溫?zé)崧?lián)合化療、放療及免疫治療有協(xié)同抗癌作用，并能減輕對(duì)正常組織的副作用。本實(shí)驗(yàn)選擇絲裂霉素C(MMC)及5-氟脲嘧啶(5-Fu)聯(lián)合溫?zé)狍w外作用于人胃癌細(xì)胞株(MGC-803)
，檢測(cè)其作用后活癌細(xì)胞百分計(jì)數(shù)、克隆形成率及存活分?jǐn)?shù)，以觀察溫?zé)崧?lián)合MMC或5-Fu的細(xì)胞毒作用。結(jié)果表明：溫?zé)崧?lián)合MMC有協(xié)同抗癌作用，且隨溫度的升高其作用逐漸增強(qiáng)，
與單純加溫組比較有顯著性差異(P lt;0.01)；而溫?zé)崧?lián)合5-Fu無(wú)協(xié)同抗癌作用，但與單純加溫組比較，在較低溫度(37℃)時(shí)有顯著性差異(P lt;0.01)。本實(shí)驗(yàn)結(jié)果為臨床應(yīng)用提供了依據(jù)。
引用本文： 王亞旭,王代科,劉寶華. 溫?zé)崧?lián)合絲裂霉素、5-氟脲嘧啶對(duì)人胃癌細(xì)胞的體外效應(yīng). 中國(guó)普外基礎(chǔ)與臨床雜志, 1997, 4(1): 1-3. doi: 復(fù)制
| 1. | Love R.Effent of hyperhtermia on normal and neoplastic cells in vitro.Cancer Res,1970;30 : 1525. |
| 2. | Mondovi B. The biochemical mechanism of selective heat sensitivity of cancer cells II . Studies on nucleic acids and protein synthesis. Eur J Cancer,1969;. |
| 3. | 松田忠義. 溫?zé)峄瘜W(xué)療法的現(xiàn)狀. 日本醫(yī)學(xué)介紹,1989;10(11) : 496. |
| 4. | 王凱華. 人體低分化粘液腺癌細(xì)胞系MGC-803的建立及其生物學(xué)特性的初步觀察. 實(shí)驗(yàn)生物學(xué)學(xué)報(bào), 1983;16(3) : 257. |
| 5. | : 137. |
| 6. | Stehlin JS. Results of hyperthermic perfusion for melsnoma of the extremities. Surg Gynecol Obster, 1975;140:338. |
| 7. | Norbert JR.Shintsu Lu,Solm M. Hyperthermia and human leukocyte functions:DNA,RNA,and total protein systhesis after exposure to <41℃ or >42.5℃ hyperthermia.Cancer Res,1985;45 : 3076. |
| 8. | 趙曉光,張鳳蘭,徐浩梁等. 增強(qiáng)高溫對(duì)腫瘤效應(yīng)一些因素的探討. 中華病理學(xué)雜志,1988;17(1) : 69. |
| 9. | Storm FK. Hyperthermia in cancer therapy.G.K Hall Medical Publishers.Boston:Massachusetts,1983 : 142. |
| 10. | 南京醫(yī)學(xué)院,南京醫(yī)學(xué)院附一院.臨床藥物手冊(cè).第2版.上海:上?？茖W(xué)技術(shù)出版社,1986 : 86. |
| 11. | Beverly AT,Charles DK,Katherine AK,et al. Enhancement by hyperthermia of the in vitro cytotoxity of mitomycin C toward hypoxic tumor cells. Cancer Res,1979;39 : 2245. |
| 12. | Storm FK,William HH,Robert SE,et al. Normal tissue and solid tumor effects of hyperthermia in animal models and clinical trials.Cancer Res,1979;39 : 2245. |
| 13. | 楊耀琴,楊虎川,陶惠紅等.溫?zé)?39~45℃)合并絲裂霉素C對(duì)BGC-823人胃癌細(xì)胞的抑制效應(yīng).上海鐵道醫(yī)學(xué)院學(xué)報(bào),1990;4(2) : 92. |
| 14. | Yasunori E,Shunji K,Motofumi Y,et al. Hyperthermia enhances the i9nhibition of tumor growth by 1-(2-tetrahydrofuryl)-5-Fluorouracil/Uracil(1:4)in tumorsin mice and humans.Cancer,1992;70 : 1177. |
| 15. | 寧守誠(chéng),張錫澤.惡性腫瘤加熱治療的進(jìn)展.中國(guó)腫瘤臨床,1986;13(4) : 220. |
- 1. Love R.Effent of hyperhtermia on normal and neoplastic cells in vitro.Cancer Res,1970;30 : 1525.
- 2. Mondovi B. The biochemical mechanism of selective heat sensitivity of cancer cells II . Studies on nucleic acids and protein synthesis. Eur J Cancer,1969;.
- 3. 松田忠義. 溫?zé)峄瘜W(xué)療法的現(xiàn)狀. 日本醫(yī)學(xué)介紹,1989;10(11) : 496.
- 4. 王凱華. 人體低分化粘液腺癌細(xì)胞系MGC-803的建立及其生物學(xué)特性的初步觀察. 實(shí)驗(yàn)生物學(xué)學(xué)報(bào), 1983;16(3) : 257.
- 5. : 137.
- 6. Stehlin JS. Results of hyperthermic perfusion for melsnoma of the extremities. Surg Gynecol Obster, 1975;140:338.
- 7. Norbert JR.Shintsu Lu,Solm M. Hyperthermia and human leukocyte functions:DNA,RNA,and total protein systhesis after exposure to <41℃ or >42.5℃ hyperthermia.Cancer Res,1985;45 : 3076.
- 8. 趙曉光,張鳳蘭,徐浩梁等. 增強(qiáng)高溫對(duì)腫瘤效應(yīng)一些因素的探討. 中華病理學(xué)雜志,1988;17(1) : 69.
- 9. Storm FK. Hyperthermia in cancer therapy.G.K Hall Medical Publishers.Boston:Massachusetts,1983 : 142.
- 10. 南京醫(yī)學(xué)院,南京醫(yī)學(xué)院附一院.臨床藥物手冊(cè).第2版.上海:上?？茖W(xué)技術(shù)出版社,1986 : 86.
- 11. Beverly AT,Charles DK,Katherine AK,et al. Enhancement by hyperthermia of the in vitro cytotoxity of mitomycin C toward hypoxic tumor cells. Cancer Res,1979;39 : 2245.
- 12. Storm FK,William HH,Robert SE,et al. Normal tissue and solid tumor effects of hyperthermia in animal models and clinical trials.Cancer Res,1979;39 : 2245.
- 13. 楊耀琴,楊虎川,陶惠紅等.溫?zé)?39~45℃)合并絲裂霉素C對(duì)BGC-823人胃癌細(xì)胞的抑制效應(yīng).上海鐵道醫(yī)學(xué)院學(xué)報(bào),1990;4(2) : 92.
- 14. Yasunori E,Shunji K,Motofumi Y,et al. Hyperthermia enhances the i9nhibition of tumor growth by 1-(2-tetrahydrofuryl)-5-Fluorouracil/Uracil(1:4)in tumorsin mice and humans.Cancer,1992;70 : 1177.
- 15. 寧守誠(chéng),張錫澤.惡性腫瘤加熱治療的進(jìn)展.中國(guó)腫瘤臨床,1986;13(4) : 220.