• 1. 華西醫(yī)科大學(xué)附屬第一醫(yī)院普外科(成都610041)2. 華西醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院生化教研室;

為研究兔膽囊結(jié)石成石過(guò)程中血漿脂蛋白膽固醇變化，及其對(duì)膽汁中膽汁酸、膽固醇含量及成石的影響，在用高膽固醇膳食(HCD)致兔膽囊膽固醇結(jié)石模型基礎(chǔ)上，將動(dòng)物分為5組，每組10只，動(dòng)態(tài)監(jiān)測(cè)成石過(guò)程中血漿脂蛋白膽固醇、總膽固醇(TC)、磷脂、甘油三酯、膽汁中膽汁酸及膽固醇含量的變化。結(jié)果：①隨著喂飼HCD時(shí)間增加，血漿TC、低密度脂蛋白膽固醇(LDL-C)及極低密度脂蛋白膽固醇(VLDL-C)顯著升高，3周組及4周組較對(duì)照組有顯著性差異(Ｐ＜0.05)，而高密度脂蛋白膽固醇(HDL-C)各組間雖無(wú)明顯差別(Ｐ＞0.05)，但其轉(zhuǎn)運(yùn)血漿膽固醇的作用明顯減弱(由對(duì)照組的80.00%降至4周組的3.68%)；②膽汁中膽固醇含量逐漸增加，3周組及4周組則明顯增高(Ｐ＜0.05)，而甘氨脫氧膽酸(GDCA)及甘氨膽酸(GCA)含量無(wú)明顯改變(Ｐ＞0.05)。本實(shí)驗(yàn)結(jié)果提示：血漿脂蛋白膽固醇所反映的脂蛋白代謝的變化，可能因影響體內(nèi)的膽固醇及膽汁酸等代謝而與膽囊結(jié)石的形成密切相關(guān)，并可能在結(jié)石的形成中起重要作用。

引用本文： 舒曄,趙紀(jì)春,張明儀,朱紅,程南生,肖路加. 血漿脂蛋白膽固醇水平與膽囊結(jié)石形成關(guān)系的實(shí)驗(yàn)研究. 中國(guó)普外基礎(chǔ)與臨床雜志, 1999, 6(2): 65-67. doi: 復(fù)制

1. 程南生， 付林，肖路加. 兔膽紅素鈣結(jié)石成石過(guò)程中血脂質(zhì)變化的研究. 華西醫(yī)科大學(xué)學(xué)報(bào)， 1994； 25(3)∶301.
2. 別平，孟憲均， 楊可禎等. 前列腺素環(huán)磷酸腺苷在膽囊結(jié)石形成中的作用. 中華外科雜志， 1987； 25(6)∶339.
3. Juniper K, Burson EN. Biliary tract studies Ⅱ, the significance of biliary crystals. Gastroenterology, 1957; 32(2)∶175.
4. 梅美珍主編. 動(dòng)脈粥樣硬化實(shí)驗(yàn)技術(shù). 湖南衡陽(yáng)： 中國(guó)病理生理學(xué)會(huì)動(dòng)脈粥樣硬化分會(huì)， 1989∶62.
5. Jorgensen T. Gallstones and plasma lipids in a Danish population. Scand J Gastroenterol, 1989; 24(8)∶916.
6. Howard B. Obesity cholelithiasis, and lipoprotein metabolism in man. Atheroscler Rev, 1986; 15(2)∶169.
7. Hayes KC, Khosla P, Kaiser A, et al. Dietary fat and cholesterol modulate the plasma lipoprotein distribution and production of pigment or cholesterol gallstones in hamsters. J Nutr, 1992; 122(2)∶374.
8. Whiting MJ, Wishart RA, Lewis G, et al. Bile acid synthesis by cultural rabbit hepatocytes: stimulation by three lipoprotein fractions. Biochim Biophys Acta, 1989; 1005(2)∶137.
9. Arbeeny CM, Rifici VA, Eder HA. The uptake of apoprotein and cholesterol ester of highdensity lipoproteins by perfused rat liver. Biochim Biophys Acta, 1987; 917(1)∶9.
10. Halloran LG, Schwartz CC, Bieber LL, et al. Evidence for high density lipoproteinfree cholesterol as the primary precursor for bileacid synthesis in man. Surgery, 1978; 84(1)∶1.
11. Scobey MW, Johnson FL, Rudel LL. Delivery of high density lipoprotein free and esterfied cholesterol to bile by perfused monkey liver. Am J Physiol, 1989; 257(4)∶G644.
12. Tao S, Tazuma S, Kajiyama G. Apolipoprotein A1 stabilizes phospholipid lamella and thus prolongs nucleation time in model bile system: an ultrastructural study. Biochim Biophys Acta, 1993; 1166(1)∶25.
  1. 1. 程南生， 付林，肖路加. 兔膽紅素鈣結(jié)石成石過(guò)程中血脂質(zhì)變化的研究. 華西醫(yī)科大學(xué)學(xué)報(bào)， 1994； 25(3)∶301.
  2. 2. 別平，孟憲均， 楊可禎等. 前列腺素環(huán)磷酸腺苷在膽囊結(jié)石形成中的作用. 中華外科雜志， 1987； 25(6)∶339.
  3. 3. Juniper K, Burson EN. Biliary tract studies Ⅱ, the significance of biliary crystals. Gastroenterology, 1957; 32(2)∶175.
  4. 4. 梅美珍主編. 動(dòng)脈粥樣硬化實(shí)驗(yàn)技術(shù). 湖南衡陽(yáng)： 中國(guó)病理生理學(xué)會(huì)動(dòng)脈粥樣硬化分會(huì)， 1989∶62.
  5. 5. Jorgensen T. Gallstones and plasma lipids in a Danish population. Scand J Gastroenterol, 1989; 24(8)∶916.
  6. 6. Howard B. Obesity cholelithiasis, and lipoprotein metabolism in man. Atheroscler Rev, 1986; 15(2)∶169.
  7. 7. Hayes KC, Khosla P, Kaiser A, et al. Dietary fat and cholesterol modulate the plasma lipoprotein distribution and production of pigment or cholesterol gallstones in hamsters. J Nutr, 1992; 122(2)∶374.
  8. 8. Whiting MJ, Wishart RA, Lewis G, et al. Bile acid synthesis by cultural rabbit hepatocytes: stimulation by three lipoprotein fractions. Biochim Biophys Acta, 1989; 1005(2)∶137.
  9. 9. Arbeeny CM, Rifici VA, Eder HA. The uptake of apoprotein and cholesterol ester of highdensity lipoproteins by perfused rat liver. Biochim Biophys Acta, 1987; 917(1)∶9.
  10. 10. Halloran LG, Schwartz CC, Bieber LL, et al. Evidence for high density lipoproteinfree cholesterol as the primary precursor for bileacid synthesis in man. Surgery, 1978; 84(1)∶1.
  11. 11. Scobey MW, Johnson FL, Rudel LL. Delivery of high density lipoprotein free and esterfied cholesterol to bile by perfused monkey liver. Am J Physiol, 1989; 257(4)∶G644.
  12. 12. Tao S, Tazuma S, Kajiyama G. Apolipoprotein A1 stabilizes phospholipid lamella and thus prolongs nucleation time in model bile system: an ultrastructural study. Biochim Biophys Acta, 1993; 1166(1)∶25.