• 1. 研究生，現(xiàn)在蘭州醫(yī)學(xué)院第二附屬醫(yī)院外科(蘭州730030)2. 北京醫(yī)科大學(xué)人民醫(yī)院;

應(yīng)用3H-哌唑嗪結(jié)合分析法測(cè)定人正常肝組織膜α1-腎上腺素受體濃度，以了解影響其受體結(jié)合的實(shí)驗(yàn)條件。結(jié)果：在膜蛋白濃度約為1.5mg/ml、3H-哌唑嗪濃度為0.1～2.0 nmol/L、pH 7.4、37℃反應(yīng)20分鐘的適宜條件下，8例正常肝組織膜α1-腎上腺素受體與配基結(jié)合均呈飽和狀態(tài)，受體的最大結(jié)合容量(Bmax)、平衡解離常數(shù)(Kd)、組織受體最大結(jié)合容量(RMC)和Hill系數(shù)分別為142.1±14.1 fmol/mg蛋白、0.2382±0.0548 nM、739.0±167.6 fmol/g肝和0.90±0.03。本實(shí)驗(yàn)結(jié)果提示：應(yīng)用3H-哌唑嗪結(jié)合分析法測(cè)定人肝組織膜α1受體時(shí)實(shí)驗(yàn)條件十分重要，在肝臟病理生理研究中應(yīng)充分考慮。

引用本文： 張有成,冷希圣,彭吉潤(rùn). 放射配基結(jié)合法測(cè)定人肝組織膜α1-腎上腺素受體的條件選擇. 中國(guó)普外基礎(chǔ)與臨床雜志, 1998, 5(4): 202-204. doi: 復(fù)制

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  2. 2. 冷希圣，黃萃庭，杜如昱.組織胺、去甲腎上腺素、多巴胺、胰高血糖素對(duì)肝臟血流動(dòng)力學(xué)的影響.北京醫(yī)學(xué)院學(xué)報(bào)， 1982； 14(1)∶1.
  3. 3. Michalopoulos G, Cianciulli HD, Novotny AR, et al. Liver regeneration studies with rat hepatocytes in primary culture. Cancer Res, 1982; 42(8)∶4673.
  4. 4. Williams LT, Mullikin D, Lefkowitz RJ. Identification of αadrenergic receptors in uterine smooth muscle membranes by 3Hdihydroergocryptine binding. J Biol Chem, 1976; 251(22)∶6915.
  5. 5. Bradford MM. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of proteindye binding. Anal Biochem, 1976; 72(2)∶248.
  6. 6. 夏宗勤主編.實(shí)驗(yàn)核醫(yī)學(xué)與核藥學(xué).第1版.上海：同濟(jì)大學(xué)出版社， 1989∶220～240.
  7. 7. 馮國(guó)平.受體分析常用數(shù)據(jù)處理方法的比較.中華核醫(yī)學(xué)雜志，1987; 7(3)∶167.
  8. 8. Kawai Y, Powell A, Arinze IJ. Adrenergic receptors in human liver plasma membranes: predominance of beta 2and alpha 1receptor subtypes. J Clin Endocrinol Metab, 1986; 62(5)∶827.
  9. 9. Hwang TL, Lau YT, Huang SF,et al. Changes of α1adrenergic receptors in human liver during intraabdominal sepsis. Hepatology, 1994; 20(3)∶638.