• 1.中國醫(yī)學(xué)科學(xué)院中國協(xié)和醫(yī)科大學(xué)腫瘤醫(yī)院腫瘤研究所腹部外科（北京100021）;;
  • 2.大連醫(yī)科大學(xué)第一臨床學(xué)院普通外科（大連116011）;

目的觀察移植胰腺的腺泡細胞凋亡及其與急性排斥反應(yīng)的關(guān)系。方法選用SD和Wistar大鼠進行全胰十二指腸移植。實驗分為同基因移植組（WistarWistar）和異基因移植組（SDWistar）兩組。于術(shù)后第3 d、5 d和7 d分批處死受體，取移植胰腺標本用HE染色和原位末端標記（TUNEL）技術(shù)檢測移植胰腺切片，進行排斥反應(yīng)的病理學(xué)評分和計數(shù)凋亡指數(shù)（AI）。結(jié)果發(fā)生凋亡的細胞主要是腺泡細胞，同基因移植組胰腺有散在的腺泡細胞凋亡，AI在術(shù)后無明顯變化。異基因移植組胰腺腺泡細胞凋亡在術(shù)后第3 d、5 d和7 d逐漸升高，AI與急性排斥反應(yīng)的病理學(xué)評分成正相關(guān)。結(jié)論細胞凋亡與移植胰腺急性排斥反應(yīng)的嚴重程度顯著相關(guān)，凋亡指數(shù)可作為判斷移植物損傷程度的指標，對急性排斥反應(yīng)的診斷有一定的參考價值。

引用本文： 倪曉光, 關(guān)鳳林,劉忠,陳海龍,趙平,白曉楓. 腺泡細胞凋亡在大鼠胰腺移植急性排斥反應(yīng)中的意義. 中國普外基礎(chǔ)與臨床雜志, 2002, 9(6): 392-394. doi: 復(fù)制

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  2. 2. Gavrieli Y, Sherman Y, BenSassion SA. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation [Ｊ]. J Cell Biol, 1992; 119(3)∶493.
  3. 3. Lee S, Tung KS, Koopmans H, et al. Pancreaticoduodenal transplantation in the rat [Ｊ]. Transplantation, 1972; 13(4)∶421.
  4. 4. 孫治君，姚榛祥. 輸入供體血對延長大鼠移植胰腺功能存活的初步研究 [Ｊ]. 中國普外基礎(chǔ)與臨床雜志，2001； 8（6）∶364.
  5. 5. Nakhleh RE, Sutherland DE, Tzardis P, et al. Correlation of rejection of the duodenum with rejection of the pancreas in a pig model of pancreaticoduodenal transplantation [Ｊ]. Transplantation, 1993; 56(6)∶1353.
  6. 6. Shresta S, Pham CT, Thomas DA, et al. How do cytotoxic lymphocytes kill their targets? [Ｊ]. Curr Opin Immunol, 1998; 10(5)∶581.
  7. 7. Kagi D, Vignaux F, Ledermann B, et al. Fas and perforin pathways as the major mechanisms of T cellmediated cytotoxicity [Ｊ]. Science, 1994; 265(5171)∶528.