目的 探討不可分型流感嗜血桿菌（NTHi）作用于呼吸道上皮細(xì)胞株（A549），表達(dá)前炎癥細(xì)胞因子的變化及其干預(yù)措施。方法 用NTHi、NTHi+紅霉素（10 mg/L）、NTHi+慶大霉素（100 mg/L）、NTHi+地塞米松（100 μmol/L）分別感染A549細(xì)胞，用核因子κB（NF-κB）抑制劑二硫氨基甲酸吡啶（PDTC，200 μmol/L）預(yù)處理NTHi感染的A549細(xì)胞，24 h后觀察細(xì)胞的形態(tài)學(xué)改變，并檢測(cè)上清液中白細(xì)胞介素8（IL-8）、腫瘤壞死因子α（TNF-α）的水平和細(xì)胞間黏附分子1（ICAM-1）的表達(dá)。結(jié)果 NTHi作用A549細(xì)胞24 h后，引起部分上皮細(xì)胞變形、死亡。NTHi能顯著誘導(dǎo)A549細(xì)胞株分泌IL-8、表達(dá)ICAM-1，紅霉素能夠抑制其誘導(dǎo)作用；慶大霉素具有殺滅NTHi作用，但不能抑制IL-8分泌和ICAM-1表達(dá)；地塞米松能抑制A549分泌IL-8，降低ICAM-1的表達(dá)，但不能殺滅NTHi。PDTC（200 μmol/L）能阻斷A549細(xì)胞在NTHi作用后IL-8的高表達(dá)。TNF-α在各組培養(yǎng)上清中均不能檢測(cè)到。結(jié)論 NTHi顯著誘導(dǎo)人肺上皮細(xì)胞系表達(dá)前炎癥因子，該作用可能通過(guò)NF-κB通路。紅霉素在殺滅NTHi的同時(shí)還具有抗炎作用。
引用本文： 修清玉,錢(qián)建美,王桂芳,黃海,方正. 不可分型流感嗜血桿菌誘導(dǎo)A549細(xì)胞分泌和表達(dá)前炎癥細(xì)胞因子及機(jī)制研究. 中國(guó)呼吸與危重監(jiān)護(hù)雜志, 2008, 08(3): 210-213. doi: 復(fù)制
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- 1. 1 方正，修清玉，羅文侗. 慢性阻塞性肺疾病中的流感嗜血桿菌感染. 第二軍醫(yī)大學(xué)學(xué)報(bào) 1999, 20:624-626.
- 2. 4 錢(qián)建美，修清玉，王桂芳,等.不可分型流感嗜血桿菌對(duì)氣管黏膜上皮細(xì)胞的作用.第二軍醫(yī)大學(xué)學(xué)報(bào),2004，25：769-772.
- 3. Sethi S, Evans N, Grant BJ, et al. New strains of bacteria and exacerbations of chronic obstructive pulmonary disease. N Engl J Med, 2002, 347:465-471.
- 4. Murphy TF, Brauer AL, Schiffmacher AT,et al.Persistent colonization by Haemophilus influenzae in chronic obstructive pulmonary disease. Am J Respir Crit Care Med, 2004,170:266–272.
- 5. Khair OA, Devalia JL, Abdelaziz MM, et al. Effect of Haemophilus influenzae endotoxin on the synthesis of IL-6,IL-8,TNF-α and expression of ICAM-1 in cultured human bronchial epithelial cells. Eur Respir J,1994,7:2109-2116.
- 6. Marcet B, Libert F, Boeynaems JM, et al. Extracellular nucleotides induce COX-2 up-regulation and prostaglandin E (2) production in human A549 alveolar type II epithelial cells. Eur J Pharmacol, 2007, 566: 167-171.
- 7. Carterson AJ, Höner zu Bentrup K, Ott CM, et al .A549 lung epithelial cells grown as three-dimensional aggregates: alternative tissue culture model for Pseudomonas aeruginosa pathogenesis. Infect. Immun, 2005, 73:1129-1140.
- 8. Clemans DL, Bauer RJ, Hanson JA, et al. Induction of proinflammatory cytokines from human respiratory epithelial cells after stimulation by nontypeable Haemophilus influenzae. Infect Immun, 2000, 68:4430-4440.
- 9. Frick AG, Joseph TD, Pang LY, et al. Haemophilus influenzae stimulates ICAM-1 expression on respiratory epithelial cells. J Immunol, 2000, 164:4185-4196.