目的 探索山莨菪堿對大鼠肝臟缺血再灌注損傷的保護(hù)作用。方法 選雄性Wistar大鼠160只，分為正常對照組、缺血再灌注組、生理鹽水組和山莨菪堿組，觀察了肝臟缺血60分鐘及再灌注1、3、6、12及24小時(shí)后血漿內(nèi)皮素-1（ET-1）、透明質(zhì)酸（HA）和谷丙轉(zhuǎn)氨酶（ALT）含量變化及肝組織病理學(xué)變化。結(jié)果 肝臟缺血再灌注后，血漿ET-1、HA和ALT含量均顯著增高，同時(shí)肝臟瘀血很明顯； 肝臟缺血再灌注前用山莨菪堿后，血漿HA和ALT含量明顯降低，同時(shí)肝組織瘀血減輕。結(jié)論 山莨菪堿可改善再灌注后的肝臟微循環(huán)障礙，對大鼠肝臟缺血再灌注損傷有保護(hù)作用。
引用本文： 董滿庫,崔彥,陳昌瑋,王平,肖廣生,周立艷,李曉鷗,吉敏,劉子沛. 山莨菪堿對大鼠肝臟缺血再灌注損傷保護(hù)作用的實(shí)驗(yàn)研究. 中國普外基礎(chǔ)與臨床雜志, 2001, 8(5): 295-297. doi: 復(fù)制
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| 11. | Wang WC, Lee YN. Effects of anisodamine and atropine on the microvasculature of liver, skeletal muscle and footpad skin in anesthetized rats 〔Ｊ〕. Yao Hsueh Hsueh Pao, 1992; 27(5)∶385. |
- 1. Nauta RJ, Tsimoyiannis E, Urib M, et al. Oxygenderived free radical in hepatic ischemia reperfusion injury in rat 〔Ｊ〕. Surg Gynecol Obstet, 1990; 171(2)∶120.
- 2. Yanagisawa M, Kurihara H, Kimuras E, et al. A novel potent vasoconstrictor peptide produced by vascular endothelial cells 〔Ｊ〕. Nature, 1988; 332(6163)∶411.
- 3. Itasaka H, Kishikawa K, Suehiro T, et al. Serum hyaluronic acid for the assessment of graft viability in porcine liver transplantation 〔Ｊ〕. Surg Today, 1994; 24(8)∶719.
- 4. Zhang JX, Pegoli W, Clemens MG, et al. Endothelin1 induces direct constriction of hepatic sinusoids 〔Ｊ〕. Am J Physiol, 1994; 266(29)∶G624.
- 5. Mitsuoka H, Suzuki S, Sakaguchi T, et al. Contribution of endothelin1 to microcirculatory impairment in total hepatic ischemia and reperfusion injury 〔Ｊ〕. Transplantation, 1999; 67(4)∶514.
- 6. Wang Y, Lawson JA, Jaeschke H. Differential effect of 2aminoethylisothiourea, an inhibitor of the inducible nitric oxide synthase, on microvascular blood flow and organ injury in models of hepatic ischemiareperfusion and endotoxemia 〔Ｊ〕. Shock, 1998; 10(1)∶20.
- 7. Menger MD, Richter S, Yamauchi J, et al. Role of microcirculation in hepatic ischemia/reperfusion injury 〔Ｊ〕. Hepatogastroenterology, 1999; 46(12)∶1452.
- 8. Cursio R, Gugenheim J, PanaiaFerrari P, et al. Improvement of normothermic rat liver ischemia/reperfusion by muramyl dipeptide 〔Ｊ〕. J Surg Res, 1998; 80(2)∶339.
- 9. Soejima Y, Yanaga K, Nishizaki T, et al. Effect of specific neutrophil elastase inhibitor on ischemia/reperfusion injury in rat liver transplantation 〔Ｊ〕. J Surg Res, 1999; 86(1)∶150.
- 10. 邢卉春, 趙龍鳳, 王守義. 山莨菪堿對大鼠急性肝損傷的保護(hù)作用及其機(jī)制 〔Ｊ〕. 中國危重病急救醫(yī)學(xué), 1998; 10(11)∶658.
- 11. Wang WC, Lee YN. Effects of anisodamine and atropine on the microvasculature of liver, skeletal muscle and footpad skin in anesthetized rats 〔Ｊ〕. Yao Hsueh Hsueh Pao, 1992; 27(5)∶385.