• 西安醫(yī)科大學(xué)第二附屬醫(yī)院病理科(西安 710004);

目的  觀察細胞周期素D1、Rb及p16在早期胃癌發(fā)生中的作用及相互關(guān)系。
方法  采用免疫組化SP法對39例早期胃癌癌旁腸化組織及34例非癌胃粘膜腸化組織進行對比研究。
結(jié)果  癌組織中33/39例(84.6%)顯示細胞周期素D1過度表達，癌旁腸化組織也顯示細胞周期素D1過度表達。12/39例p16表達陰性，3例呈弱陽性。26例Rb陽性的腫瘤中，15例顯示p16不表達或低表達，而9例Rb陰性的腫瘤中則p16顯示表達增高。
結(jié)論  細胞周期素D1是早期胃癌發(fā)生過程中常見的分子異常，細胞周期素D1的激活及Rb的失活在早期胃癌中可共同存在；Rb失活與p16表達之間存在負相關(guān)關(guān)系；早期胃癌的發(fā)生可能與細胞周期素D1、p16及Rb負反饋調(diào)節(jié)環(huán)路的異常有關(guān)。

引用本文： 陳曉黎,王康敏,蘇寶山,孫潤芹,黃鶯,強雷. 細胞周期素D1、Rb及p16在早期胃癌中的表達及意義. 中國普外基礎(chǔ)與臨床雜志, 2001, 8(1): 29-31. doi: 復(fù)制

1. Constancia M, Seruca R, Carneiro F, et al. Retinoblatoma gene structure and product in human gastric carcinoma[J]. Br J Cancer, 1994; 70(5)∶1018.
2. Igaki H, Sasaki H, Tachimori Y, et al. Mutation frequency of the p16/CDKN2 gene in primary cancers in the upper digestive tract[J]. Cancer Res, 1995; 55(15)∶3421.
3. Sakata K, Tamura G, Maesawa C, et al. Loss of heterozygosity on the short arm of chromosome 9 without p16 gene mutation in gastric carcinoma [J]. Jan J Cancer Res, 1995; 86(4)∶333.
4. Akama Y, Yasui W, Kuniyasu H, et al. Genetic status and expression of the cyclin depent kinase inhibitor in human gastric carcinoma cell lines [J]. Jan J Cancer Res, 1996; 87(8)∶824.
5. 呂有勇，高崇峰，崔建濤等.胃癌中MTS1/p16基因缺失及表達異常的研究 [J].中華腫瘤雜志，1996； 18(3)∶442.
6. 鄧仲端，陳學(xué)詩，彭杰青等.外科病理學(xué) [M].武漢：湖北科技出版社，1978∶87～88.
7. Gillett C, Fant V, Bartek J, et al. Amplification and overexpression of cyclin D1 in breast cancer detected by immunohistochemical staining [J]. Cancer Res, 1994; 54(9)∶1812.
8. Shapiro GI, Edwards CD, Kobzik L, et al. Reciprocal Rb inactivation and p16 INK4 expression in primary lung cancer and cell lines[J]. Cancer Res, 1995; 55(3)∶505.
9. Sakaguchi M, Fujii, Hirabayashi H, et al. Inversely correlated expression of p16 and Rb protein in nonsmall cell lung cancers:an immunohistochemical study [J]. Int J Cancer, 1996; 65(3)∶442.
10. Yeager T, Studler W, Belair C, et al. Increased p16 levels correlate with Rb alteratious in human urothelial cell [J]. Cancer Res, 1995; 55(3)∶493.
11. 藺玉昌，黃長銘，劉道坤等.星形細胞瘤p16與Rb蛋白的表達及其相關(guān)性研究 [J].中華病理雜志，1997； 26(6)∶340.
  1. 1. Constancia M, Seruca R, Carneiro F, et al. Retinoblatoma gene structure and product in human gastric carcinoma[J]. Br J Cancer, 1994; 70(5)∶1018.
  2. 2. Igaki H, Sasaki H, Tachimori Y, et al. Mutation frequency of the p16/CDKN2 gene in primary cancers in the upper digestive tract[J]. Cancer Res, 1995; 55(15)∶3421.
  3. 3. Sakata K, Tamura G, Maesawa C, et al. Loss of heterozygosity on the short arm of chromosome 9 without p16 gene mutation in gastric carcinoma [J]. Jan J Cancer Res, 1995; 86(4)∶333.
  4. 4. Akama Y, Yasui W, Kuniyasu H, et al. Genetic status and expression of the cyclin depent kinase inhibitor in human gastric carcinoma cell lines [J]. Jan J Cancer Res, 1996; 87(8)∶824.
  5. 5. 呂有勇，高崇峰，崔建濤等.胃癌中MTS1/p16基因缺失及表達異常的研究 [J].中華腫瘤雜志，1996； 18(3)∶442.
  6. 6. 鄧仲端，陳學(xué)詩，彭杰青等.外科病理學(xué) [M].武漢：湖北科技出版社，1978∶87～88.
  7. 7. Gillett C, Fant V, Bartek J, et al. Amplification and overexpression of cyclin D1 in breast cancer detected by immunohistochemical staining [J]. Cancer Res, 1994; 54(9)∶1812.
  8. 8. Shapiro GI, Edwards CD, Kobzik L, et al. Reciprocal Rb inactivation and p16 INK4 expression in primary lung cancer and cell lines[J]. Cancer Res, 1995; 55(3)∶505.
  9. 9. Sakaguchi M, Fujii, Hirabayashi H, et al. Inversely correlated expression of p16 and Rb protein in nonsmall cell lung cancers:an immunohistochemical study [J]. Int J Cancer, 1996; 65(3)∶442.
  10. 10. Yeager T, Studler W, Belair C, et al. Increased p16 levels correlate with Rb alteratious in human urothelial cell [J]. Cancer Res, 1995; 55(3)∶493.
  11. 11. 藺玉昌，黃長銘，劉道坤等.星形細胞瘤p16與Rb蛋白的表達及其相關(guān)性研究 [J].中華病理雜志，1997； 26(6)∶340.