrane markers in tumoural pathology [J]. J Pathol, 1985; 145(4)∶283.
6. Mahfouz SM, Chevallier M, Grimaud JA. Distribution of the major connective matrix components of the stromal reaction in breast carcinoma. An immunohistochemical study [J]. Cell Mol Biol, 1987; 33(4)∶453.
7. Albrechtsen R, Nielsen M, Wewer U, et al. Basement membrane changes in breast cancer detected by immunohistochemical staining for laminin [J]. Cancer Res, 1981; 41(12 Pt 1)∶5076.
8. Barsky SH, Rao CN, Grotendorst GR, et al. Increased content of type Ⅴ collagen in desmoplasia of human breast carcinoma [J]. Am J Path, 1981; 108（3）∶279.
9. Clavel C, Doco M, Lallemand A, et al. Detection by in situ hybridization of messenger RNAs of collagen type I and Ⅳ in murine mammary [J]. Int J Cancer, 1989; 44(3)∶548.
10. Liotta LA. Tumor invasion and metastasis: role of the basement membrane. WarnerLambert ParkeDavis Award lecture [J]. Am J Pathol, 1984; 117(3)∶339.
11. Liotta LA, StetlarStevenson W. Metalloproteinase and malignant conversion: does correlation imply causality? [J]. J Natl Cancer Inst, 1989; 81(8)∶556.
12. 劉剛，韓德昌，嚴(yán)全梅. 口腔鱗癌Ⅳ型膠原和層粘連蛋白的表達(dá)與頸淋巴結(jié)轉(zhuǎn)移的關(guān)系 [J]. 臨床口腔醫(yī)學(xué)雜志，2000； 16（2）∶74.
13. 張健康，李曉麗，李亮成，等. Ⅳ型膠原、層粘連蛋白、纖維粘連蛋白在胃癌中表達(dá)的意義 [J]. 山西醫(yī)科大學(xué)學(xué)報(bào)，2000； 31（2）∶114.
14. 寶建中，鄭建明，周霖，等. 癌基因蛋白p21、p185、EGFR及LN、Ⅳ型膠原在骨肉瘤中的表達(dá) [J]. 腫瘤防治研究，1999； 26（4）∶265.
15. Reed JA, Loganzo F Jr, Shea CR, et al. Loss of expression of the p16/cyclindependent kinase inhibitor 2 tumor suppressor gene in melanocytic lesions correlates with invasive stage of tumor progression [J]. Cancer Res, 1995; 55(13)∶2713.
  1. 1. Fridman R, Alon Y, Doljanski F, et al. Cell interaction with the extracellular matrices produced by endothelial and fibroblasts [J]. Exp Cell Res, 1985; 158(2)∶461.
  2. 2. Aznavoorian S, Murphy AM, StellerStevenson WG, et al. Molecular aspects of tumor cell invasion and metastasis [J]. Cancer, 1993; 71(4)∶1368.
  3. 3. Sobin LH, Wittekind C. TNM classification of malignant tumors [M]. 5th ed. New York: Gohn Wiley and Sons Inc, 1997∶123-167.
  4. 4. Campo E, Merino MJ, Tavassoli FA, et al. Evaluation of basement membrane components and the 72 kDa type Ⅳ Collagenase in serous tumors of the ovary [J]. Am J Surg Pathol, 1992; 16(5)∶500.
  5. 5. Birembaut P, Caron Y, Adnet JJ, et al. Usefulness of basement membrane markers in tumoural pathology [J]. J Pathol, 1985; 145(4)∶283.
  6. 6. Mahfouz SM, Chevallier M, Grimaud JA. Distribution of the major connective matrix components of the stromal reaction in breast carcinoma. An immunohistochemical study [J]. Cell Mol Biol, 1987; 33(4)∶453.
  7. 7. Albrechtsen R, Nielsen M, Wewer U, et al. Basement membrane changes in breast cancer detected by immunohistochemical staining for laminin [J]. Cancer Res, 1981; 41(12 Pt 1)∶5076.
  8. 8. Barsky SH, Rao CN, Grotendorst GR, et al. Increased content of type Ⅴ collagen in desmoplasia of human breast carcinoma [J]. Am J Path, 1981; 108（3）∶279.
  9. 9. Clavel C, Doco M, Lallemand A, et al. Detection by in situ hybridization of messenger RNAs of collagen type I and Ⅳ in murine mammary [J]. Int J Cancer, 1989; 44(3)∶548.
  10. 10. Liotta LA. Tumor invasion and metastasis: role of the basement membrane. WarnerLambert ParkeDavis Award lecture [J]. Am J Pathol, 1984; 117(3)∶339.
  11. 11. Liotta LA, StetlarStevenson W. Metalloproteinase and malignant conversion: does correlation imply causality? [J]. J Natl Cancer Inst, 1989; 81(8)∶556.
  12. 12. 劉剛，韓德昌，嚴(yán)全梅. 口腔鱗癌Ⅳ型膠原和層粘連蛋白的表達(dá)與頸淋巴結(jié)轉(zhuǎn)移的關(guān)系 [J]. 臨床口腔醫(yī)學(xué)雜志，2000； 16（2）∶74.
  13. 13. 張健康，李曉麗，李亮成，等. Ⅳ型膠原、層粘連蛋白、纖維粘連蛋白在胃癌中表達(dá)的意義 [J]. 山西醫(yī)科大學(xué)學(xué)報(bào)，2000； 31（2）∶114.
  14. 14. 寶建中，鄭建明，周霖，等. 癌基因蛋白p21、p185、EGFR及LN、Ⅳ型膠原在骨肉瘤中的表達(dá) [J]. 腫瘤防治研究，1999； 26（4）∶265.
  15. 15. Reed JA, Loganzo F Jr, Shea CR, et al. Loss of expression of the p16/cyclindependent kinase inhibitor 2 tumor suppressor gene in melanocytic lesions correlates with invasive stage of tumor progression [J]. Cancer Res, 1995; 55(13)∶2713.