• 1.研究生,現(xiàn)在湖南省湘潭市中心醫(yī)院普外科(湘潭411200);;
  • 2.重慶醫(yī)科大學(xué)附屬第一醫(yī)院普外科(重慶400016);

目的  探討槲皮素抑制乳腺癌發(fā)生及增殖的作用。方法  建立二甲基苯丙蒽(DMBA)誘導(dǎo)的乳腺癌動(dòng)物模型。79只雌性SD大鼠隨機(jī)分為A組(DMBA)、B組〔DMBA+三苯氧胺(TAM)〕、C組(DMBA+槲皮素)及D組(空白對(duì)照)。持續(xù)喂養(yǎng)28周,經(jīng)光鏡、電鏡觀察,抗PCNA及Hras免疫組化分析。結(jié)果  ①A組大鼠乳腺腫瘤發(fā)生率為76.2%,明顯高于B組(40.9%)、C組(45.5%)及D組(0%),P<0.05; B組與C組相比差異無(wú)顯著性意義(P>0.05)。②A組大鼠乳腺癌平均直徑2.37cm,明顯大于B組(1.82cm)及C組(1.71cm),P<0.05; B組和C組相比差異無(wú)顯著性意義(P>0.05)。③PCNA免疫組化染色顯示: A組與B組和C組相比差異有顯著性意義(P<0.05),B組和C組相比差異無(wú)顯著性意義(P>0.05)。④Hras免疫組化染色顯示: A組與B組和C組比較差異有顯著性意義(P<0.05),B組和C組相比差異無(wú)顯著性意義(P>0.05)。結(jié)論  槲皮素有降低DMBA誘導(dǎo)的SD大鼠乳腺癌發(fā)生率及抑制腫瘤生長(zhǎng)的作用,其機(jī)理可能與抑制ras基因活性、阻抑細(xì)胞增殖有關(guān)。

引用本文: 林輝,吳凱南,孔令泉. 槲皮素抑制乳腺癌發(fā)生及增殖的實(shí)驗(yàn)研究. 中國(guó)普外基礎(chǔ)與臨床雜志, 2001, 8(5): 288-291. doi: 復(fù)制

