目的 探討端粒酶作為大腸癌診斷、治療和預(yù)后參數(shù)的價(jià)值。
方法 應(yīng)用PCR-TRAP-ELISA方法，檢測(cè)大腸癌、癌旁和正常大腸粘膜組織的端粒酶活性表達(dá)。
結(jié)果 端粒酶在大腸癌、癌旁和正常大腸粘膜組織中表達(dá)的陽性率分別為84.8%(39/46)、20.0%(6/30)和0(0/20)，大腸癌組織中端粒酶表達(dá)陽性率明顯高于癌旁和正常大腸粘膜(P＜0.001)。早期大腸癌(Dukes A期)即有66.7%者存在端粒酶活化。腫瘤組織學(xué)分級(jí)越低，端粒酶表達(dá)陽性率越高(P＜0.05)。
結(jié)論 端粒酶可能是大腸癌惡性浸潤(rùn)的早期事件，可作為診斷大腸癌的一個(gè)有用的輔助指標(biāo)，并有助于預(yù)測(cè)預(yù)后和指導(dǎo)治療方案的選擇。
引用本文： 駱成玉,趙丹寧,李世擁,祝學(xué)光. 檢測(cè)大腸癌組織中端粒酶活性的臨床意義. 中國(guó)普外基礎(chǔ)與臨床雜志, 2001, 8(1): 18-20. doi: 復(fù)制
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- 3. Kim NW, Pieatyszek MA, Prowse KR, et al. Specific association of human telomerase activity with immortal cells and cancer [J]. Science, 1994; 266(5232)∶2011.
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