目的研究結(jié)、直腸癌血行轉(zhuǎn)移與腫瘤轉(zhuǎn)移相關(guān)因子間的關(guān)系。方法采用逆轉(zhuǎn)錄聚合酶鏈反應(yīng)（RTPCR）技術(shù),檢測(cè)了50例結(jié)、直腸癌患者血液中CK20 mRNA的表達(dá)，并用免疫組化方法檢測(cè)了癌組織中CD44v6和p53的表達(dá)。結(jié)果CK20 mRNA陽(yáng)性表達(dá)與癌組織中p53和CD44v6的表達(dá)之間存在著顯著相關(guān)性。結(jié)論手術(shù)前監(jiān)測(cè)血液中CK20 mRNA的表達(dá)并結(jié)合癌組織中p53和CD44v6表達(dá)的檢測(cè)，對(duì)于預(yù)測(cè)結(jié)、直腸癌血行轉(zhuǎn)移和指導(dǎo)術(shù)后治療具有重要參考價(jià)值。
引用本文： 孫念緒,王德虎,唐波. 結(jié)、直腸癌血行轉(zhuǎn)移與腫瘤轉(zhuǎn)移因子的關(guān)系. 中國(guó)普外基礎(chǔ)與臨床雜志, 2002, 9(5): 348-351. doi: 復(fù)制
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- 1. 高進(jìn)主編. 癌的侵襲與轉(zhuǎn)移 [M].第1版. 北京： 北京醫(yī)科大學(xué)中國(guó)協(xié)和醫(yī)科大學(xué)聯(lián)合出版社，1996∶200～206.
- 2. 郭君其綜述. 大腸癌微轉(zhuǎn)移的研究現(xiàn)狀及進(jìn)展 [J].國(guó)外醫(yī)學(xué)腫瘤學(xué)分冊(cè)，1997； 24(5)∶281.
- 3. Garcia RL,Coltrera MD,Gown AM.Analysis of proliferative grade using antiPCNA/cyclin monoclonal antibodies in fixed,embedded tissues. Comparison with flow cytometric analysis [J]. Am J Pathol， 1989; 134(4)∶733.
- 4. 駱成玉，李世擁. 大腸癌患者門靜脈血癌細(xì)胞的檢測(cè)及臨床意義 [J].中華外科雜志，1999； 37（4）∶214.
- 5. Hillaire S,Barbu V,Boucher E, et al. Albumin messenger RNA as a marker of circulating hepatocytes in hepatocellular carcinoma [J]. Gastroenterology, 1994; 106(1)∶239.
- 6. Moll R,Lowe A,Laufer J, et al. Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies [J]. Am J Pathol, 1992; 140(2)∶427.
- 7. Datta YH, Adams PT,Drobyski WR, et al. Sensitive detection of occult breast cancer by the reversetranscriptase polymerase chain reaction [J]. J Clin Oncol, 1994; 12(3)∶475.
- 8. Dammrich J, Vollmers HP, Heider KH, et al. Importance of different CD44v6 expression in human gastric intestinal and diffuse type cancers for metastatic lymphogenic spreading [J].J Mol Med, 1995; 73(8)∶395.
- 9. Herrlich P, Pals S, Ponta H. CD44 in colon cancer [J].Eur J Cancer， 1995; 31A(7-8)∶1110.
- 10. Rodriguez C, Monges G, Rouanet P, et al.CD44 expression patterns in breast and colon tumors: a PCRbased study of splice variants [J]. Int J Cancer, 1995; 64(5)∶347.
- 11. Gunthert U,Hofmann M, Rudy W, et al. A new variant of glycoprotein CD44 confers metastatic potential to rat carcinoma cells [J]. Cell, 1991； 65(1)∶13.
- 12. Seiter S, Arch R, Reber S, et al. Prevention of tumor metastasis formation by antivariant CD44 [J]. J Exp Med, 1993; 177(2)∶443.
- 13. Hollstein M, Sidransky D,Vogelstein B,et al. p53 mutations in human cancers [J]. Science, 1991; 253(5015)∶49.
- 14. Merritt AJ, Potten CS,Kemp CJ, et al. The role of p53 in spontaneous and radiationinduced apoptosis in the gastrointestinal tract of normal and p53deficient mice [J]. Cancer Res, 1994; 54(3)∶614.
- 15. 武忠弼主編. 病理學(xué) [M].第4版.北京： 人民衛(wèi)生出版社，1997∶93～96.