應(yīng)用流式細(xì)胞術(shù)和細(xì)胞免疫熒光染色技術(shù)，對46例大腸腫瘤細(xì)胞ras p21表達(dá)和DNA含量進(jìn)行了定量分析。結(jié)果：大腸癌的ras p21表達(dá)陽性率為65.7%，DNA異倍體率為74.3%，大腸癌細(xì)胞ras p21表達(dá)量明顯高于腺瘤和正常粘膜；DNA倍體和細(xì)胞增殖指數(shù)值與ras p21表達(dá)量密切相關(guān)。本實(shí)驗(yàn)結(jié)果提示：測定大腸癌的ras p21表達(dá)和DNA含量將有助于其預(yù)后判斷。
引用本文： 徐亮,盧光宇. 大腸癌ras p21表達(dá)和DNA含量定量分析. 中國普外基礎(chǔ)與臨床雜志, 1998, 5(1): 4-6. doi: 復(fù)制
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| 6. | Miyahara M, Saito T, Kaketani K, et al. Clinical significance of ras p21 overexpression for patients with an advanced colorectal cancer. Dis Colon Rectum, 1991; 34∶1097. |
| 7. | Jansson DS. An immunohistochemical analysis of ras oncogene expression in epithelial neoplasms of the colon. Cancer, 1990; 65∶1329. |
| 8. | Wang Q. Biopathologic characteristics of DNA content in crypt cells of transitional mucosa adjacent to carcinoma of the rectum and rectosigmoid. Dis Colon Rectum, 1992; 25∶670. |
| 9. | Merkel DE， Mcguire WL. Ploidy proliferative activity and prognosis， DNA flow cytometry of solid tumors. Cancer, 1990; 65∶1194. |
| 10. | Barbara F, Chacho MS, Rosemen DL, et al. Multiparameter flow cytometric analysis of colon polyps. Am J Clin Pathol,1987; 87∶313. |
| 11. | Quirke P， Fozard JBJ, Dixon MF, et al. DNA aneuploidy in human colon carcinomas. Br J Cancer, 1986; 53∶477. |
| 12. | Sun XF. Ras p21 expression in relation to DNA ploidy, Sphase frac-tion and prognosis in colorectal adenocarcinomas.Cytometry, 1991; Suppl 5∶59. |
| 13. | Michelasis F, Erroi F, Roncella M, et al. Ras oncogene and the acquisition of metastasizing properties by rectal adenocarcinoma. Dis Colon Rectum, 1989; 32∶665. |
- 1. Hedley DM, Yonemura Y, Suzuki H, et al. Method for analysis of cellular DNA content of paraffin embedded pathological material using flow cytometry. J Histochem Cytochem, 1983; 31∶33.
- 2. Morkve O. Flow cytometric measurement of p53 protein expression and DNA content in paraffin embedded tissue from brochial carcinomas. Cytometry, 1991; 12∶438.
- 3. Barbacid M, Saito H, Clement JM, et al. Ras genes. Ann Rev Biochem,1987; 56∶779.
- 4. Bos JL. Ras oncogene in human cancer. Cancer Res, 1989; 49∶4682.
- 5. Scott N，Quirke P. Molecular biology of colorectal neoplasia. Gut, 1993; 34(3)∶289.
- 6. Miyahara M, Saito T, Kaketani K, et al. Clinical significance of ras p21 overexpression for patients with an advanced colorectal cancer. Dis Colon Rectum, 1991; 34∶1097.
- 7. Jansson DS. An immunohistochemical analysis of ras oncogene expression in epithelial neoplasms of the colon. Cancer, 1990; 65∶1329.
- 8. Wang Q. Biopathologic characteristics of DNA content in crypt cells of transitional mucosa adjacent to carcinoma of the rectum and rectosigmoid. Dis Colon Rectum, 1992; 25∶670.
- 9. Merkel DE， Mcguire WL. Ploidy proliferative activity and prognosis， DNA flow cytometry of solid tumors. Cancer, 1990; 65∶1194.
- 10. Barbara F, Chacho MS, Rosemen DL, et al. Multiparameter flow cytometric analysis of colon polyps. Am J Clin Pathol,1987; 87∶313.
- 11. Quirke P， Fozard JBJ, Dixon MF, et al. DNA aneuploidy in human colon carcinomas. Br J Cancer, 1986; 53∶477.
- 12. Sun XF. Ras p21 expression in relation to DNA ploidy, Sphase frac-tion and prognosis in colorectal adenocarcinomas.Cytometry, 1991; Suppl 5∶59.
- 13. Michelasis F, Erroi F, Roncella M, et al. Ras oncogene and the acquisition of metastasizing properties by rectal adenocarcinoma. Dis Colon Rectum, 1989; 32∶665.