• 華西醫(yī)科大學(xué)附屬第一醫(yī)院普外科(成都610041);

目的  探討重組腺病毒相關(guān)病毒(rAAV)的制備以及作為肝癌基因治療載體的可行性。
方法  重組報(bào)告基因加強(qiáng)的綠色熒光蛋白(EGFP)基因至質(zhì)粒載體pSub201，用雙質(zhì)粒共轉(zhuǎn)染方法制備重組腺病毒相關(guān)病毒顆粒(rAAV/EGFP)，觀察其對(duì)肝癌細(xì)胞系的感染性。
結(jié)果  用常規(guī)的雙質(zhì)粒共轉(zhuǎn)染方法能制備高產(chǎn)量的rAAV(107～108感染單位/10 cm板)； 當(dāng)病毒感染多重性(MOI)=100時(shí)，rAAV/EGFP對(duì)肝癌細(xì)胞HepG2的轉(zhuǎn)染效率可達(dá)100%。
結(jié)論  重組腺病毒相關(guān)病毒可有效地轉(zhuǎn)導(dǎo)外源基因至肝癌細(xì)胞，其作為肝癌的基因治療載體具有較好的研究前景。

引用本文： 劉自明,嚴(yán)律南,王忠,林琦遠(yuǎn). 重組腺病毒相關(guān)病毒作為肝癌基因治療載體的初步研究. 中國(guó)普外基礎(chǔ)與臨床雜志, 2001, 8(3): 133-134. doi: 復(fù)制

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  3. 3. Maass G, Bogedain C, Scheer U, et al. Recombinant adenoassociated virus for the generation of autologous, genemodified tumor vaccine:evidence for a high transduction efficiency into primary epithelial cancer cells 〔J〕. Human Gene Therapy, 1998； 9(7)∶1049.
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