• 1. 解放軍第97醫(yī)院普外科(徐州221004).2. 南京軍區(qū)南京總醫(yī)院全軍普外研究所;

為了解急性壞死性胰腺炎(acute necrotizing pancreatitis，ANP)時血漿內(nèi)皮素(endothelin，ET)的變化及其病理意義，進行了前瞻性動物實驗。即將SD大鼠隨機分為3組： 急性壞死性胰腺炎組(ANP組，n＝24)，經(jīng)膽胰管注入5%牛磺膽酸鈉(STC，1 ml/kg)制造ANP模型； 假手術組(SO組，n＝24)和血小板激活因子拮抗劑BN50739組(BN組，n＝24)。測定血漿ET及血小板激活因子(platelet activating factor，PAF)水平和胰腺微循環(huán)血流量，并進行胰腺組織病理學評分。結(jié)果： ANP組在誘發(fā)胰腺炎后胰腺微循環(huán)血流量立即大幅度下降，于3小時時有所回升，以后再持續(xù)下降。血漿ET、PAF水平及病理記分均顯著高于SO組。BN組血流顯著改善，血漿ET、PAF水平及病理記分顯著低于ANP組。結(jié)論： 在STC誘發(fā)的大鼠ANP發(fā)病之初，存在胰腺缺血再灌注(I/R)現(xiàn)象，導致內(nèi)皮細胞損傷，血漿ET及PAF增加。I/R損傷、PAF與ET互相作用，形成惡性循環(huán)，加重胰腺病變。

引用本文： 李璽,黎介壽,朱維銘,李寧. 急性壞死性胰腺炎大鼠血漿內(nèi)皮素的改變及其意義. 中國普外基礎與臨床雜志, 1999, 6(6): 331-333. doi: 復制

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1. Takakuwa T, Endo S, Nakae H, et al. Relationships between plasma levels of typeI phospholipase A2, PAFacetylhydrolase, leukotriene B4, complements, endothelin1, and thrombomodulin in patients with sepsis. Res Commun Chem Pathol Pharmacol, 1994; 84(3)∶271.
2. Bassi D, Kollias N, FernandezDel Castillo C, et al. Impairment of pancreatic microcirculation correlates with the sererity of acute experimental pancreatitis. J Am Coll Surg, 1994; 179(3)∶257.
3. McMillen MA, Sumpio BE. Endothelins: polyfunctional cytokines. J Am Coll Surg, 1995; 180(5)∶621.
4. Aho HJ, Koskensalo SML, Nevalainen TJ. Experimental pancreatitis in the rat. Sodium taurocholateinduced acute haemorrhagic pancreatitis. Scand J Gastroenterol, 1980; 15(4)∶411.
5. Pinckard RN, Farr RS, Hanahan DJ. Physicochemical and functional identity of rabbit plateletactivating factor(PAF) released in vivo during IgE anaphylaxis with PAF released in vitro from IgE sensitized basophils. J Immunol, 1979; 123(4)∶1847.
6. Schmidt J, Lewandrowski K, FernanderDel Castillo C, et al. Histopathologic correlates of serum amylase activity in acute experimental pancreatitis. Dig Dis Sci, 1992; 37(9)∶1426.
7. Kusterer K, Poschmann T, Friedemann A, et al. Arterial constriction, ischemiareperfusion, and leukocyte adherence in acute pancreatitis. Am J Physiol, 1993; 265(1Pt1)∶G165.
8. Hoffmann TF, Leiderer R, Waldner H, et al. Ischemia reperfusion of the pancrease: a new in vivo model of acute pancreatitis in rats. Res Exp Med Berl, 1995; 195(3)∶125.
9. Anderson BO, Bensard DD, Harken AH. The role of platelet activating factor and its antagonists in shock, sepsis and multiple organ failure. Surg Gynecol Obstet, 1991; 1972(5)∶415.
10. Mustafa SB, Gandht CR, Harvey SAK, et al. Endothelin stimulates plateletactivating factor synthesis by cultured rat kupffer cells. Hepatology, 1995; 21(2)∶545.
  1. 1. Takakuwa T, Endo S, Nakae H, et al. Relationships between plasma levels of typeI phospholipase A2, PAFacetylhydrolase, leukotriene B4, complements, endothelin1, and thrombomodulin in patients with sepsis. Res Commun Chem Pathol Pharmacol, 1994; 84(3)∶271.
  2. 2. Bassi D, Kollias N, FernandezDel Castillo C, et al. Impairment of pancreatic microcirculation correlates with the sererity of acute experimental pancreatitis. J Am Coll Surg, 1994; 179(3)∶257.
  3. 3. McMillen MA, Sumpio BE. Endothelins: polyfunctional cytokines. J Am Coll Surg, 1995; 180(5)∶621.
  4. 4. Aho HJ, Koskensalo SML, Nevalainen TJ. Experimental pancreatitis in the rat. Sodium taurocholateinduced acute haemorrhagic pancreatitis. Scand J Gastroenterol, 1980; 15(4)∶411.
  5. 5. Pinckard RN, Farr RS, Hanahan DJ. Physicochemical and functional identity of rabbit plateletactivating factor(PAF) released in vivo during IgE anaphylaxis with PAF released in vitro from IgE sensitized basophils. J Immunol, 1979; 123(4)∶1847.
  6. 6. Schmidt J, Lewandrowski K, FernanderDel Castillo C, et al. Histopathologic correlates of serum amylase activity in acute experimental pancreatitis. Dig Dis Sci, 1992; 37(9)∶1426.
  7. 7. Kusterer K, Poschmann T, Friedemann A, et al. Arterial constriction, ischemiareperfusion, and leukocyte adherence in acute pancreatitis. Am J Physiol, 1993; 265(1Pt1)∶G165.
  8. 8. Hoffmann TF, Leiderer R, Waldner H, et al. Ischemia reperfusion of the pancrease: a new in vivo model of acute pancreatitis in rats. Res Exp Med Berl, 1995; 195(3)∶125.
  9. 9. Anderson BO, Bensard DD, Harken AH. The role of platelet activating factor and its antagonists in shock, sepsis and multiple organ failure. Surg Gynecol Obstet, 1991; 1972(5)∶415.
  10. 10. Mustafa SB, Gandht CR, Harvey SAK, et al. Endothelin stimulates plateletactivating factor synthesis by cultured rat kupffer cells. Hepatology, 1995; 21(2)∶545.