目的 探討銅綠假單胞菌(PA)的密度感應(yīng)系統(tǒng)(Qs)在氣道黏液高分泌中的作用。方法 60只大鼠隨機(jī)分為3組(野生型菌株P(guān)AO1組、QS基因突變型菌株P(guān)AO1-JP2組和對(duì)照組)，分別將包被不同亞型PA的硅膠管置人大鼠一側(cè)主支氣管，建立慢性肺部感染模型，對(duì)照組則用生理鹽水浸泡硅膠管。28 d后處死大鼠，取肺組織和支氣管肺泡灌洗液(BALF)采用AB．PAS染色、ELISA和RT-PCR等方法檢測(cè)氣道內(nèi)黏蛋白MUC5AC蛋白及mRNA的表達(dá)水平，比較野生型菌株P(guān)AO1和Qs突變型菌株P(guān)AO1-JP2對(duì)大鼠氣道黏液分泌的影響。結(jié)果 PAO1組較PAO1-JP2組氣道黏膜上皮的杯狀細(xì)胞數(shù)目顯著增多(P lt;0．05)，對(duì)照組的杯狀細(xì)胞數(shù)目極少。PAO1組MUC5ACmRNA表達(dá)量較PAO1-JP2組顯著增多(P lt;0．05)，對(duì)照組MUC5AC mRNA僅有少量表達(dá)。PAO1組BALF中MUC5AC蛋白分泌量明顯高于PAO1-JP2組(P lt;0．05)。結(jié)論 PA的QS重要基因缺損導(dǎo)致氣道黏液分泌的能力顯著下降。
引用本文： 張?zhí)O,楊青梅,沈策. 銅綠假單胞菌密度感應(yīng)系統(tǒng)對(duì)氣道黏液高分泌的影響. 中國(guó)呼吸與危重監(jiān)護(hù)雜志, 2008, 08(2): 93-96. doi: 復(fù)制
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