• 1. 華西醫(yī)科大學(xué)附屬第一醫(yī)院普外科(成都610041)2. 華西醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院生化教研室;

為研究肝細(xì)胞低密度脂蛋白受體對(duì)膽汁中膽汁酸的影響及其在膽囊結(jié)石過(guò)程中可能的機(jī)理，采用高膽固醇膳食誘發(fā)兔膽囊結(jié)石模型，對(duì)進(jìn)食后1、2、3、4周組及對(duì)照組動(dòng)物，采用雙波長(zhǎng)薄層掃描法測(cè)定膽汁中膽汁酸，125I-LDL放免標(biāo)記法測(cè)定肝細(xì)胞低密度脂蛋白受體(LDLr)活性。結(jié)果： 高膽固醇膳食后2、3、4周組分別有4/10，6/10，7/10出現(xiàn)膽囊結(jié)石； 膽汁中甘氨膽酸明顯降低(Ｐ＜0.05)；125I-LDL與肝細(xì)胞低密度脂蛋白受體(LDLr)的最大結(jié)合力(Bmax)逐漸下降(Ｐ＜0.05)，解離常數(shù)(Kd)值逐漸升高(Ｐ＜0.05)。上述結(jié)果提示： 肝細(xì)胞低密度脂蛋白受體活性下降，可能導(dǎo)致膽汁中甘氨膽酸減少，致成石性膽汁形成。

引用本文： 趙紀(jì)春,舒曄,侯彩芳,程南生,肖路加,朱紅. 肝細(xì)胞低密度脂蛋白受體活性對(duì)膽汁中膽汁酸影響的實(shí)驗(yàn)研究. 中國(guó)普外基礎(chǔ)與臨床雜志, 1999, 6(4): 207-209. doi: 復(fù)制

1. 趙紀(jì)春， 肖路加， 朱紅等. 兔膽囊結(jié)石形成過(guò)程中脂代謝和載脂蛋白變化研究. 華西醫(yī)科大學(xué)學(xué)報(bào)， 1997； 28(4)∶361.
2. 孟高淵， 東云華， 李彬等. 九種混合膽汁酸的薄層光密度定量分析. 華西預(yù)防醫(yī)學(xué)， 1989； 43(1)∶24.
3. Makwell MA. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Ann Biochem, 1978; 87(2)∶206.
4. Brown MS, Goldstein JL. A receptormediated pathway for cholesterol homeostasis. Science, 1986; 232(1)∶34.
5. Seglen PO. Preparation of rat liver cells. Exp Cell Res, 1993; 82(2)∶391.
6. Kern F Jr. Effects of dietary cholesterol on cholesterol and bile acid homeostasis in patients with cholesterol gallstones. J Clin Invest, 1994; 93(3)∶1186.
7. Rudel L, Deckelman C, Wilson M, et al. Dietary cholesterol and down regulation of cholesterol 7αhydroxylase and cholesterol absorption in African green monkeys. J Clin Invest, 1994; 93(8)∶2463.
8. Khallon J, Riottot M, Parquet M, et al. Biodynamics of cholesterol and bile acids in the lithasic hamster. Br J Nutr, 1991; 66(3)∶479.
9. Hayes KC, Livingston A, Trautwein EA. Dietary impact on biliary lipids and gallstones. Ann. Rev Nutr, 1992; 12∶299.
10. Nicolosi RJ, Stucchi AF, Kowala MC, et al. Effect of dietary fat saturation and cholesterol on LDL composition and metabolism. Arteriosclerosis, 1990; 10(1)∶119.
11. Everson GT, Daggy BP, Mckinley GT, et al. Dietary fiber (psyllium) lowers LDL cholesterol by stimulating bile acid synthesis in man. Hepatology, 1991; 14(Suppl)∶148a.
12. Reihner E, Angelin B, Rudling M, et al. Regulation of hepatic cholesterol metabolism in human: stimulatory effects of cholestyramine on HMGCoA reductase activity and low density lipoprotein receptor expression in gallstone patients. J Lipid Res, 1990; 31(12)∶2219.
13. Nanjee MN, Miller NE. Human hepatic low density lipoprotein receptors : associations of receptor activities in vitro with plasma lipid and apolipoprotein concentrations in vivo. Biochem Biophys Acta, 1989; 1002(2)∶245.
  1. 1. 趙紀(jì)春， 肖路加， 朱紅等. 兔膽囊結(jié)石形成過(guò)程中脂代謝和載脂蛋白變化研究. 華西醫(yī)科大學(xué)學(xué)報(bào)， 1997； 28(4)∶361.
  2. 2. 孟高淵， 東云華， 李彬等. 九種混合膽汁酸的薄層光密度定量分析. 華西預(yù)防醫(yī)學(xué)， 1989； 43(1)∶24.
  3. 3. Makwell MA. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Ann Biochem, 1978; 87(2)∶206.
  4. 4. Brown MS, Goldstein JL. A receptormediated pathway for cholesterol homeostasis. Science, 1986; 232(1)∶34.
  5. 5. Seglen PO. Preparation of rat liver cells. Exp Cell Res, 1993; 82(2)∶391.
  6. 6. Kern F Jr. Effects of dietary cholesterol on cholesterol and bile acid homeostasis in patients with cholesterol gallstones. J Clin Invest, 1994; 93(3)∶1186.
  7. 7. Rudel L, Deckelman C, Wilson M, et al. Dietary cholesterol and down regulation of cholesterol 7αhydroxylase and cholesterol absorption in African green monkeys. J Clin Invest, 1994; 93(8)∶2463.
  8. 8. Khallon J, Riottot M, Parquet M, et al. Biodynamics of cholesterol and bile acids in the lithasic hamster. Br J Nutr, 1991; 66(3)∶479.
  9. 9. Hayes KC, Livingston A, Trautwein EA. Dietary impact on biliary lipids and gallstones. Ann. Rev Nutr, 1992; 12∶299.
  10. 10. Nicolosi RJ, Stucchi AF, Kowala MC, et al. Effect of dietary fat saturation and cholesterol on LDL composition and metabolism. Arteriosclerosis, 1990; 10(1)∶119.
  11. 11. Everson GT, Daggy BP, Mckinley GT, et al. Dietary fiber (psyllium) lowers LDL cholesterol by stimulating bile acid synthesis in man. Hepatology, 1991; 14(Suppl)∶148a.
  12. 12. Reihner E, Angelin B, Rudling M, et al. Regulation of hepatic cholesterol metabolism in human: stimulatory effects of cholestyramine on HMGCoA reductase activity and low density lipoprotein receptor expression in gallstone patients. J Lipid Res, 1990; 31(12)∶2219.
  13. 13. Nanjee MN, Miller NE. Human hepatic low density lipoprotein receptors : associations of receptor activities in vitro with plasma lipid and apolipoprotein concentrations in vivo. Biochem Biophys Acta, 1989; 1002(2)∶245.