• 上海醫(yī)科大學中山醫(yī)院肝癌研究所（上海200032）;

目的  評價DNA含量和肝細胞肝癌臨床生物學特性的關(guān)系以及在提示復發(fā)中的價值。
方法  對140例肝癌新鮮肝切除標本DNA含量用流式細胞技術(shù)進行分析，同時收集患者的臨床生物學特性資料，并且隨訪患者腫瘤復發(fā)情況。
結(jié)果  DNA倍體類型和患者年齡相關(guān)（Ｐ＜0.05），和血清AFP水平、腫瘤的大小密切相關(guān)（Ｐ＜0.01）。二倍體肝癌術(shù)后平均隨訪31.2個月，其術(shù)后復發(fā)率為23.1%，1年復發(fā)率為4.3%； 異倍體肝癌平均隨訪時間為22.6個月，術(shù)后復發(fā)率為50.0%，術(shù)后1年復發(fā)率為37.9%。兩者復發(fā)差異的Ｐ=0.013，術(shù)后1年復發(fā)差異的Ｐ=0.002。多因素回歸分析表明，患者肝硬變程度、腫瘤大小及DNA含量為提示肝癌復發(fā)獨立因素。
結(jié)論  DNA含量增加可以作為提示肝癌高復發(fā)的獨立因素。

引用本文： 溫險峰,馬曾辰,林芷英,葉青海,湯釗猷. 肝細胞肝癌中DNA含量分析提示復發(fā)的價值Logistic回歸. 中國普外基礎(chǔ)與臨床雜志, 2001, 8(4): 236-238. doi: 復制

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6. Fujimoto J, Okamoto E, Yamanaka N, et al. Flow cytometric DNA analysis of hepatocellular carcinoma 〔Ｊ〕. Cancer, 1991; 67(4)∶939.
7. Hedley DW, Friedlander ML, Talor IW. Application of DNA flow cytometry to paraffinembedded archival meterial for the study of aneuploidy and its clinical significance 〔Ｊ〕. Cytometry, 1985; 6(3)∶327.
8. Hagg D, Feichter G, Goerttler K, et al. Influence of systematic errors on the evaluation of the Sphase portions from DNA distributions of solid tumors as showns for 328 breast carcinomas 〔Ｊ〕. Cytometry, 1987; 8(3)∶377.
9. Nagasue N,Yamanoi A, Takemoto Y, et al. Comparison between diploid and aneuploid hepatocellular carcinomas: A flow cytometric study 〔Ｊ〕. Br J Surg, 1992; 79(7)∶667.
10. Kuo SH, Sheu JC, Chen DS, et al. Cytometric measurements of nuclear DNA content in hepatocellular carcinoma 〔Ｊ〕. Hepatology, 1987; 7(2)∶330.
11. Kute TE, Gregory B, Galleshaw J, et al. How reproducible are flow cytometry data from paraffinembedded blocks 〔Ｊ〕? Cytometry, 1988; 9(4)∶494.
12. Auer GU, Fallenius AG, Erhardt KY, et al. Progression of mammary adenocarcinoma as reflected by nuclear DNA content 〔Ｊ〕. Cytometry, 1984; 5(4)∶420.
13. 湯釗猷主編. 現(xiàn)代腫瘤學 〔M〕. 上海: 上海醫(yī)科大學出版社, 1993∶553~585.
  1. 1. Farmer DC, Rosove MH, Shaked A, et al. Current treatment modalities for hepatocellular carcinoma 〔Ｊ〕. Ann Surg, 1994; 219(3)∶236.
  2. 2. Tang ZY. Small hepatocellular carcinoma 〔M〕. In: Tang ZY, Wu MC, Xia SS(eds). Primary Liver Cancer. Berlin: Springer, 1989∶191-203.
  3. 3. Merkel DE, Mcguire WL. Ploidy, proliferative activity and prognosis DNA flow cytometry of solid tumors 〔Ｊ〕. Cancer, 1990; 65(4)∶1194.
  4. 4. Ng IO, Lai EC, Ho JC, et al. Flow cytometric analysis of DNA ploidy in hepatocellular carcinoma 〔Ｊ〕. Am J Clin pathol, 1994; 102(1)∶80.
  5. 5. Hamazaki K, Kato T, Yunoki Y, et al. An analysis of DNA ploidy pattern of hepatocellular carcinoma 〔Ｊ〕. Acta Med Okayama, 1993; 47(6)∶413.
  6. 6. Fujimoto J, Okamoto E, Yamanaka N, et al. Flow cytometric DNA analysis of hepatocellular carcinoma 〔Ｊ〕. Cancer, 1991; 67(4)∶939.
  7. 7. Hedley DW, Friedlander ML, Talor IW. Application of DNA flow cytometry to paraffinembedded archival meterial for the study of aneuploidy and its clinical significance 〔Ｊ〕. Cytometry, 1985; 6(3)∶327.
  8. 8. Hagg D, Feichter G, Goerttler K, et al. Influence of systematic errors on the evaluation of the Sphase portions from DNA distributions of solid tumors as showns for 328 breast carcinomas 〔Ｊ〕. Cytometry, 1987; 8(3)∶377.
  9. 9. Nagasue N,Yamanoi A, Takemoto Y, et al. Comparison between diploid and aneuploid hepatocellular carcinomas: A flow cytometric study 〔Ｊ〕. Br J Surg, 1992; 79(7)∶667.
  10. 10. Kuo SH, Sheu JC, Chen DS, et al. Cytometric measurements of nuclear DNA content in hepatocellular carcinoma 〔Ｊ〕. Hepatology, 1987; 7(2)∶330.
  11. 11. Kute TE, Gregory B, Galleshaw J, et al. How reproducible are flow cytometry data from paraffinembedded blocks 〔Ｊ〕? Cytometry, 1988; 9(4)∶494.
  12. 12. Auer GU, Fallenius AG, Erhardt KY, et al. Progression of mammary adenocarcinoma as reflected by nuclear DNA content 〔Ｊ〕. Cytometry, 1984; 5(4)∶420.
  13. 13. 湯釗猷主編. 現(xiàn)代腫瘤學 〔M〕. 上海: 上海醫(yī)科大學出版社, 1993∶553~585.