高軍 1 , 張淑文 2 , 李非 2
  • 1.北京市第六醫(yī)院外科（北京100007）;;
  • 2.北京市宣武醫(yī)院普外科（北京100053）;

目的  探討大鼠實驗性急性胰腺炎（AP）模型中胰蛋白酶原激活肽（TAP）的產(chǎn)生及意義。 方法  將90只SD大鼠隨機分為5組： EP組（3%?；悄懰徕c逆行膽胰管注射組）； NP組（5%?；悄懰徕c逆行膽胰管注射組）； TP組（3%?；悄懰徕c逆行膽胰管注射后半小時經(jīng)股靜脈注入烏司他丁組）； CP組（0.9%生理鹽水逆行膽胰管注射組）； OP組（假手術(shù)組）。分別于制模后3、6及24小時后處死動物，取血測定血淀粉酶和TAP水平，同時取胰腺標本觀察其組織病理學改變并評分。結(jié)果  NP組胰腺病變明顯重于EP組。制模后3小時和6小時血漿TAP水平NP組分別為（4.798±0.169）nmol/L和(3.999±0.299)nmol/L，明顯高于EP組的（2.416±0.148）nmol/L和(3.356±0.211)nmol/L； 在制模6小時后TP組血漿TAP水平為（1.611±0.113）nmol/L，比EP組的(3.356±0.211)nmol/L明顯降低。EP組與NP組血漿TAP水平差異的出現(xiàn)早于兩組間胰腺組織病理學改變差異的出現(xiàn)。結(jié)論  血漿TAP水平與大鼠實驗性AP的嚴重程度有關(guān)。血漿TAP水平可以作為早期預(yù)測實驗性AP嚴重程度的指標。

引用本文： 高軍,張淑文,李非. 胰蛋白酶原激活肽在大鼠實驗性急性胰腺炎中的產(chǎn)生及意義. 中國普外基礎(chǔ)與臨床雜志, 2001, 8(5): 301-303下轉(zhuǎn)306. doi: 復制

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2. Paul R,Alistair C,Ahmed J, et al. Development of radioimmunoassays for free tetraLaspartylLlysine trypsinogen activation peptides 〔Ｊ〕. J Immunol Meth,1988； 111(2)∶195.
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4. Aho HJ, Koskensalo ML, Nevalainen TJ. Experimental pancreatitisin the rat: sodium taurocholateinduced acute haemorrhagic pancreatitis 〔Ｊ〕. Scand J Gastroenterol, 1980； 15(4)∶411.
5. Schmidt J, Lewandrowsi K,Warshaw AL, et al. Morphometric characteristics and homogeneity of a new model of acute pancreatitis in the rat 〔Ｊ〕. Int J Pancreatol,1992； 12(1)∶41.
6. Pozsar J, Berger Z, Simon K, et al. Biphasic effect of prostaglandin E1 on the severity of acute pancreatitis induced by a closed duodenal loop in rats 〔Ｊ〕. Pancreas, 1996； 12(2)∶159.
7. Wang Youxue,Satoru N,Motoji K, et al. Do plasma and urine trypsinogen activation peptides(TAP) really increase in trypsintaurocholateinduced pancreatitis 〔Ｊ〕. Pancreas, 2000； 20(4)∶389.
8. Yasuyuki Nakae, Satoru Naruse, Motoji Kitagawa, et al. Activation of trypsinogen in experimental model of acute pancreatitis in rats 〔Ｊ〕. Pancreas, 1995； 10(3)∶306.
9. Kai M, Casitillo CF,Frick TW, et al. Increased intrapancreatic trypsinogen activation in ischemiainduced experimental pancreatitis 〔Ｊ〕. Ann Surg, 1995； 221(4)∶364.
10. Simpson KW,Bvmts N,Beechey N, et al. Cholecystokinin8 induces edematous pancreatitis in dogs associated with short burst of trypsinogen activation 〔Ｊ〕. Dig Dis Sci, 1995； 40(10)∶2152.
  1. 1. Gudmundsdottir A,Gudmundsdottir E,Oskarsson S,et al. Isolation and characterization of cDNAs from Atlantic cod encoding two different forms of trypsinogen 〔Ｊ〕. Eur J Biochem,1993; 217(3)∶1091.
  2. 2. Paul R,Alistair C,Ahmed J, et al. Development of radioimmunoassays for free tetraLaspartylLlysine trypsinogen activation peptides 〔Ｊ〕. J Immunol Meth,1988； 111(2)∶195.
  3. 3. Carlos fernandezdel C,Schmidt J,David W,et al. Generation and possible significance of trypsinogen activation peptide in experimental acute pancreatitis in rat 〔Ｊ〕. Pancreas,1992； 7(3)∶263.
  4. 4. Aho HJ, Koskensalo ML, Nevalainen TJ. Experimental pancreatitisin the rat: sodium taurocholateinduced acute haemorrhagic pancreatitis 〔Ｊ〕. Scand J Gastroenterol, 1980； 15(4)∶411.
  5. 5. Schmidt J, Lewandrowsi K,Warshaw AL, et al. Morphometric characteristics and homogeneity of a new model of acute pancreatitis in the rat 〔Ｊ〕. Int J Pancreatol,1992； 12(1)∶41.
  6. 6. Pozsar J, Berger Z, Simon K, et al. Biphasic effect of prostaglandin E1 on the severity of acute pancreatitis induced by a closed duodenal loop in rats 〔Ｊ〕. Pancreas, 1996； 12(2)∶159.
  7. 7. Wang Youxue,Satoru N,Motoji K, et al. Do plasma and urine trypsinogen activation peptides(TAP) really increase in trypsintaurocholateinduced pancreatitis 〔Ｊ〕. Pancreas, 2000； 20(4)∶389.
  8. 8. Yasuyuki Nakae, Satoru Naruse, Motoji Kitagawa, et al. Activation of trypsinogen in experimental model of acute pancreatitis in rats 〔Ｊ〕. Pancreas, 1995； 10(3)∶306.
  9. 9. Kai M, Casitillo CF,Frick TW, et al. Increased intrapancreatic trypsinogen activation in ischemiainduced experimental pancreatitis 〔Ｊ〕. Ann Surg, 1995； 221(4)∶364.
  10. 10. Simpson KW,Bvmts N,Beechey N, et al. Cholecystokinin8 induces edematous pancreatitis in dogs associated with short burst of trypsinogen activation 〔Ｊ〕. Dig Dis Sci, 1995； 40(10)∶2152.