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1. 孔令泉,吳凱南. 槲皮素抗腫瘤作用研究進(jìn)展 〔J〕. 四川醫(yī)學(xué),1999; 20(1)∶52.
2. Lu HA, Niggenmann B, Zanker KS, et al. Suppression of the proliferation and migration of oncogenic rasdependent cell linescultured in threedimensional collogen matrix by flavonoidstructure molecules 〔J〕. J Cell Res Clin Oncol,1996; 122(6)∶335.
3. Steven R, Donna S, Dieffenbach A, et al. Prediction of early relapse and shortened survived in patients with breast cancer by proliferating cell nuclear antigen score 〔J〕. Cancer, 1993; 71(19)∶3552.
4. 甘長(zhǎng)清,吳凱南. 乳康片抑制實(shí)驗(yàn)性乳腺增生癥及其機(jī)理的研究 〔J〕. 中國(guó)腫瘤臨床,1999; 26(增刊)∶111.
5. 高進(jìn)主編. 腫瘤學(xué)基礎(chǔ)與實(shí)驗(yàn) 〔M〕. 第1版. 北京: 北京醫(yī)科大學(xué)、中國(guó)協(xié)和醫(yī)科大學(xué)聯(lián)合出版社, 1992∶25~26.
6. 吳小華,蔡樹(shù)模,張志毅等. 卵巢腫瘤中癌基因nm23H1,Cerb B2, ras和p53的蛋白表達(dá)與腹膜后淋巴結(jié)轉(zhuǎn)移關(guān)系的研究 〔J〕. 腫瘤,1997; 17(3)∶134.
7. Ajik KV, Jeffrey AJ, Micheel NG, et al. Inhibition of 7,12dimethylbenz(a) anthraceneand Nnitrosomethylureainduced rat mammary cancer by dietay flavonol quercetin 〔J〕. Cancer Res,1998; 48(25)∶5754.
8. 張繼增,劉華福,劉斌等. 乳腺增生病組織學(xué)分類及其與乳腺癌分類的研究 〔J〕. 中華病理學(xué)雜志,1995; 24(1)∶43.
9. 李樹(shù)玲,劉奇,于金明等主編. 乳腺癌研究進(jìn)展 〔C〕. 第二屆國(guó)際乳腺癌學(xué)術(shù)會(huì)議論文匯編. 濟(jì)南: 濟(jì)南出版社,1996∶153~155.
10. Larcher F, Robles AI, Duran H. Up regulation of vascular endothelial growth factor/vascular permeability factor in mouse skin carcinogenesis correlates with malignant progression and activated Hras expression levels 〔J〕. Cancer Res, 1996; 56(23)∶5391.
11. 張帆,張乃鑫. 大鼠化學(xué)誘發(fā)性乳癌的形態(tài)發(fā)生及其生物標(biāo)記的研究狀況 〔J〕. 中國(guó)腫瘤臨床,1999; 26(1)∶64.
12. Avila MA, Cansado J, Harter KV, et al. Quercetin as a modulator of the cellular neoplastic phenotype: effect on the expression of mutated Hras and p53 in rodent and human cells 〔J〕. Adv Exp Med Biol, 1996; 401(1)∶ 101.
13. Csokay B, Prajda N, Weber G, et al. Molecular mechanism in the antiproliferative action of quercetin 〔J〕. Life Sci, 1997; 60(24)∶ 2157.
  1. 1. 孔令泉,吳凱南. 槲皮素抗腫瘤作用研究進(jìn)展 〔J〕. 四川醫(yī)學(xué),1999; 20(1)∶52.
  2. 2. Lu HA, Niggenmann B, Zanker KS, et al. Suppression of the proliferation and migration of oncogenic rasdependent cell linescultured in threedimensional collogen matrix by flavonoidstructure molecules 〔J〕. J Cell Res Clin Oncol,1996; 122(6)∶335.
  3. 3. Steven R, Donna S, Dieffenbach A, et al. Prediction of early relapse and shortened survived in patients with breast cancer by proliferating cell nuclear antigen score 〔J〕. Cancer, 1993; 71(19)∶3552.
  4. 4. 甘長(zhǎng)清,吳凱南. 乳康片抑制實(shí)驗(yàn)性乳腺增生癥及其機(jī)理的研究 〔J〕. 中國(guó)腫瘤臨床,1999; 26(增刊)∶111.
  5. 5. 高進(jìn)主編. 腫瘤學(xué)基礎(chǔ)與實(shí)驗(yàn) 〔M〕. 第1版. 北京: 北京醫(yī)科大學(xué)、中國(guó)協(xié)和醫(yī)科大學(xué)聯(lián)合出版社, 1992∶25~26.
  6. 6. 吳小華,蔡樹(shù)模,張志毅等. 卵巢腫瘤中癌基因nm23H1,Cerb B2, ras和p53的蛋白表達(dá)與腹膜后淋巴結(jié)轉(zhuǎn)移關(guān)系的研究 〔J〕. 腫瘤,1997; 17(3)∶134.
  7. 7. Ajik KV, Jeffrey AJ, Micheel NG, et al. Inhibition of 7,12dimethylbenz(a) anthraceneand Nnitrosomethylureainduced rat mammary cancer by dietay flavonol quercetin 〔J〕. Cancer Res,1998; 48(25)∶5754.
  8. 8. 張繼增,劉華福,劉斌等. 乳腺增生病組織學(xué)分類及其與乳腺癌分類的研究 〔J〕. 中華病理學(xué)雜志,1995; 24(1)∶43.
  9. 9. 李樹(shù)玲,劉奇,于金明等主編. 乳腺癌研究進(jìn)展 〔C〕. 第二屆國(guó)際乳腺癌學(xué)術(shù)會(huì)議論文匯編. 濟(jì)南: 濟(jì)南出版社,1996∶153~155.
  10. 10. Larcher F, Robles AI, Duran H. Up regulation of vascular endothelial growth factor/vascular permeability factor in mouse skin carcinogenesis correlates with malignant progression and activated Hras expression levels 〔J〕. Cancer Res, 1996; 56(23)∶5391.
  11. 11. 張帆,張乃鑫. 大鼠化學(xué)誘發(fā)性乳癌的形態(tài)發(fā)生及其生物標(biāo)記的研究狀況 〔J〕. 中國(guó)腫瘤臨床,1999; 26(1)∶64.
  12. 12. Avila MA, Cansado J, Harter KV, et al. Quercetin as a modulator of the cellular neoplastic phenotype: effect on the expression of mutated Hras and p53 in rodent and human cells 〔J〕. Adv Exp Med Biol, 1996; 401(1)∶ 101.
  13. 13. Csokay B, Prajda N, Weber G, et al. Molecular mechanism in the antiproliferative action of quercetin 〔J〕. Life Sci, 1997; 60(24)∶ 2157.