目的 研究肝細胞肝癌細胞中增殖細胞核抗原（PCNA）/凋亡細胞比值與肝細胞肝癌分化程度的關(guān)系。
方法 應(yīng)用原位末端標記法（TUNEL）及免疫組化雙染色技術(shù)，對35例肝細胞肝癌石蠟包埋組織進行檢測。
結(jié)果 不同分化程度癌組織中細胞凋亡和PCNA的陽性率不同。分化程度高，細胞凋亡陽性率高、PCNA陽性率偏低； 分化程度低，細胞凋亡陽性率偏低、PCNA陽性率高。隨分化程度的降低，PCNA/凋亡細胞的比值顯著增加，二者有良好的相關(guān)性。
結(jié)論 PCNA/凋亡細胞的比值可作為評估肝細胞肝癌分化程度的指標。
引用本文： 何勇,周峻,竇科峰. 細胞凋亡及增殖細胞核抗原雙染色在肝細胞肝癌組織中的研究. 中國普外基礎(chǔ)與臨床雜志, 2001, 8(4): 249-251. doi: 復(fù)制
| 1. | Edmondson HA. Primary carcinoma of the liver: a study of 100 cases among 48 900 necropsies 〔Ｊ〕. Cancer, 1954; 7(9)∶642. |
| 2. | 鄭德先. 細胞凋亡的研究進展 〔Ｊ〕. 中華病理學(xué)雜志， 1996； 25（1）∶50. |
| 3. | Willims GT, Smith CA. Molecular regulation of apoptosis: genetic controls on cell death 〔Ｊ〕. Cell, 1993; 74(3)∶777. |
| 4. | Miyachi K. Autoantibody to a nuclear antigen in proliferating cells 〔Ｊ〕. Journal of Immunology, 1978; 121(10)∶2228. |
| 5. | Mathews MB, Bernstein RM, Franza BR Jr, et al. Identity of the proliferating cell nuclear antigen and cyclin 〔Ｊ〕. Nature, 1984; 309(5966)∶374. |
| 6. | Tuusa J, Uitto L, Syvaoja JE, Human DNA polymerase epsilon is expressed during cell proliferation in a manner characteristic of replicative DNA polymerases 〔Ｊ〕. Nucleic Acid Res, 1995; 23(12)∶2178. |
| 7. | Irene OL, Edward CS, Sheung T, et al. Prognostic significance of proliferating cell nuclear antigen expression in hepatocellular carcinoma 〔Ｊ〕. Cancer, 1994; 73(9)∶2268. |
| 8. | Soini Y, Virkajarvi N, Lehto VP, et al. Hepatocellular carcinomas with a high proliferation index and a low degree of apoptosis and necrosis are associated with a shorted survival 〔Ｊ〕. Br J Cancer, 1996; 73(4)∶1025. |
| 9. | Hino N, Higashi T, Nouso K, et al. Apoptosis and proliferation of human hepatocellular carcinoma 〔Ｊ〕. Liver, 1996; 16(2)∶123. |
- 1. Edmondson HA. Primary carcinoma of the liver: a study of 100 cases among 48 900 necropsies 〔Ｊ〕. Cancer, 1954; 7(9)∶642.
- 2. 鄭德先. 細胞凋亡的研究進展 〔Ｊ〕. 中華病理學(xué)雜志， 1996； 25（1）∶50.
- 3. Willims GT, Smith CA. Molecular regulation of apoptosis: genetic controls on cell death 〔Ｊ〕. Cell, 1993; 74(3)∶777.
- 4. Miyachi K. Autoantibody to a nuclear antigen in proliferating cells 〔Ｊ〕. Journal of Immunology, 1978; 121(10)∶2228.
- 5. Mathews MB, Bernstein RM, Franza BR Jr, et al. Identity of the proliferating cell nuclear antigen and cyclin 〔Ｊ〕. Nature, 1984; 309(5966)∶374.
- 6. Tuusa J, Uitto L, Syvaoja JE, Human DNA polymerase epsilon is expressed during cell proliferation in a manner characteristic of replicative DNA polymerases 〔Ｊ〕. Nucleic Acid Res, 1995; 23(12)∶2178.
- 7. Irene OL, Edward CS, Sheung T, et al. Prognostic significance of proliferating cell nuclear antigen expression in hepatocellular carcinoma 〔Ｊ〕. Cancer, 1994; 73(9)∶2268.
- 8. Soini Y, Virkajarvi N, Lehto VP, et al. Hepatocellular carcinomas with a high proliferation index and a low degree of apoptosis and necrosis are associated with a shorted survival 〔Ｊ〕. Br J Cancer, 1996; 73(4)∶1025.
- 9. Hino N, Higashi T, Nouso K, et al. Apoptosis and proliferation of human hepatocellular carcinoma 〔Ｊ〕. Liver, 1996; 16(2)∶123.