• 1. 廣州軍區(qū)157中心醫(yī)院普外科(廣州510510)2. 第三軍醫(yī)大學第三附屬醫(yī)院普外科;

用MTT比色分析法和流式細胞術在體外研究了5肽胃泌素(pentagastrin,PG)及胃泌素受體拮抗劑丙谷胺(proglumide, PGL)對人大腸癌細胞株SW480的作用。結果顯示：PG能促進SW480細胞生長；PGL單用時對SW480細胞沒有影響，當PG與PGL合用時，PGL呈劑量依賴地拮抗PG的作用，PGL在32μg/ml以上時，拮抗作用趨于恒定。提示胃泌素的作用是通過其受體發(fā)揮的。PG促進SW480細胞合成DNA、蛋白質，促進SW480細胞從G0/G1期過度到S期和G2M期；PGL完全拮抗這種作用，提示胃泌素的作用在受體后是通過影響細胞周期實現的。

引用本文： 謝尚奎,何雙梧,吳印愛,陳明珠,羅真東. 胃泌素及其受體拮抗劑對人大腸癌細胞株SW480作用的研究. 中國普外基礎與臨床雜志, 1998, 5(1): 1-3. doi: 復制

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  1. 1. Johnson LR. New aspects of the trophic actions of gastrointestinal hormones. Gastroenterology, 1977; 72(3)∶788.
  2. 2. Watson S, Durrant LG, Morris D. Gastrin:growth enhancing effects on human gastric and colonic tumor cells. Br J Cancer, 1989; 59(3)∶554.
  3. 3. Mosman T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods, 1983; 63(1)∶55.
  4. 4. 宋平根，李素文.流式細胞術的原理和應用.第1版.北京:北京師范大學出版社， 1992∶254～268.
  5. 5. Kusyk CJ, Mcniel NO, Johnson LR, et al. Stimulation of growth of a colon cancer cell line by gastrin. Am J Physiol, 1986; 251(7)∶G597.
  6. 6. Imdah LA, Eggstein S, Crone C, et al. Growth of colorectal carcinoma cells: regulation in vitro by gastrin, pentagastrin and the gastrinreceptor antagonist proglumide. J Cancer Clin Oncol, 1989; 115(4)∶388.
  7. 7. Smith JP, Solomon TE. Effects of gastrin, proglumide, and somatostatin on growth of human colon cancer. Gastroenterology,1988; 95(1)∶1541.
  8. 8. CivitellS, GalganiP, PacchiarottiMC, et al. Blood gastrin and colorectal neoplasma. GChir, 1994; 15(5)∶219.
  9. 9. De-Bruine AP, Wiggers T, Beek C, et al. Endocrine cells in colorectal adenocarcinomas: in cidence,hormone profile and prognostic relevance. In J Cancer, 1993; 54(5)∶765.