• 華西醫(yī)科大學(xué)附屬第一醫(yī)院外科(成都610041);

為減損排斥反應(yīng)性淋巴細(xì)胞克隆，采用異型雙功能試劑SPDP、2-IT連接antiCD4、CD8單克隆抗體與天花粉毒素(TCS)，應(yīng)用Sephacryl S-200分離游離TCS，采用SDS-PAGE電泳及細(xì)胞毒性分析測(cè)定合成的免疫毒素的純度和生物學(xué)活性。電泳結(jié)果顯示，連接混合物中含有連接的免疫毒素、游離的單克隆抗體和TCS，Sephacryl S-200可除去游離的TCS。體外，免疫毒素可特異性殺傷大鼠脾細(xì)胞，其作用具有劑量依賴性。由此提示抗CD4、CD8免疫毒素可以誘導(dǎo)免疫無反應(yīng)性。

引用本文： 蘭平,劉嘉林,嚴(yán)律南,肖路加. 免疫毒素的制備及特異性淋巴細(xì)胞無反應(yīng)性的誘導(dǎo). 中國(guó)普外基礎(chǔ)與臨床雜志, 1998, 5(6): 321-323. doi: 復(fù)制

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2. Cobbold SP, Adams E, Marshall SE, et al. Mechanisms of peripheral tolerance and suppression induced by monoclonal antibodies to CD4 and CD8. Immunol Rev, 1996; 149∶5.
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4. Wang QC. TrichosanthinmAb conjugate sepcifically cytotoxic to human hepatoma cell in vitro. Cancer Res, 1991; 51(12)∶3353.
5. Ellman GL. Tissue sulfhgdryl groups. Arch Biochem Biophy, 1959; 82(1)∶70.
6. Kagi D, Vignaux F, Ledermann B, et al. Fas and perforin pathways as major mechanisms of T cellmediated cytotoxicity. Science, 1994; 265(5171)∶528.
7. Stalder T, Hahn S, Erb P. Fas antigen is a major targed molecule for CD4＋T cell mediated cytotoxicity. J Immunol, 1994; 152(3)∶1127.
8. Smith CV, Sablinski T, Arn JS, et al. In vivo treatment with monoclonal antibodies directed against CD4 and CD8 antigens in miniature swine. J Immunother Emphasis Tumor Immunol, 1994; 16(2)∶105.
9. Kochi SH, Collier RJ. DNA fragmentation and cytolysis in V937 cell treatment with diphtheria toxin or other inhibitors of protein synthesis. Exp Cell Res, 1993; 208(1)∶296.
10. Waddick KG, Myers DE, Gunther R, et al. In vivo antileukemic activity of B43pokweed antiviral protein against radiation resistant human Bcell procursor leukemia cells. Blood, 1996; 186(11)∶4228.
  1. 1. Brennan DC, Mohanakumar T, Flye MW. Donorspicific transfusion and donor bone marrow infusion in renal transplantation tolerance, a review of effecacy and mechanisms. Am J Kind Dis, 1995; 26(5)∶701.
  2. 2. Cobbold SP, Adams E, Marshall SE, et al. Mechanisms of peripheral tolerance and suppression induced by monoclonal antibodies to CD4 and CD8. Immunol Rev, 1996; 149∶5.
  3. 3. Carlsson J, Drevin H, Axen R. Protein thilolation and raversible proteinprotein conjugation. Nsuxxinimidy13(2pyridyldithio) propionate, a new heterobifunctional reagent. Biochem J, 1978; 173(3)∶723.
  4. 4. Wang QC. TrichosanthinmAb conjugate sepcifically cytotoxic to human hepatoma cell in vitro. Cancer Res, 1991; 51(12)∶3353.
  5. 5. Ellman GL. Tissue sulfhgdryl groups. Arch Biochem Biophy, 1959; 82(1)∶70.
  6. 6. Kagi D, Vignaux F, Ledermann B, et al. Fas and perforin pathways as major mechanisms of T cellmediated cytotoxicity. Science, 1994; 265(5171)∶528.
  7. 7. Stalder T, Hahn S, Erb P. Fas antigen is a major targed molecule for CD4＋T cell mediated cytotoxicity. J Immunol, 1994; 152(3)∶1127.
  8. 8. Smith CV, Sablinski T, Arn JS, et al. In vivo treatment with monoclonal antibodies directed against CD4 and CD8 antigens in miniature swine. J Immunother Emphasis Tumor Immunol, 1994; 16(2)∶105.
  9. 9. Kochi SH, Collier RJ. DNA fragmentation and cytolysis in V937 cell treatment with diphtheria toxin or other inhibitors of protein synthesis. Exp Cell Res, 1993; 208(1)∶296.
  10. 10. Waddick KG, Myers DE, Gunther R, et al. In vivo antileukemic activity of B43pokweed antiviral protein against radiation resistant human Bcell procursor leukemia cells. Blood, 1996; 186(11)∶4228.